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[180713_at]

Description K09C6.4 /REP_DB=WormBase Gene ID /WP=CE11994 /TR=O16943 /GB=AAB70671.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 92      4grp_total > 49      6grp_cgc > 97      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 7.8
(-1.5)
A 		7.5
(-1.7)
A 		5
(-3)
A 		16.2
(3)
A 		11
B 4.6
(-3)
A 		25.6
(3)
A 		6.2
(-2.5)
A 		5.1
(-2.9)
A 		21
C 11.9
(-1.7)
A 		7.4
(-2.8)
A 		31.1
(3)
A 		6.5
(-3)
A 		25
avr 8.1
(-3) 		13.5
(2.4) 		14.1
(3) 		9.3
(-1.8) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 29
(2.1)
A 		8.2
(-2.6)
A 		19.9
(0.1)
A 		32.8
(3)
A 		6.4
(-3)
A 		8
(-2.6)
A 		26
B 18.2
(-1.9)
A 		40.8
(3)
A 		18.3
(-1.9)
A 		13.3
(-3)
A 		30.9
(0.8)
A 		25.3
(-0.4)
A 		28
C 2.4
(-3)
A 		10.6
(-1.4)
A 		9.2
(-1.7)
A 		12.6
(-1.1)
A 		34
(3)
A 		2.5
(-3)
A 		32
avr 16.5
(-0.7) 		19.9
(1) 		15.8
(-1) 		19.6
(0.9) 		23.8
(3) 		11.9
(-3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.9
(3)
A 		1.6
(-1.9)
A 		1.3
(-3)
A 		2.4
(1.1)
A 		2
B 1.5
(-2.5)
A 		6.3
(3)
A 		1.4
(-2.7)
A 		1.1
(-3)
A 		5
C 3.9
(-1.3)
A 		2.1
(-2.4)
A 		10.8
(3)
A 		1.1
(-3)
A 		10
avr 2.8
(-0.4) 		3.3
(0.6) 		4.5
(3) 		1.5
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 5.4
(0.3)
A 		2.9
(-2.1)
A 		4.3
(-0.8)
A 		8.3
(3)
A 		1.9
(-3)
A 		2
(-2.9)
A 		6
B 6.3
(-1.3)
A 		13.6
(3)
A 		4.4
(-2.5)
A 		3.5
(-3)
A 		12.5
(2.3)
A 		9.4
(0.5)
A 		10
C 0.9
(-3)
A 		3.2
(-1.6)
A 		2.3
(-2.2)
A 		2.5
(-2)
A 		11
(3)
A 		0.9
(-3)
A 		10
avr 4.2
(-2.4) 		6.6
(0.6) 		3.7
(-3) 		4.8
(-1.6) 		8.5
(3) 		4.1
(-2.5) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180713_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15943
Sequence Type Exemplar sequence
Representative Public ID K09C6.4 NCBI
Target Description K09C6.4 /REP_DB=WormBase Gene ID /WP=CE11994 /TR=O16943 /GB=AAB70671.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070940 NCBI Caenorhabditis elegans Serpentine Receptor, class BC (class B-like) family member (srbc-12) (srbc-12) mRNA, complete cds. 11/11 None
GENEFINDER00000012279 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:846545:852939:1 11/11 None
K09C6.4 ENSEMBL cdna:known chromosome:CEL140:V:849642:851162:1 gene:K09C6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012271 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:849950-851162(+) 98.97 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srbc-12
Ensembl K09C6.4
Entrez Gene 187201 Entrez gene
SwissProt O16943 EMBL-EBI
WormBase CE38089 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70671 Serpentine receptor, class bc (class b-like) protein 12 [Caenorhabditis elegans] ref|NP_503341.2| Serpentine Receptor, class BC (class B-like) family member (srbc-12) [Caenorhabditis elegans] 1.0E-164
blast AAB70671 Serpentine receptor, class bc (class b-like) protein 12 [Caenorhabditis elegans] ref|NP_503341.2| Serpentine Receptor, class BC (class B-like) family member (srbc-12) [Caenorhabditis elegans] 1.0E-146
blast CAE74168 Hypothetical protein CBG21841 [Caenorhabditis briggsae] 1.0E-132
blast AAB70672 Serpentine receptor, class bc (class b-like) protein 13 [Caenorhabditis elegans] ref|NP_503340.1| Serpentine Receptor, class BC (class B-like) family member (srbc-13) [Caenorhabditis elegans] 1.0E-125
blast AAB70672 Serpentine receptor, class bc (class b-like) protein 13 [Caenorhabditis elegans] ref|NP_503340.1| Serpentine Receptor, class BC (class B-like) family member (srbc-13) [Caenorhabditis elegans] 1.0E-103
blast AAC25877 Serpentine receptor, class bc (class b-like) protein 20 [Caenorhabditis elegans] ref|NP_503580.2| Serpentine Receptor, class BC (class B-like) family member (srbc-20) [Caenorhabditis elegans] 4.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 2.5E-102
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 9.1E-109
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 2.5E-102
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503341.2 5 5-27,40-62,77-99,120-142,175-194
K09C6.4 5 5-27,40-62,77-99,120-142,175-194
GENEFINDER00000012279 9 5-27,40-62,77-99,111-133,175-194,286-308,323-345,366-388,421-440
Sequence
Target Sequence 
>C. ELEGANS:180713_AT
caattgtggcactgttcatttcggtggaacgagtcgtggcggcctatttccccattcaat
accacaattatcatcaatcttttccgaaaattcctataatgataacagctattatctatg
gcttcaacgaggatatagtgctctatgtgttttgtgattttcaactaaatattcccaaaa
attgtacagcgttgggatgtgccatcaatgagtgcttttatcaatattggaccactcaca
aatcgatagtttttgcgttgatatttctgtcttctttagctttgtgctttcaactattca
tgctcaacaaacgtgaaaagaaagggaaaaatgagctaaccaagttaaaccgtcttgctc
tcatcgatggtgtcagggtgcttttagctgattttctcccgaatcttgttgccaatcagt
tgtctcggggaggattttccatttttcagagccttggcccatatgaagtggtggcaaaaa
aatttggatgtgccacggaggccattctagtgctgtacaccttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATTGTGGCACTGTTCATTTCGGT 322 179 182 Antisense
ATTTCCCCATTCAATACCACAATTA 410 17 227 Antisense
TTCTGTCTTCTTTAGCTTTGTGCTT 37 695 446 Antisense
GTGCTTTCAACTATTCATGCTCAAC 436 479 465 Antisense
TCTTGCTCTCATCGATGGTGTCAGG 547 615 534 Antisense
GGGTGCTTTTAGCTGATTTTCTCCC 376 497 557 Antisense
CTCCCGAATCTTGTTGCCAATCAGT 357 233 577 Antisense
GCCAATCAGTTGTCTCGGGGAGGAT 262 277 592 Antisense
TTTTCAGAGCCTTGGCCCATATGAA 158 675 624 Antisense
TGGATGTGCCACGGAGGCCATTCTA 499 549 666 Antisense
GCCATTCTAGTGCTGTACACCTTGC 124 281 682 Antisense
Sequence Source Affymetrix Proprietary Database