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[180707_at]

Description F55H12.4 /REP_DB=WormBase Gene ID /WP=CE11212 /TR=P90889 /GB=CAB03140.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 72      4grp_highest_8_8 > 11      4grp_high_8_8 > 55      6grp_highest_nonorm > 37      6grp_total > 73      4grp_total > 48      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6616
1 2 3 4 range
A 1633.7
(-3)
P 		4474.9
(-0.2)
P 		7784.9
(3)
P 		2382.2
(-2.3)
P 		6151
B 1478.5
(-3)
P 		5575.6
(0.7)
P 		8094.5
(3)
P 		2101.5
(-2.4)
P 		6616
C 1755.6
(-3)
P 		5980.6
(2.1)
P 		6725.5
(3)
P 		1927.7
(-2.8)
P 		4970
avr 1622.6
(-3) 		5343.7
(0.8) 		7535
(3) 		2137.1
(-2.5) 		5912

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5015
1 2 3 4 5 6 range
A 2610.8
(-3)
P 		5259.9
(0.4)
P 		6252.6
(1.7)
P 		5478.1
(0.7)
P 		7273.3
(3)
P 		4524.8
(-0.5)
P 		4663
B 3003.7
(-3)
P 		4708.5
(-0.7)
P 		7459.2
(3)
P 		5462.2
(0.3)
P 		6915.5
(2.3)
P 		4138.8
(-1.5)
P 		4456
C 2443.9
(-3)
P 		4468.6
(0.1)
P 		5737.4
(2)
P 		6369.8
(3)
P 		6242.3
(2.8)
P 		4373.3
(-0.1)
P 		3926
avr 2686.1
(-3) 		4812.3
(0.1) 		6483.1
(2.5) 		5770
(1.5) 		6810.4
(3) 		4345.6
(-0.6) 		4124
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2019
1 2 3 4 range
A 594.8
(-2.1)
P 		971.4
(-0.8)
P 		2035.1
(3)
P 		348.7
(-3)
P 		1686
B 471.5
(-2.9)
P 		1377.1
(0.9)
P 		1875.7
(3)
P 		447.8
(-3)
P 		1428
C 581.1
(-2.2)
P 		1719.4
(1.1)
P 		2344.1
(3)
P 		325
(-3)
P 		2019
avr 549.1
(-2.4) 		1356
(0.4) 		2085
(3) 		373.8
(-3) 		1711

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2328
1 2 3 4 5 6 range
A 481.3
(-3)
P 		1870.7
(1.9)
P 		1361.7
(0.1)
P 		1389.4
(0.2)
P 		2194.8
(3)
P 		1101.6
(-0.8)
P 		1714
B 1045.1
(-3)
P 		1567.3
(-1.2)
P 		1811.3
(-0.4)
P 		1446.7
(-1.6)
P 		2809.7
(3)
P 		1543.3
(-1.3)
P 		1765
C 905.9
(-3)
P 		1363.5
(-0.5)
P 		1460.3
(-0)
P 		1281
(-1)
P 		2019.9
(3)
P 		1464
(0)
P 		1114
avr 810.8
(-3) 		1600.5
(0.1) 		1544.4
(-0.1) 		1372.4
(-0.8) 		2341.5
(3) 		1369.6
(-0.8) 		1531
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180707_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.509
Sequence Type Exemplar sequence
Representative Public ID F55H12.4 NCBI
Target Description F55H12.4 /REP_DB=WormBase Gene ID /WP=CE11212 /TR=P90889 /GB=CAB03140.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059997(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059997 NCBI Caenorhabditis elegans F55H12.4 (F55H12.4) mRNA, complete cds. 11/11 None
SNAP00000036156 ENSEMBL cdna:SNAP chromosome:CEL140:I:8901537:8902260:-1 11/11 None
GENEFINDER00000036157 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8901537:8902260:-1 11/11 None
F55H12.4 ENSEMBL cdna:known chromosome:CEL140:I:8901441:8902261:-1 gene:F55H12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8901467-8902191(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F55H12.4
Entrez Gene 186344 Entrez gene
SwissProt P90889 EMBL-EBI
WormBase CE11212 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03140 Hypothetical protein F55H12.4 [Caenorhabditis elegans] ref|NP_492398.1| F55H12.4 [Caenorhabditis elegans] 1.0E-112
blast CAE66990 Hypothetical protein CBG12388 [Caenorhabditis briggsae] 1.0E-109
blast AAD11970 24 kDa secreted protein [Brugia malayi] 5.0E-39
Sequence
Target Sequence 
>C. ELEGANS:180707_AT
agtccttcttgtatctctagtacctcttaccgcgtcacaaatgattcgtcaatgtggctg
tggagaaattgaaggatgtatgggaagtgcgacgggtggattcatgaagtgtgctgatca
atgccagaaccacgtggcagctatgggagccaactatccagctttgagacaatgcatgtt
ggcaaaggaaccagctatcactagagctgccaactgtcaaaaatctaaccttcagaatgc
atgctcgagatccggaggtggtatggttcgcaaacgttatccagaaactcttaagcttgc
tgccttcactgaagtcaactctatcctccaaagatctggaatccaagctgaagcaaaggc
cttccttgcagtcggaaagaagtttgccacctgtgtcatgaagtgtatggatcgtggatc
taccggacactgttacaagaagcttggatgtggacttgaccttccaccagatagcattct
cgttcaatcaaccaaacaatgtgcaatcaactctggattcgatacatctggagttcgtca
actttgcaactgtgttgctggaactggtgtccgcaatctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTCCTTCTTGTATCTCTAGTACCT 378 59 27 Antisense
GAGCCAACTATCCAGCTTTGAGACA 374 385 173 Antisense
CTATCACTAGAGCTGCCAACTGTCA 387 223 221 Antisense
GATCCGGAGGTGGTATGGTTCGCAA 663 415 275 Antisense
GAAACTCTTAAGCTTGCTGCCTTCA 525 357 310 Antisense
GTCAACTCTATCCTCCAAAGATCTG 376 463 340 Antisense
TGAAGCAAAGGCCTTCCTTGCAGTC 686 573 375 Antisense
GAAAGAAGTTTGCCACCTGTGTCAT 647 359 401 Antisense
GATCGTGGATCTACCGGACACTGTT 680 417 436 Antisense
CTTCCACCAGATAGCATTCTCGTTC 595 219 487 Antisense
TGCTGGAACTGGTGTCCGCAATCTT 488 583 582 Antisense
Sequence Source Affymetrix Proprietary Database