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[180690_at]

Description F35E12.4 /REP_DB=WormBase Gene ID /WP=CE09919 /TR=O02359 /GB=CAB04274.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 5.7
(-3)
A 		7.1
(-2.9)
A 		10.2
(-2.6)
A 		75
(3)
A 		69
B 31.7
(3)
A 		3.7
(-3)
A 		30.3
(2.7)
A 		5.6
(-2.6)
A 		28
C 27.3
(3)
A 		5.1
(-3)
A 		8.6
(-2.1)
A 		14.6
(-0.4)
A 		22
avr 21.6
(0.7) 		5.3
(-3) 		16.4
(-0.5) 		31.7
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 10.2
(-2.9)
A 		9.6
(-3)
A 		37
(2.4)
A 		19.6
(-1)
A 		10.6
(-2.8)
A 		40.2
(3)
A 		31
B 32.2
(-0.4)
A 		31.9
(-0.4)
A 		12.3
(-3)
A 		31.8
(-0.4)
A 		41.3
(0.8)
A 		57.7
(3)
M 		45
C 4
(-2.8)
A 		71.8
(3)
A 		15.7
(-1.8)
A 		53.7
(1.4)
A 		1.9
(-3)
A 		46.6
(0.8)
P 		70
avr 15.5
(-3) 		37.8
(1.1) 		21.7
(-1.9) 		35
(0.6) 		17.9
(-2.6) 		48.2
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.1
(-2.6)
A 		1.5
(-3)
A 		2.7
(-2.2)
A 		11
(3)
A 		10
B 10.1
(3)
A 		0.9
(-3)
A 		7
(1)
A 		1.2
(-2.8)
A 		9
C 9
(3)
A 		1.5
(-3)
A 		3
(-1.8)
A 		2.5
(-2.2)
A 		8
avr 7.1
(3) 		1.3
(-3) 		4.2
(0) 		4.9
(0.7) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 1.9
(-3)
A 		3.4
(-1.9)
A 		8.1
(1.7)
A 		5
(-0.6)
A 		3.2
(-2)
A 		9.8
(3)
A 		8
B 11.2
(-0.3)
A 		10.6
(-0.5)
A 		3
(-3)
A 		8.4
(-1.2)
A 		16.8
(1.5)
A 		21.5
(3)
M 		19
C 1.5
(-2.7)
A 		21.9
(3)
A 		4
(-2)
A 		10.8
(-0.1)
A 		0.6
(-3)
A 		15.6
(1.2)
P 		21
avr 4.9
(-3) 		12
(1) 		5
(-2.9) 		8.1
(-1.2) 		6.9
(-1.9) 		15.6
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180690_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12954
Sequence Type Exemplar sequence
Representative Public ID F35E12.4 NCBI
Target Description F35E12.4 /REP_DB=WormBase Gene ID /WP=CE09919 /TR=O02359 /GB=CAB04274.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073999
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000032243 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13729622:13738741:-1 None
F35E12.4 ENSEMBL cdna:known chromosome:CEL140:V:13734096:13735977:-1 gene:F35E12.4 None
SNAP00000032231 ENSEMBL cdna:SNAP chromosome:CEL140:V:13735066:13735977:-1 None
NM_073999 NCBI Caenorhabditis elegans F35E12.4 (F35E12.4) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032230 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13734095-13735977(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E12.4
Entrez Gene 185306 Entrez gene
SwissProt O02359 EMBL-EBI
WormBase CE35522 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04274 Hypothetical protein F35E12.4 [Caenorhabditis elegans] ref|NP_506400.2| F35E12.4 [Caenorhabditis elegans] 0.0
blast CAB04275 Hypothetical protein F35E12.7a [Caenorhabditis elegans] ref|NP_001023877.1| F35E12.7a [Caenorhabditis elegans] 0.0
blast CAI91171 Hypothetical protein F35E12.7c [Caenorhabditis elegans] ref|NP_001023879.1| F35E12.7c [Caenorhabditis elegans] 0.0
blast CAI91172 Hypothetical protein F35E12.7d [Caenorhabditis elegans] ref|NP_001023880.1| F35E12.7d [Caenorhabditis elegans] 0.0
blast CAB04275 Hypothetical protein F35E12.7a [Caenorhabditis elegans] ref|NP_001023877.1| F35E12.7a [Caenorhabditis elegans] 2.0E-96
blast CAI91170 Hypothetical protein F35E12.7b [Caenorhabditis elegans] ref|NP_001023878.1| F35E12.7b [Caenorhabditis elegans] 2.0E-96
blast CAB04274 Hypothetical protein F35E12.4 [Caenorhabditis elegans] ref|NP_506400.2| F35E12.4 [Caenorhabditis elegans] 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 6.9E-11
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 3.6E-26
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 6.9E-11
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 2.6E-26
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.7E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506400.2 1 365-387
F35E12.4 1 365-387
GENEFINDER00000032243 1 1407-1429
Sequence
Target Sequence 
>C. ELEGANS:180690_AT
aaatccaggaaatgaccccgacgcagaagcttcaaccaatgatcaagtatccatattctc
ctaacacctacaaaaatattcatcgattaattccatcccagattgcgacttttcatgttc
aaagcgcatgtattcacttttccattaaaccgaattatcagcaagtagtcggtactacta
gtgcctcccaatctgtaagttcgattgttccgtctgaacgggtttacacaactcatcctg
acattacctctaactcctcaacaacactatcaactacattaaaaattaaaggaagtaaac
agccatgtaagaatgttttattttcggagaaagttcttcaattgaagttatacttaaagt
atctttcgaccatgcactttcaatatactctttttcttttttggttttttacaactgata
ctgatatcgaaaaaaaattgccaattcatgttttttctaaaattcaaaattttcaggcta
gctcttcgatgacaaccggttacgctgaagcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCCAGGAAATGACCCCGACGCA 669 115 674 Antisense
GATCAAGTATCCATATTCTCCTAAC 123 423 714 Antisense
ATTCATCGATTAATTCCATCCCAGA 32 13 751 Antisense
ATCCCAGATTGCGACTTTTCATGTT 133 39 768 Antisense
TCTGTAAGTTCGATTGTTCCGTCTG 118 607 865 Antisense
TACACAACTCATCCTGACATTACCT 395 641 898 Antisense
TCTAACTCCTCAACAACACTATCAA 452 613 922 Antisense
TATCTTTCGACCATGCACTTTCAAT 675 659 1033 Antisense
GCACTTTCAATATACTCTTTTTCTT 612 315 1047 Antisense
CAGGCTAGCTCTTCGATGACAACCG 271 209 1147 Antisense
GACAACCGGTTACGCTGAAGCAACA 632 363 1164 Antisense
Sequence Source Affymetrix Proprietary Database