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[180663_s_at]

Description T20D4.4 /REP_DB=WormBase Gene ID /WP=CE13786 /TR=P91471 /GB=AAB37593.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 62      4grp_total > 08      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 2.9
(-0.4)
A 		4.7
(3)
A 		1.5
(-3)
A 		1.8
(-2.4)
A 		3
B 11.3
(-1)
A 		7.3
(-3)
A 		19.3
(3)
A 		13.8
(0.3)
A 		12
C 3.3
(-2.9)
A 		2.6
(-3)
A 		12.2
(-1.5)
A 		41.2
(3)
A 		39
avr 5.8
(-2.6) 		4.9
(-3) 		11
(-0.4) 		18.9
(3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 0.6
(-3)
A 		0.9
(-2.9)
A 		24
(3)
A 		14.3
(0.5)
A 		12.1
(-0.1)
A 		1.7
(-2.7)
A 		23
B 1
(-3)
A 		2.9
(-2.5)
A 		26.2
(3)
A 		1.5
(-2.9)
A 		5.6
(-1.9)
A 		4.9
(-2.1)
A 		25
C 0.9
(-3)
A 		7
(-2)
A 		3.4
(-2.6)
A 		36.3
(3)
A 		5.8
(-2.2)
A 		2
(-2.8)
A 		35
avr 0.8
(-3) 		3.6
(-2) 		17.9
(3) 		17.4
(2.8) 		7.8
(-0.5) 		2.9
(-2.3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.1
(3)
A 		1
(2.3)
A 		0.4
(-2.3)
A 		0.3
(-3)
A 		1
B 3.6
(1)
A 		1.8
(-3)
A 		4.5
(3)
A 		2.9
(-0.6)
A 		3
C 1.1
(-2.6)
A 		0.7
(-3)
A 		4.3
(0.5)
A 		6.9
(3)
A 		6
avr 1.9
(-1.1) 		1.2
(-3) 		3.1
(2.2) 		3.4
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.1
(-3)
A 		0.3
(-2.8)
A 		5.2
(3)
A 		3.6
(1.1)
A 		3.6
(1.1)
A 		0.4
(-2.6)
A 		5
B 0.4
(-3)
A 		1
(-2.4)
A 		6.4
(3)
A 		0.4
(-3)
A 		2.3
(-1.1)
A 		1.8
(-1.6)
A 		6
C 0.3
(-3)
A 		2.1
(-1.5)
A 		0.9
(-2.5)
A 		7.3
(3)
A 		1.9
(-1.6)
A 		0.7
(-2.7)
A 		7
avr 0.3
(-3) 		1.1
(-1.8) 		4.2
(3) 		3.8
(2.4) 		2.6
(0.5) 		1
(-1.9) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180663_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15839
Sequence Type Exemplar sequence
Representative Public ID T20D4.4 NCBI
Target Description T20D4.4 /REP_DB=WormBase Gene ID /WP=CE13786 /TR=P91471 /GB=AAB37593.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071557(10), NM_071556(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071557 NCBI Caenorhabditis elegans T20D4.3 (T20D4.3) mRNA, complete cds. 10/11 None
NM_071556 NCBI Caenorhabditis elegans T20D4.4 (T20D4.4) mRNA, complete cds. 10/11 None
GENEFINDER00000008859 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3413170:3422452:1 11/11 None
T20D4.4 ENSEMBL cdna:known chromosome:CEL140:V:3415819:3417578:1 gene:T20D4.4 10/11 None
T20D4.3 ENSEMBL cdna:known chromosome:CEL140:V:3418089:3422559:1 gene:T20D4.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000008852 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3415818-3417580(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl T20D4.4
T20D4.3
Entrez Gene 188639 Entrez gene
188640 Entrez gene
SwissProt P91472 EMBL-EBI
P91471 EMBL-EBI
WormBase CE35776 Wormbase
CE35777 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37594 Hypothetical protein T20D4.3 [Caenorhabditis elegans] ref|NP_503958.2| T20D4.3 [Caenorhabditis elegans] 0.0
blast AAB37592 Hypothetical protein T20D4.5 [Caenorhabditis elegans] ref|NP_503956.2| T20D4.5 [Caenorhabditis elegans] 0.0
blast AAB37592 Hypothetical protein T20D4.5 [Caenorhabditis elegans] ref|NP_503956.2| T20D4.5 [Caenorhabditis elegans] 1.0E-171
blast AAB37593 Hypothetical protein T20D4.4 [Caenorhabditis elegans] ref|NP_503957.2| T20D4.4 [Caenorhabditis elegans] 1.0E-119
blast AAB37593 Hypothetical protein T20D4.4 [Caenorhabditis elegans] ref|NP_503957.2| T20D4.4 [Caenorhabditis elegans] 1.0E-111
blast AAB37594 Hypothetical protein T20D4.3 [Caenorhabditis elegans] ref|NP_503958.2| T20D4.3 [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 3.6E-44
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.5E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.3E-43
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 4.7E-44
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 7.9E-46
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.8E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 3.6E-44
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.5E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.3E-43
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 4.7E-44
Sequence
Target Sequence 
>C. ELEGANS:180663_S_AT
acacgtattcacaaacacacacaaatggatctccagttaaaccatatttggttgctaata
ttgaaagaactatagatacacgacttggcgaagtatttttacataaaaaagaacggagtg
aacattgggcatcaattgcatggtttttcaatatagatgtcaaggggaaattagttgaaa
aaataataattcgtatgtcaggaattgaagagttgattcttaattctgggacaactcaaa
ttggcgaaaatttaaatttaatatcgaaatctaccagaattccaatagaacaaaatttga
cgattgacaatccccaattagaagttttctacataatggcgctctggacaataagtttgg
acgcgagagctttcaaagctgatctggaggatgggataaactcggaaagtttttcagtcg
aagtgttctggagtaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACGTATTCACAAACACACACAAA 10 111 182 Antisense
CAAACACACACAAATGGATCTCCAG 338 191 193 Antisense
GAACTATAGATACACGACTTGGCGA 267 349 248 Antisense
GACTTGGCGAAGTATTTTTACATAA 253 371 263 Antisense
GAACGGAGTGAACATTGGGCATCAA 310 349 292 Antisense
TGGGCATCAATTGCATGGTTTTTCA 305 555 307 Antisense
GAGTTGATTCTTAATTCTGGGACAA 243 397 391 Antisense
TTCTGGGACAACTCAAATTGGCGAA 699 609 405 Antisense
ATATCGAAATCTACCAGAATTCCAA 29 21 442 Antisense
ATTTGACGATTGACAATCCCCAATT 86 19 476 Antisense
TTCAGTCGAAGTGTTCTGGAGTAAG 682 293 594 Antisense
Sequence Source Affymetrix Proprietary Database