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[180680_at]

Description K04A8.9 /REP_DB=WormBase Gene ID /WP=CE11716 /TR=Q94256 /GB=AAC48055.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 10      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 19
(-2.7)
A 		63.1
(-1.4)
A 		10.4
(-3)
A 		205.1
(3)
P 		195
B 41.7
(-2.9)
A 		37.8
(-3)
A 		72
(-2.2)
A 		305.5
(3)
P 		268
C 46.1
(-2.6)
A 		60.7
(-2.2)
A 		32.4
(-3)
P 		254.1
(3)
P 		222
avr 35.6
(-3) 		53.9
(-2.5) 		38.3
(-2.9) 		254.9
(3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 52.4
(-3)
A 		57.6
(-2.8)
A 		80.2
(-2.1)
P 		140.3
(-0.3)
P 		248.5
(3)
P 		166.2
(0.5)
P 		196
B 49.1
(-2.9)
A 		44.9
(-3)
A 		47.1
(-2.9)
A 		128.9
(-0.3)
P 		229.4
(3)
P 		185.3
(1.6)
P 		185
C 45.8
(-2)
P 		51.4
(-1.9)
A 		12
(-3)
A 		180.3
(1.7)
P 		224.7
(3)
P 		190
(2)
P 		213
avr 49.1
(-2.9) 		51.3
(-2.8) 		46.4
(-3) 		149.8
(0.3) 		234.2
(3) 		180.5
(1.3) 		188
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 6.9
(-2.1)
A 		13.7
(-0.6)
A 		2.7
(-3)
A 		30
(3)
P 		27
B 13.3
(-2.6)
A 		9.3
(-3)
A 		16.7
(-2.2)
A 		65.1
(3)
P 		56
C 15.2
(-2.3)
A 		17.5
(-1.8)
A 		11.3
(-3)
P 		42.8
(3)
P 		32
avr 11.8
(-2.7) 		13.5
(-2.4) 		10.2
(-3) 		46
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 9.7
(-3)
A 		20.5
(-2)
A 		17.5
(-2.3)
P 		35.6
(-0.6)
P 		75
(3)
P 		40.5
(-0.2)
P 		65
B 17.1
(-2.6)
A 		14.9
(-2.7)
A 		11.4
(-3)
A 		34.1
(-1.3)
P 		93.2
(3)
P 		69.1
(1.2)
P 		82
C 17
(-1.8)
P 		15.7
(-1.9)
A 		3.1
(-3)
A 		36.3
(-0.1)
P 		72.7
(3)
P 		63.6
(2.2)
P 		70
avr 14.6
(-2.7) 		17
(-2.5) 		10.7
(-3) 		35.3
(-0.9) 		80.3
(3) 		57.7
(1.1) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180680_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11544
Sequence Type Exemplar sequence
Representative Public ID K04A8.9 NCBI
Target Description K04A8.9 /REP_DB=WormBase Gene ID /WP=CE11716 /TR=Q94256 /GB=AAC48055.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072265(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072265 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-19) (spp-19) mRNA, complete cds. 11/11 None
K04A8.9 ENSEMBL cdna:known chromosome:CEL140:V:6561360:6563158:-1 gene:K04A8.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001685 4/11 Cross Hyb Matching Probes None
GENEFINDER00000001693 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6561359-6563158(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-19
Ensembl K04A8.9
Entrez Gene 186974 Entrez gene
SwissProt Q94256 EMBL-EBI
WormBase CE11716 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48055 Saposin-like protein family protein 19 [Caenorhabditis elegans] ref|NP_504666.1| SaPosin-like Protein family member (spp-19) [Caenorhabditis elegans] 3.0E-89
blast CAE71908 Hypothetical protein CBG18970 [Caenorhabditis briggsae] 7.0E-21
Sequence
Target Sequence 
>C. ELEGANS:180680_AT
aggcttttctcaatgtaaccctctgcaccagtcttgatttttcaaatattacaattgacg
acgctcttcctggagaaattaaatgcacatgctctagggacttctgcaatggaagcttcg
aaaacgatcctaacacatcttctagaatttttagcacaatatttggattgattattacaa
ttatcaaccccccgaatatagtttgtcagttgtgcacaagtggcctctacctggttcagt
ctcaattgcttgtgctggaaaatgaagttaaaaacaatctaaagccatttattactaacg
tttgtcaatcagctccaacaggaataccaatagtgaaagctttatgtgatgtgctcaaag
atgatctcttggatgccattttaactttgatcaatggaattagagagcaagctgatccga
ataccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGCTTTTCTCAATGTAACCCTCTG 395 53 47 Antisense
CTCTGCACCAGTCTTGATTTTTCAA 561 231 67 Antisense
TGACGACGCTCTTCCTGGAGAAATT 663 569 102 Antisense
AATGCACATGCTCTAGGGACTTCTG 368 165 128 Antisense
AAACGATCCTAACACATCTTCTAGA 663 125 168 Antisense
GATTATTACAATTATCAACCCCCCG 369 429 216 Antisense
CAACCCCCCGAATATAGTTTGTCAG 415 143 231 Antisense
GTCAGTTGTGCACAAGTGGCCTCTA 21 467 251 Antisense
ACCTGGTTCAGTCTCAATTGCTTGT 186 89 275 Antisense
AACGTTTGTCAATCAGCTCCAACAG 551 143 343 Antisense
GAGAGCAAGCTGATCCGAATACCGT 476 393 449 Antisense
Sequence Source Affymetrix Proprietary Database