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[180687_at]

Description K10B3.5 /REP_DB=WormBase Gene ID /WP=CE07368 /TR=Q21406 /GB=AAB53872.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 72      6grp_total > 81      4grp_total > 38      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 384
(3)
P 		285.6
(-3)
P 		308.9
(-1.6)
P 		366.8
(2)
P 		98
B 429.2
(3)
P 		354.6
(-1.2)
P 		321.6
(-3)
P 		344.4
(-1.7)
P 		108
C 347.8
(3)
P 		342.8
(2.6)
P 		278.4
(-3)
P 		313.2
(0)
P 		69
avr 387
(3) 		327.7
(-1.2) 		303
(-3) 		341.5
(-0.3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 331.3
(-3)
P 		400.2
(-0.4)
P 		355.1
(-2.1)
P 		463.1
(1.9)
P 		491.3
(3)
P 		386.5
(-0.9)
P 		160
B 351.9
(-2.7)
P 		393.1
(-1.6)
P 		411.5
(-1.1)
P 		558.4
(3)
P 		407.4
(-1.2)
P 		340.8
(-3)
P 		218
C 320.3
(-3)
P 		362.1
(-1.7)
P 		413.8
(-0.2)
P 		520.8
(3)
P 		354.8
(-2)
P 		332.6
(-2.6)
P 		201
avr 334.5
(-3) 		385.1
(-1.3) 		393.5
(-1) 		514.1
(3) 		417.8
(-0.2) 		353.3
(-2.4) 		180
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 139.8
(3)
P 		62
(-2.4)
P 		80.8
(-1.1)
P 		53.7
(-3)
P 		86
B 136.9
(3)
P 		87.6
(-1.7)
P 		74.5
(-2.9)
P 		73.4
(-3)
P 		64
C 115.1
(3)
P 		98.6
(1.4)
P 		97
(1.3)
P 		52.8
(-3)
P 		62
avr 130.6
(3) 		82.7
(-1.1) 		84.1
(-1) 		60
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 61.1
(-3)
P 		142.3
(2.6)
P 		77.3
(-1.9)
P 		117.5
(0.9)
P 		148.2
(3)
P 		94.1
(-0.7)
P 		87
B 122.5
(-0.9)
P 		130.8
(-0.2)
P 		99.9
(-3)
P 		147.9
(1.4)
P 		165.5
(3)
P 		127.1
(-0.5)
P 		66
C 118.7
(3)
P 		110.5
(-0.5)
P 		105.3
(-2.7)
P 		104.7
(-3)
P 		114.8
(1.3)
P 		111.3
(-0.2)
P 		14
avr 100.8
(-2.2) 		127.9
(1.2) 		94.2
(-3) 		123.4
(0.6) 		142.8
(3) 		110.8
(-1) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180687_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18238
Sequence Type Exemplar sequence
Representative Public ID K10B3.5 NCBI
Target Description K10B3.5 /REP_DB=WormBase Gene ID /WP=CE07368 /TR=Q21406 /GB=AAB53872.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076131(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076131 NCBI Caenorhabditis elegans K10B3.5 (K10B3.5) mRNA, complete cds. 11/11 None
SNAP00000032570 ENSEMBL cdna:SNAP chromosome:CEL140:X:3103588:3110606:-1 11/11 None
GENEFINDER00000032578 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3103588:3106243:-1 11/11 None
K10B3.5 ENSEMBL cdna:known chromosome:CEL140:X:3103241:3106270:-1 gene:K10B3.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3103587-3106270(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K10B3.5
Entrez Gene 180599 Entrez gene
SwissProt Q21406 EMBL-EBI
WormBase CE07368 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53872 Hypothetical protein K10B3.5 [Caenorhabditis elegans] ref|NP_508532.1| K10B3.5 [Caenorhabditis elegans] 0.0
blast AAB53873 Hypothetical protein K10B3.6a [Caenorhabditis elegans] ref|NP_001024784.1| K10B3.6a [Caenorhabditis elegans] 0.0
blast CAE68379 Hypothetical protein CBG14135 [Caenorhabditis briggsae] 0.0
blast CAE68378 Hypothetical protein CBG14134 [Caenorhabditis briggsae] 7.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR004129 EMBL-EBI Glycerophosphoryl diester phosphodiesterase 9.6E-91
Sequence
Target Sequence 
>C. ELEGANS:180687_AT
ggagctttgtgagcagccatcttgctcaatctggcaagttgaccggtacaacgaaggaca
atgagactatcaaaaaggaggccaaagtgctcttcaacccattagcaaaccgagcagtgg
gttcgcgtcaaagtggagttactaatgcttctgctagcaaagtttgtgtcgacgaatcag
cttccatcaacgacctgctcaagcaagcttatatttatggcccgtcagttcaccgcctac
ccatcaactccgttccaatgtacaaagtcattcgttgctatgagcccgattttttctcgg
aggaaatgcaaaatcgatatttagcaagaaacccaccggttgcacctgatgcggcagttt
ttcgaccaaaaatgccacacatcgttttgcaatcgcccagtaaaaaacagtttactccag
ctactttcaacaatcgagttagctacatcttaaagccgggaacgaaagctggaacaaccg
ttagactcattagttccacaaaaacccaaccacgacctgtaaatagtggcatcgcttatc
aatcttctgacgttcagcctcaacttatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCTTTGTGAGCAGCCATCTTGC 284 521 1238 Antisense
CCAAAGTGCTCTTCAACCCATTAGC 689 275 1319 Antisense
TGTGTCGACGAATCAGCTTCCATCA 386 555 1402 Antisense
TATATTTATGGCCCGTCAGTTCACC 11 661 1447 Antisense
CCATCAACTCCGTTCCAATGTACAA 579 269 1478 Antisense
GTTGCTATGAGCCCGATTTTTTCTC 383 439 1511 Antisense
GTTGCACCTGATGCGGCAGTTTTTC 558 439 1576 Antisense
TGCCACACATCGTTTTGCAATCGCC 445 585 1610 Antisense
AACAGTTTACTCCAGCTACTTTCAA 372 137 1643 Antisense
GCATCGCTTATCAATCTTCTGACGT 62 311 1766 Antisense
TCTGACGTTCAGCCTCAACTTATGA 341 609 1783 Antisense
Sequence Source Affymetrix Proprietary Database