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[180700_at]

Description F45F2.9 /REP_DB=WormBase Gene ID /WP=CE10532 /TR=Q22973 /GB=AAC48030.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 64      6grp_total > 64      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1542
1 2 3 4 range
A 789.2
(-1.7)
P 		546.9
(-2.7)
P 		486.9
(-3)
P 		1863.5
(3)
P 		1377
B 645.2
(-2.3)
P 		588.9
(-2.6)
P 		474.6
(-3)
P 		2016.4
(3)
P 		1542
C 792.3
(-2.1)
P 		606.5
(-3)
P 		637.9
(-2.8)
P 		1787.8
(3)
P 		1181
avr 742.2
(-2.1) 		580.8
(-2.8) 		533.1
(-3) 		1889.2
(3) 		1356

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 744
1 2 3 4 5 6 range
A 583.5
(-3)
P 		758.3
(-1.6)
P 		1327.6
(3)
P 		1112.9
(1.3)
P 		694.5
(-2.1)
P 		932.2
(-0.2)
P 		744
B 704.7
(-3)
P 		920.8
(0.3)
P 		1100.8
(3)
P 		849
(-0.8)
P 		806
(-1.5)
P 		1007.2
(1.6)
P 		396
C 847.7
(-1.9)
P 		870.4
(-1.6)
P 		837.6
(-2)
P 		1171.2
(1.9)
P 		753.4
(-3)
P 		1267.9
(3)
P 		515
avr 712
(-3) 		849.8
(-0.8) 		1088.7
(3) 		1044.4
(2.3) 		751.3
(-2.4) 		1069.1
(2.7) 		377
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 320
1 2 3 4 range
A 287.3
(3)
P 		118.7
(-3)
P 		127.3
(-2.7)
P 		272.8
(2.5)
P 		169
B 205.7
(-1.2)
P 		145.4
(-2.3)
P 		110
(-3)
P 		429.7
(3)
P 		320
C 262.3
(1.1)
P 		174.4
(-3)
P 		222.3
(-0.7)
P 		301.5
(3)
P 		127
avr 251.8
(0.4) 		146.2
(-3) 		153.2
(-2.8) 		334.7
(3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 317
1 2 3 4 5 6 range
A 107.6
(-3)
P 		269.7
(2.4)
P 		289.1
(3)
P 		282.3
(2.8)
P 		209.6
(0.4)
P 		227
(0.9)
P 		182
B 245.2
(-2.2)
P 		306.5
(0.2)
P 		267.3
(-1.3)
P 		224.9
(-3)
P 		327.5
(1.1)
P 		375.6
(3)
P 		151
C 314.2
(-0.1)
P 		265.6
(-1.5)
P 		213.2
(-3)
P 		235.5
(-2.4)
P 		243.8
(-2.1)
P 		424.4
(3)
P 		211
avr 222.3
(-3) 		280.6
(-0.1) 		256.5
(-1.3) 		247.6
(-1.7) 		260.3
(-1.1) 		342.3
(3) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180700_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12320
Sequence Type Exemplar sequence
Representative Public ID F45F2.9 NCBI
Target Description F45F2.9 /REP_DB=WormBase Gene ID /WP=CE10532 /TR=Q22973 /GB=AAC48030.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072790(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072790 NCBI Caenorhabditis elegans F45F2.9 (F45F2.9) mRNA, complete cds. 11/11 None
SNAP00000030765 ENSEMBL cdna:SNAP chromosome:CEL140:V:8515336:8516050:-1 11/11 None
GENEFINDER00000030778 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8515336:8516050:-1 9/11 None
F45F2.9.1 ENSEMBL cdna:known chromosome:CEL140:V:8515336:8516031:-1 gene:F45F2.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F45F2.9.2 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8515336-8516051(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45F2.9
Entrez Gene 185809 Entrez gene
SwissProt Q22973 EMBL-EBI
WormBase CE33095 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE58385 Hypothetical protein CBG01514 [Caenorhabditis briggsae] 8.0E-72
blast CAE58385 Hypothetical protein CBG01514 [Caenorhabditis briggsae] 5.0E-71
blast AAC48030 Hypothetical protein F45F2.9 [Caenorhabditis elegans] ref|NP_505191.2| F45F2.9 [Caenorhabditis elegans] 5.0E-37
blast AAC48030 Hypothetical protein F45F2.9 [Caenorhabditis elegans] ref|NP_505191.2| F45F2.9 [Caenorhabditis elegans] 7.0E-37
blast AAC48030 Hypothetical protein F45F2.9 [Caenorhabditis elegans] ref|NP_505191.2| F45F2.9 [Caenorhabditis elegans] 1.0E-36
blast CAE58385 Hypothetical protein CBG01514 [Caenorhabditis briggsae] 2.0E-30
blast BAD28004.1 putative metal-dependent phosphohydrolase HD domain-containing protein [Oryza sativa (japonica cultivar-group)] 2.0E-28
blast XP_649896 metal dependent phosphohydrolase, putative [Entamoeba histolytica HM-1:IMSS] gb|EAL44509.1| metal dependent phosphohydrolase, putative [Entamoeba histolytica HM-1:IMSS] 6.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR006674 EMBL-EBI Metal-dependent phosphohydrolase, HD subdomain 9.9E-6
Sequence
Target Sequence 
>C. ELEGANS:180700_AT
aacatctcaagcgaaccggctgggtaaaatgtggagttcctgaaccagaaactgtagcct
gccacatgtatcgaatggctgtactcgcaatggcactggaaggacaaattgatggattag
atgcaattcggactgtgaagatggctcttgctatcaatacaatcgcttcattcgttccaa
atgttggcgaggagtggacaatgctctggaaagaatacgaagaagcttcatcattgacag
ctagagttgtgaaacatctcgacaagtttgacatgattgtgcaagctgataaatatgaga
aaactcacgaaattgacctacaacagttcttcacgagcacagtaggcgttttgaaaatgg
aaccgtttgccacgtgggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACATCTCAAGCGAACCGGCTGGGT 615 125 62 Antisense
GAAACTGTAGCCTGCCACATGTATC 257 357 109 Antisense
CATGTATCGAATGGCTGTACTCGCA 657 209 126 Antisense
GTACTCGCAATGGCACTGGAAGGAC 319 457 142 Antisense
GGATTAGATGCAATTCGGACTGTGA 568 509 175 Antisense
GACTGTGAAGATGGCTCTTGCTATC 65 377 192 Antisense
CAATACAATCGCTTCATTCGTTCCA 168 181 216 Antisense
GGCGAGGAGTGGACAATGCTCTGGA 448 541 247 Antisense
CGAAATTGACCTACAACAGTTCTTC 73 253 369 Antisense
ACAGTTCTTCACGAGCACAGTAGGC 528 107 384 Antisense
AATGGAACCGTTTGCCACGTGGGAT 287 163 417 Antisense
Sequence Source Affymetrix Proprietary Database