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[180657_at]

Description C06B3.10 /REP_DB=WormBase Gene ID /WP=CE07967 /TR=Q17709 /GB=CAB01119.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 0      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 36.4
(-2)
A 		27.3
(-3)
A 		34.7
(-2.2)
A 		79.9
(3)
A 		53
B 25.4
(-0.9)
A 		6.2
(-3)
A 		31.5
(-0.3)
A 		61.7
(3)
A 		56
C 26.7
(-2.1)
A 		25.2
(-3)
A 		35.7
(3)
A 		27.7
(-1.6)
A 		11
avr 29.5
(-1.4) 		19.6
(-3) 		34
(-0.7) 		56.4
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 59
(0.8)
A 		39.9
(-0.7)
A 		63.1
(1.1)
A 		10.7
(-3)
A 		87.1
(3)
A 		12.7
(-2.8)
A 		76
B 53.2
(3)
A 		10.9
(-3)
A 		51.6
(2.8)
A 		35.1
(0.4)
A 		22.6
(-1.3)
A 		32.3
(0)
A 		42
C 23.1
(-2.5)
A 		45.4
(0)
A 		19.1
(-3)
A 		51.9
(0.8)
A 		21.3
(-2.7)
A 		71
(3)
A 		52
avr 45.1
(3) 		32.1
(-3) 		44.6
(2.8) 		32.6
(-2.8) 		43.7
(2.4) 		38.7
(0) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 13.2
(3)
A 		5.9
(-3)
A 		9.1
(-0.4)
A 		11.7
(1.8)
A 		7
B 8.1
(0.4)
A 		1.5
(-3)
A 		7.3
(-0)
A 		13.2
(3)
A 		12
C 8.9
(0.3)
A 		7.3
(-1)
A 		12.4
(3)
A 		4.7
(-3)
A 		8
avr 10.1
(3) 		4.9
(-3) 		9.6
(2.4) 		9.9
(2.8) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 10.9
(-0.9)
A 		14.2
(-0.1)
A 		13.7
(-0.2)
A 		2.7
(-3)
A 		26.3
(3)
A 		3.1
(-2.9)
A 		24
B 18.5
(3)
A 		3.6
(-3)
A 		12.5
(0.6)
A 		9.3
(-0.7)
A 		9.2
(-0.7)
A 		12
(0.4)
A 		15
C 8.6
(-1.8)
A 		13.9
(-0.1)
A 		4.9
(-3)
A 		10.4
(-1.3)
A 		6.9
(-2.4)
A 		23.8
(3)
A 		19
avr 12.7
(1.7) 		10.6
(-0.2) 		10.4
(-0.4) 		7.5
(-3) 		14.1
(3) 		13
(2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180657_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14219
Sequence Type Exemplar sequence
Representative Public ID C06B3.10 NCBI
Target Description C06B3.10 /REP_DB=WormBase Gene ID /WP=CE07967 /TR=Q17709 /GB=CAB01119.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074053(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074053 NCBI Caenorhabditis elegans Seven TM Receptor family member (str-250) (str-250) mRNA, complete cds. 11/11 None
GENEFINDER00000008257 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13921104:13922870:1 11/11 None
C06B3.10 ENSEMBL cdna:known chromosome:CEL140:V:13921230:13922621:1 gene:C06B3.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13921229-13922621(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol str-250
Ensembl C06B3.10
Entrez Gene 182295 Entrez gene
SwissProt Q17709 EMBL-EBI
WormBase CE07967 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01119 Hypothetical protein C06B3.10 [Caenorhabditis elegans] ref|NP_506454.1| Seven TM Receptor family member (str-250) [Caenorhabditis elegans] 1.0E-176
blast CAB01119 Hypothetical protein C06B3.10 [Caenorhabditis elegans] ref|NP_506454.1| Seven TM Receptor family member (str-250) [Caenorhabditis elegans] 1.0E-170
blast CAB01122 Hypothetical protein C06B3.9 [Caenorhabditis elegans] ref|NP_506453.1| Seven TM Receptor family member (str-252) [Caenorhabditis elegans] 6.0E-67
blast CAB01122 Hypothetical protein C06B3.9 [Caenorhabditis elegans] ref|NP_506453.1| Seven TM Receptor family member (str-252) [Caenorhabditis elegans] 8.0E-67
blast CAE66196 Hypothetical protein CBG11436 [Caenorhabditis briggsae] 7.0E-63
blast CAE66196 Hypothetical protein CBG11436 [Caenorhabditis briggsae] 1.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 3.2E-8
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 3.2E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506454.1 7 5-27,40-59,84-106,127-149,164-183,196-218,245-267
C06B3.10 7 5-27,40-59,84-106,127-149,164-183,196-218,245-267
GENEFINDER00000008257 7 47-69,82-101,126-148,169-191,206-225,238-260,287-309
Sequence
Target Sequence 
>C. ELEGANS:180657_AT
acatttcctgttatcttcgggttggtgtggttttggatgactcacttcaccctcacagca
tctgacgaaacggagcaatttttcaacaagtattattacggtgttcacaatagtgcgtca
gatattttctacgtgggatattatttctacccagaagatgcatatggaaacaaggcatta
cactcacgatcttttagaggatttttattccaatcaacttttataacttcctcatttgga
ttaatactctactttggaacaaaatgtcatcttcacacaaaatcattatcctccaaaact
gtcacttcttcttcacgtttctctcttcaatcacaattattctatgcactggtgtttcaa
actatcattcctgtatttcttatgcatattccgtcaactatcagcatgtgtgccgcattc
acaaacatatcaattgagttcatcagtcaaatcctttcctgcacaatatctttgtacccg
gcacttgatcctcttcccaactttttcataatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATTTCCTGTTATCTTCGGGTTGG 537 103 409 Antisense
GGTTTTGGATGACTCACTTCACCCT 490 505 437 Antisense
CACCCTCACAGCATCTGACGAAACG 226 201 456 Antisense
GGCATTACACTCACGATCTTTTAGA 418 537 582 Antisense
TTATCCTCCAAAACTGTCACTTCTT 320 681 694 Antisense
CGTTTCTCTCTTCAATCACAATTAT 351 247 724 Antisense
ATGCATATTCCGTCAACTATCAGCA 354 43 790 Antisense
CTATCAGCATGTGTGCCGCATTCAC 388 217 806 Antisense
TCATCAGTCAAATCCTTTCCTGCAC 36 621 848 Antisense
GCACAATATCTTTGTACCCGGCACT 696 317 869 Antisense
CTCTTCCCAACTTTTTCATAATCAA 405 229 899 Antisense
Sequence Source Affymetrix Proprietary Database