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[180660_at]

Description Y53F4B.2 /REP_DB=WormBase Gene ID /WP=CE22388 /TR=Q9NAC6 /GB=CAB61069.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 90      6grp_total > 07      4grp_total > 11      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 217
1 2 3 4 range
A 189.8
(-0.2)
P 		182.2
(-0.6)
P 		143.4
(-3)
P 		242
(3)
P 		99
B 146.8
(-3)
P 		205.1
(1.5)
P 		166.4
(-1.5)
P 		225
(3)
P 		78
C 165
(-1.8)
P 		142.7
(-2.5)
P 		123.3
(-3)
P 		340
(3)
P 		217
avr 167.2
(-1.9) 		176.7
(-1.4) 		144.4
(-3) 		269
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 171.5
(3)
P 		139.5
(0.3)
P 		144.1
(0.7)
P 		132
(-0.3)
P 		99.5
(-3)
P 		160.6
(2.1)
P 		72
B 177
(3)
P 		143.9
(0.7)
P 		132.8
(-0.1)
P 		118.2
(-1.1)
P 		100.2
(-2.4)
P 		91.6
(-3)
P 		85
C 208
(3)
P 		161.3
(0.2)
P 		130.8
(-1.6)
P 		134.7
(-1.3)
P 		111.1
(-2.7)
P 		106.5
(-3)
P 		102
avr 185.5
(3) 		148.2
(0.3) 		135.9
(-0.6) 		128.3
(-1.2) 		103.6
(-3) 		119.6
(-1.8) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 69.1
(3)
P 		39.6
(-2.3)
P 		37.5
(-2.6)
P 		35.4
(-3)
P 		34
B 46.8
(1.1)
P 		50.6
(3)
P 		38.6
(-3)
P 		47.9
(1.7)
P 		12
C 54.6
(2)
P 		41
(-3)
P 		43
(-2.3)
P 		57.3
(3)
P 		16
avr 56.8
(3) 		43.7
(-1.6) 		39.7
(-3) 		46.9
(-0.5) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 31.6
(-2.5)
P 		49.6
(3)
P 		31.4
(-2.6)
P 		33.5
(-1.9)
P 		30
(-3)
P 		39.1
(-0.2)
P 		20
B 61.6
(3)
P 		47.9
(0.3)
P 		32.2
(-2.8)
P 		31.3
(-3)
P 		40.7
(-1.1)
P 		34.2
(-2.4)
P 		30
C 77.1
(3)
P 		49.2
(-0.3)
P 		33.3
(-2.3)
P 		27.1
(-3)
P 		35.9
(-1.9)
P 		35.7
(-2)
P 		50
avr 56.8
(3) 		48.9
(1.2) 		32.3
(-2.6) 		30.6
(-3) 		35.5
(-1.9) 		36.3
(-1.7) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180660_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2776
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.2 NCBI
Target Description Y53F4B.2 /REP_DB=WormBase Gene ID /WP=CE22388 /TR=Q9NAC6 /GB=CAB61069.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064685(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064685 NCBI Caenorhabditis elegans fatty acid ELOngation family member (elo-9) (elo-9) mRNA, complete cds. 11/11 None
GENEFINDER00000043126 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14959709:14963991:-1 11/11 None
Y53F4B.2 ENSEMBL cdna:known chromosome:CEL140:II:14959709:14963991:-1 gene:Y53F4B.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043034 4/11 Cross Hyb Matching Probes None
SNAP00000043035 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14959705-14963988(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol elo-9
Ensembl Y53F4B.2
Entrez Gene 190207 Entrez gene
SwissProt Q9NAC6 EMBL-EBI
WormBase CE22388 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61069 Hypothetical protein Y53F4B.2 [Caenorhabditis elegans] ref|NP_497086.1| fatty acid ELOngation family member (elo-9) [Caenorhabditis elegans] 1.0E-153
blast CAE73448 Hypothetical protein CBG20897 [Caenorhabditis briggsae] 1.0E-147
blast CAA79555 Hypothetical protein C40H1.4 [Caenorhabditis elegans] ref|NP_499056.1| fatty acid ELOngation family member (elo-4) [Caenorhabditis elegans] sp|Q03574|ELO4_CAEEL Putative fatty acid elongation protein 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR002076 EMBL-EBI GNS1/SUR4 membrane protein 1.1E-83
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497086.1 6 48-70,80-102,155-174,184-206,215-237,252-274
Y53F4B.2 6 48-70,80-102,155-174,184-206,215-237,252-274
GENEFINDER00000043126 6 48-70,80-102,155-174,184-206,215-237,252-274
Sequence
Target Sequence 
>C. ELEGANS:180660_AT
gcgacctcttttactagcatggaacggttttttggcagtgttcagtattatgggtacatg
gagatttggaatcgaattctacgatgctgttttcagaagaggcttcatcgattcgatctg
cctggctgtaaatccacgttcaccgtccgcattctgggcatgcatgttcgctctatcgaa
aatcgccgagtttggggacacgatgttcttggtgctgaggaaacggccggttatattcct
tcactggtatcatcacgctgttgttctgatcctttcttggcatgctgcaatcgaactcac
agctccaggacgctggtttatttttatgaactatttggtgcattcaataatgtatacata
ctacgcaataacatcaatcggctatcgtcttcccaaaatcgtttcaatgactgttacatt
ccttcaaactcttcaaatgctcattggtgtcagcatttcttgcattgtgctttatttgaa
gcttaatggagagatgtgccaacaatcctacgacaatctggcgttgagcttcggaatcta
cgcctcattcctggtgctattctccagtttcttcaacaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGACCTCTTTTACTAGCATGGAAC 648 289 246 Antisense
GAGGCTTCATCGATTCGATCTGCCT 681 405 344 Antisense
GATCTGCCTGGCTGTAAATCCACGT 61 419 360 Antisense
GCATTCTGGGCATGCATGTTCGCTC 690 307 394 Antisense
CACTGGTATCATCACGCTGTTGTTC 378 199 487 Antisense
ATCCTTTCTTGGCATGCTGCAATCG 188 39 514 Antisense
AACTCACAGCTCCAGGACGCTGGTT 348 141 539 Antisense
GCTATCGTCTTCCCAAAATCGTTTC 318 303 626 Antisense
GAGATGTGCCAACAATCCTACGACA 134 397 736 Antisense
CGTTGAGCTTCGGAATCTACGCCTC 585 247 767 Antisense
GCTATTCTCCAGTTTCTTCAACAAT 90 305 801 Antisense
Sequence Source Affymetrix Proprietary Database