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[180712_at]

Description W03F9.3 /REP_DB=WormBase Gene ID /WP=CE14528 /TR=O16996 /GB=AAG24201.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 6.6
(-2.5)
A 		8.9
(-2.1)
A 		3.3
(-3)
A 		41.8
(3)
A 		39
B 3.3
(-3)
A 		8.5
(-1.4)
A 		6.2
(-2.1)
A 		22.3
(3)
A 		19
C 9.2
(-2.9)
A 		7.5
(-3)
A 		21.9
(-2.1)
A 		98.6
(3)
A 		91
avr 6.4
(-3) 		8.3
(-2.8) 		10.5
(-2.5) 		54.2
(3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 13.6
(-1.6)
A 		3.6
(-3)
A 		45.7
(3)
A 		6.5
(-2.6)
A 		11.1
(-1.9)
A 		14
(-1.5)
A 		42
B 2.2
(-3)
A 		3.2
(-2.8)
A 		9.1
(-1.4)
A 		8.5
(-1.5)
A 		9.4
(-1.3)
A 		27.5
(3)
A 		25
C 5
(-1.1)
A 		6.3
(0.1)
A 		9.5
(3)
A 		2.9
(-3)
A 		6.2
(0)
A 		8.4
(2)
A 		7
avr 6.9
(-2.1) 		4.4
(-3) 		21.4
(3) 		6
(-2.4) 		8.9
(-1.4) 		16.6
(1.3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.4
(-1.3)
A 		1.9
(-1.8)
A 		0.9
(-3)
A 		6.1
(3)
A 		5
B 1.1
(-3)
A 		2.1
(-1.3)
A 		1.4
(-2.5)
A 		4.7
(3)
A 		4
C 3.1
(-2.6)
A 		2.2
(-3)
A 		7.6
(-0.8)
A 		16.6
(3)
A 		14
avr 2.2
(-2.9) 		2.1
(-3) 		3.3
(-2) 		9.1
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 2.5
(-2.2)
A 		1.3
(-3)
A 		9.9
(3)
A 		1.6
(-2.8)
A 		3.3
(-1.6)
A 		3.4
(-1.5)
A 		9
B 0.8
(-3)
A 		1.1
(-2.8)
A 		2.2
(-2.1)
A 		2.3
(-2.1)
A 		3.8
(-1.1)
A 		10.3
(3)
A 		10
C 1.9
(0.5)
A 		1.9
(0.5)
A 		2.4
(1.9)
A 		0.6
(-3)
A 		2
(0.8)
A 		2.8
(3)
A 		2
avr 1.7
(-2.6) 		1.4
(-3) 		4.8
(2) 		1.5
(-2.9) 		3
(-0.7) 		5.5
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180712_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15429
Sequence Type Exemplar sequence
Representative Public ID W03F9.3 NCBI
Target Description W03F9.3 /REP_DB=WormBase Gene ID /WP=CE14528 /TR=O16996 /GB=AAG24201.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070741(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070741 NCBI Caenorhabditis elegans W03F9.3 (W03F9.3) mRNA, complete cds. 10/11 None
SNAP00000001164 ENSEMBL cdna:SNAP chromosome:CEL140:V:132379:141130:-1 11/11 None
W03F9.3 ENSEMBL cdna:known chromosome:CEL140:V:132381:132826:-1 gene:W03F9.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071128 3/11 Cross Hyb Matching Probes None
GENEFINDER00000001172 4/11 Cross Hyb Matching Probes None
SNAP00000028150 3/11 Cross Hyb Matching Probes None
GENEFINDER00000028161 3/11 Cross Hyb Matching Probes None
Y32G9A.3 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:132378-132826(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F9.3
Entrez Gene 189163 Entrez gene
SwissProt O16996 EMBL-EBI
WormBase CE35797 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY86319 Hypothetical protein W03F9.11 [Caenorhabditis elegans] 2.0E-81
blast NP_503529 Y32G9A.3 [Caenorhabditis elegans] gb|AAG53706.1| Hypothetical protein Y32G9A.3 [Caenorhabditis elegans] 1.0E-42
blast CAE62368 Hypothetical protein CBG06452 [Caenorhabditis briggsae] 2.0E-40
blast NP_503142 W03F9.3 [Caenorhabditis elegans] gb|AAG24201.2| Hypothetical protein W03F9.3 [Caenorhabditis elegans] 1.0E-39
blast NP_503529 Y32G9A.3 [Caenorhabditis elegans] gb|AAG53706.1| Hypothetical protein Y32G9A.3 [Caenorhabditis elegans] 5.0E-23
Sequence
Target Sequence 
>C. ELEGANS:180712_AT
gagaatgccggatcgctgttcggccagcgtgacctgacggcggacgtagcgacgagcatc
ctacgtcaattcgtcttgtcgacgatgcttgcaaataaagccggggtctttcaaaatgat
ccaaacttcgggaacttgcttgtgaaggaaaccaaagaggaccagctggtgttcaagatg
atggtgattccggatcccaccagcacaccacaccaagatgttgcacaagtggctcaggat
ttgaaggaactcgcggcgtcagttgcagatcgtgataagcttgaacgtacttttgctgga
tgtgtcacagccacttcaatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAATGCCGGATCGCTGTTCGGCC 508 391 16 Antisense
GCATCCTACGTCAATTCGTCTTGTC 124 309 71 Antisense
CGTCTTGTCGACGATGCTTGCAAAT 695 245 87 Antisense
AGCCGGGGTCTTTCAAAATGATCCA 518 81 114 Antisense
GATCCAAACTTCGGGAACTTGCTTG 232 415 133 Antisense
GGACCAGCTGGTGTTCAAGATGATG 121 523 174 Antisense
AGATGATGGTGATTCCGGATCCCAC 328 67 191 Antisense
CCAGCACACCACACCAAGATGTTGC 390 273 215 Antisense
ATTTGAAGGAACTCGCGGCGTCAGT 689 1 254 Antisense
GAACGTACTTTTGCTGGATGTGTCA 664 347 298 Antisense
GGATGTGTCACAGCCACTTCAATGC 152 515 313 Antisense
Sequence Source Affymetrix Proprietary Database