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[180667_at]

Description T09B4.4 /REP_DB=WormBase Gene ID /WP=CE13463 /TR=O02159 /GB=AAB53013.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 12      4grp_high_8_8 > 31      6grp_highest_nonorm > 53      6grp_total > 62      4grp_total > 32      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 626
1 2 3 4 range
A 198.3
(-3)
P 		475.8
(1.3)
P 		577.9
(2.9)
P 		585
(3)
P 		387
B 318.9
(-3)
P 		574
(0.6)
P 		742.5
(3)
P 		625.6
(1.3)
P 		424
C 287.5
(-3)
P 		515.1
(-0.5)
P 		579.7
(0.3)
P 		824.4
(3)
P 		537
avr 268.2
(-3) 		521.6
(0.7) 		633.4
(2.3) 		678.3
(3) 		410

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 950
1 2 3 4 5 6 range
A 409
(-3)
P 		721
(-1)
P 		1359
(3)
P 		1127.5
(1.5)
P 		508.6
(-2.4)
P 		413.2
(-3)
P 		950
B 441.5
(-3)
P 		868.3
(-0.2)
P 		1356.4
(3)
P 		1011.8
(0.7)
P 		778.8
(-0.8)
P 		471.1
(-2.8)
P 		915
C 440.1
(-3)
P 		769
(-0.7)
P 		834.5
(-0.2)
P 		1296.3
(3)
P 		737.1
(-0.9)
P 		480.4
(-2.7)
P 		856
avr 430.2
(-3) 		786.1
(-0.2) 		1183.3
(3) 		1145.2
(2.7) 		674.8
(-1.1) 		454.9
(-2.8) 		753
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 72.2
(-3)
P 		103.3
(-0.6)
P 		151.1
(3)
P 		85.6
(-2)
P 		79
B 101.7
(-3)
P 		141.8
(0.4)
P 		172.1
(3)
P 		133.3
(-0.3)
P 		70
C 95.2
(-3)
P 		148.1
(-0)
P 		202
(3)
P 		139
(-0.5)
P 		107
avr 89.7
(-3) 		131.1
(-0.1) 		175.1
(3) 		119.3
(-0.9) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 75.4
(-3)
P 		256.4
(1.9)
P 		296
(3)
P 		286
(2.7)
P 		153.5
(-0.9)
P 		100.6
(-2.3)
P 		221
B 153.6
(-3)
P 		289
(1.6)
P 		329.4
(3)
P 		268
(0.9)
P 		316.4
(2.6)
P 		175.7
(-2.2)
P 		176
C 163.1
(-2.9)
P 		234.7
(1.4)
P 		212.4
(0.1)
P 		260.7
(3)
P 		238.5
(1.7)
P 		160.8
(-3)
P 		100
avr 130.7
(-3) 		260
(2.2) 		279.3
(3) 		271.6
(2.7) 		236.1
(1.3) 		145.7
(-2.4) 		149
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180667_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2424
Sequence Type Exemplar sequence
Representative Public ID T09B4.4 NCBI
Target Description T09B4.4 /REP_DB=WormBase Gene ID /WP=CE13463 /TR=O02159 /GB=AAB53013.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059377(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059377 NCBI Caenorhabditis elegans T09B4.4 (T09B4.4) mRNA, complete cds. 11/11 None
SNAP00000036038 ENSEMBL cdna:SNAP chromosome:CEL140:I:6176152:6176737:1 11/11 None
GENEFINDER00000036048 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6176152:6182017:1 11/11 None
T09B4.4 ENSEMBL cdna:known chromosome:CEL140:I:6176083:6176771:1 gene:T09B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6176080-6176666(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T09B4.4
Entrez Gene 188316 Entrez gene
SwissProt O02159 EMBL-EBI
WormBase CE36196 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53013 Hypothetical protein T09B4.4 [Caenorhabditis elegans] ref|NP_491778.2| T09B4.4 [Caenorhabditis elegans] 7.0E-76
blast AAB53013 Hypothetical protein T09B4.4 [Caenorhabditis elegans] ref|NP_491778.2| T09B4.4 [Caenorhabditis elegans] 4.0E-73
blast CAE67400 Hypothetical protein CBG12886 [Caenorhabditis briggsae] 3.0E-67
blast CAE67400 Hypothetical protein CBG12886 [Caenorhabditis briggsae] 7.0E-65
blast XP_625045 PREDICTED: similar to ENSANGP00000021588 [Apis mellifera] 9.0E-17
blast XP_625045 PREDICTED: similar to ENSANGP00000021588 [Apis mellifera] 1.0E-16
Sequence
Target Sequence 
>C. ELEGANS:180667_AT
atgactgaatttttctcgcagaagcagattgatgtactaagaacagattctcaacttcga
tgtgctctccgaagtcttggatattctccaactgcttccaaaactgatatttatttcaag
aagcttaataaaaaaccaatcgaatttgcaacatttttggacatttgcaaagatgaacaa
aatagtccgaatccactgacggagattatcaaagcgttaagtggattggatagaaataaa
actcgcgcaatgccatctcgtgagcttgccgcaatactttcacacgttggggagcaaatg
agtccagaggagattaaatatcttttatcaaaagttgaggtcaacggaatggttcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTGAATTTTTCTCGCAGAAGC 492 45 13 Antisense
TCGCAGAAGCAGATTGATGTACTAA 290 601 28 Antisense
AGATTCTCAACTTCGATGTGCTCTC 574 65 57 Antisense
TGTGCTCTCCGAAGTCTTGGATATT 362 557 73 Antisense
CTTGGATATTCTCCAACTGCTTCCA 177 217 88 Antisense
GTCCGAATCCACTGACGGAGATTAT 6 473 197 Antisense
TAAAACTCGCGCAATGCCATCTCGT 127 641 249 Antisense
ATCTCGTGAGCTTGCCGCAATACTT 222 33 267 Antisense
GCCGCAATACTTTCACACGTTGGGG 231 283 280 Antisense
GTTGGGGAGCAAATGAGTCCAGAGG 206 437 298 Antisense
AGTTGAGGTCAACGGAATGGTTCCT 348 65 345 Antisense
Sequence Source Affymetrix Proprietary Database