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[180669_s_at]

Description Y4C6B.6 /REP_DB=WormBase Gene ID /WP=CE28522 /TR=Q9UB00 /GB=AAD14768.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 31      4grp_high_8_8 > 60      6grp_highest_nonorm > 42      6grp_total > 42      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 933
1 2 3 4 range
A 536.6
(-0)
P 		533.2
(-0.1)
P 		963.1
(3)
P 		123.2
(-3)
P 		840
B 562.3
(0.2)
P 		600.4
(0.5)
P 		985.9
(3)
P 		72.1
(-3)
A 		914
C 689.7
(1)
P 		700.3
(1.1)
P 		996.2
(3)
P 		63.2
(-3)
A 		933
avr 596.2
(0.4) 		611.3
(0.5) 		981.7
(3) 		86.2
(-3) 		896

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1099
1 2 3 4 5 6 range
A 870.6
(0.8)
P 		918.1
(1.1)
P 		1253.6
(3)
P 		730.2
(0)
P 		395.7
(-1.9)
P 		201.7
(-3)
P 		1052
B 857
(0.9)
P 		853.2
(0.9)
P 		1178.4
(3)
P 		679.2
(-0.2)
P 		419.8
(-1.8)
P 		239.7
(-3)
P 		939
C 824.2
(0.4)
P 		874.7
(0.7)
P 		1301
(3)
P 		683.9
(-0.4)
P 		420.8
(-1.8)
P 		204.7
(-3)
P 		1096
avr 850.6
(0.7) 		882
(0.9) 		1244.3
(3) 		697.8
(-0.2) 		412.1
(-1.9) 		215.4
(-3) 		1029
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 337
1 2 3 4 range
A 195.4
(1.6)
P 		115.7
(-0.5)
P 		251.8
(3)
P 		18
(-3)
P 		234
B 179.3
(1.6)
P 		148.3
(0.7)
P 		228.5
(3)
P 		15.4
(-3)
A 		213
C 228.3
(0.9)
P 		201.3
(0.4)
P 		347.2
(3)
P 		10.7
(-3)
A 		337
avr 201
(1.3) 		155.1
(0.2) 		275.8
(3) 		14.7
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 160.5
(-0.6)
P 		326.5
(3)
P 		273
(1.8)
P 		185.2
(-0.1)
P 		119.4
(-1.5)
P 		49.1
(-3)
P 		277
B 298.2
(3)
P 		284
(2.6)
P 		286.1
(2.7)
P 		179.9
(-0.4)
P 		170.6
(-0.7)
P 		89.4
(-3)
P 		209
C 305.5
(2.4)
P 		266.9
(1.5)
P 		331.1
(3)
P 		137.5
(-1.4)
P 		136.2
(-1.5)
P 		68.5
(-3)
P 		263
avr 254.7
(1.9) 		292.5
(2.9) 		296.7
(3) 		167.5
(-0.4) 		142.1
(-1.1) 		69
(-3) 		228
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180669_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11109
Sequence Type Exemplar sequence
Representative Public ID Y4C6B.6 NCBI
Target Description Y4C6B.6 /REP_DB=WormBase Gene ID /WP=CE28522 /TR=Q9UB00 /GB=AAD14768.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068384(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068384 NCBI Caenorhabditis elegans Y4C6B.6 (Y4C6B.6) mRNA, complete cds. 11/11 None
SNAP00000011099 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5344622:5347149:-1 11/11 None
GENEFINDER00000011106 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5344622:5349406:-1 11/11 None
Y4C6B.6 ENSEMBL cdna:known chromosome:CEL140:IV:5344588:5350918:-1 gene:Y4C6B.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5344627-5348424(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y4C6B.6
Entrez Gene 177314 Entrez gene
SwissProt Q9UB00 EMBL-EBI
WormBase CE28522 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6665 sphingolipid metabolism inferred from electronic annotation QuickGO AmiGO
7040 lysosome organization and biogenesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5764 lysosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4348 glucosylceramidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD14768 Hypothetical protein Y4C6B.6 [Caenorhabditis elegans] ref|NP_500785.1| Y4C6B.6 [Caenorhabditis elegans] 0.0
blast CAE72726 Hypothetical protein CBG19964 [Caenorhabditis briggsae] 0.0
blast CAB02924 Hypothetical protein F11E6.1a [Caenorhabditis elegans] emb|CAA20283.1| Hypothetical protein F11E6.1a [Caenorhabditis elegans] ref|NP_503119.1| F11E6.1a [Caenorhabditis elegans] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:180669_S_AT
ttttaacggatccaactgctgctcaatacgtccacggaattgcaattcattggtatgaag
acttcattgacccagcaaccgtactcactgaaactcacgagaagttcccagactatttcc
ttttggcaactgaagcatgtgctggatacttcccagcagatggaccaaaattgggatcat
ggtctagagctgaacaatatgcaaatgatttgattaaagatatgggaaattgggttggag
gatgggttgattggaattatattcttgatttacaaggaggaccaaatcttgccaaaaact
ttgtcgattcaacaataatcgtgaatgcaactgctcaagagtactacaaacaaccaattt
ggcatgtaatggctcaattcagtaaatttgtgaaaccgggagctattcgtgttggaataa
acattattgagaaatcagttgatgttgagggtctttcatttttgaatcaagatggtacca
aaactgtggttttattgaataaaaatgaggtactcagcttcgacgtggcaatcagtgacg
tcagtgctccaaatgtgatctatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTAACGGATCCAACTGCTGCTCA 417 675 929 Antisense
TGCTGCTCAATACGTCCACGGAATT 50 583 945 Antisense
TGAAGACTTCATTGACCCAGCAACC 408 575 984 Antisense
AGCAACCGTACTCACTGAAACTCAC 166 75 1002 Antisense
GAAACTCACGAGAAGTTCCCAGACT 53 357 1018 Antisense
GTTCCCAGACTATTTCCTTTTGGCA 205 441 1032 Antisense
GAAGCATGTGCTGGATACTTCCCAG 200 341 1060 Antisense
GAAACCGGGAGCTATTCGTGTTGGA 281 359 1320 Antisense
GATGTTGAGGGTCTTTCATTTTTGA 221 407 1369 Antisense
TACTCAGCTTCGACGTGGCAATCAG 148 647 1439 Antisense
GTCAGTGCTCCAAATGTGATCTATG 207 461 1468 Antisense
Sequence Source Affymetrix Proprietary Database