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[180654_at]

Description T07A9.5 /REP_DB=WormBase Gene ID /WP=CE17214 /TR=O44406 /GB=AAK39278.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_high_8_8 > 48      6grp_highest_nonorm > 92      6grp_total > 87      4grp_total > 63      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 277.9
(3)
P 		157.7
(-3)
P 		232.3
(0.7)
P 		188.7
(-1.5)
A 		120
B 263.3
(3)
P 		203.2
(-1.5)
P 		195.8
(-2)
P 		182.7
(-3)
P 		81
C 271
(3)
P 		233.6
(-0)
P 		238.2
(0.3)
P 		196.9
(-3)
P 		74
avr 270.7
(3) 		198.2
(-2.4) 		222.1
(-0.6) 		189.4
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 266.9
(-1.1)
P 		259.7
(-1.4)
P 		208.7
(-3)
P 		287.9
(-0.5)
P 		266.1
(-1.2)
P 		397.3
(3)
P 		189
B 224.7
(-2.8)
P 		249.6
(-1.5)
P 		221.3
(-3)
P 		242.5
(-1.8)
P 		288.8
(0.7)
P 		331.2
(3)
P 		110
C 193.1
(-3)
P 		264.5
(-1.3)
P 		258.9
(-1.4)
P 		264.9
(-1.3)
P 		348.8
(0.7)
P 		446.8
(3)
P 		254
avr 228.2
(-3) 		257.9
(-1.9) 		229.6
(-2.9) 		265.1
(-1.6) 		301.2
(-0.3) 		391.8
(3) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 101.2
(3)
P 		34.2
(-2.5)
P 		60.7
(-0.3)
P 		27.6
(-3)
A 		74
B 84
(3)
P 		50.2
(-1.5)
P 		45.4
(-2.1)
P 		38.9
(-3)
P 		45
C 89.7
(3)
P 		67.2
(0.6)
P 		83
(2.3)
P 		33.2
(-3)
P 		57
avr 91.6
(3) 		50.5
(-1.2) 		63
(0.1) 		33.2
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 49.2
(-2.6)
P 		92.4
(2.5)
P 		45.4
(-3)
P 		73
(0.2)
P 		80.3
(1.1)
P 		96.7
(3)
P 		51
B 78.2
(-0.9)
P 		83.1
(-0.5)
P 		53.7
(-3)
P 		64.2
(-2.1)
P 		117.4
(2.5)
P 		123.5
(3)
P 		70
C 71.6
(-1.9)
P 		80.7
(-1.3)
P 		65.9
(-2.2)
P 		53.3
(-3)
P 		112.9
(0.7)
P 		149.6
(3)
P 		96
avr 66.3
(-2) 		85.4
(-0.3) 		55
(-3) 		63.5
(-2.3) 		103.5
(1.3) 		123.3
(3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180654_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11143
Sequence Type Exemplar sequence
Representative Public ID T07A9.5 NCBI
Target Description T07A9.5 /REP_DB=WormBase Gene ID /WP=CE17214 /TR=O44406 /GB=AAK39278.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171244(11), NM_171245(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171244 NCBI Caenorhabditis elegans Enhanced RNAI (RNA interference) family member (eri-1) (eri-1) mRNA, complete cds. 11/11 A 
NM_171245 NCBI Caenorhabditis elegans Enhanced RNAI (RNA interference) family member (eri-1) (eri-1) mRNA, complete cds. 11/11 None
SNAP00000017767 ENSEMBL cdna:SNAP chromosome:CEL140:IV:415777:419951:-1 11/11 None
GENEFINDER00000017774 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:415777:419951:-1 11/11 None
T07A9.5b ENSEMBL cdna:known chromosome:CEL140:IV:415654:419951:-1 gene:T07A9.5 11/11 A 
T07A9.5a ENSEMBL cdna:known chromosome:CEL140:IV:416868:419958:-1 gene:T07A9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:417080-419951(-) 100.0 100.0
Public Domain and Genome References
Ensembl T07A9.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39277 Enhanced rnai (rna interference) protein 1, isoform b [Caenorhabditis elegans] ref|NP_741292.1| Enhanced RNAI (RNA interference) family member (eri-1) [Caenorhabditis elegans] sp|O44406|ERI1_CAEEL 3'-5' exonuclease eri-1 (Enhanced RNAi protein) 0.0
blast AAK39278 Enhanced rnai (rna interference) protein 1, isoform a [Caenorhabditis elegans] ref|NP_741293.1| Enhanced RNAI (RNA interference) family member (eri-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006055 EMBL-EBI Exonuclease 1.2E-46
Pfam IPR003034 EMBL-EBI DNA-binding SAP 1.9E-8
Sequence
Target Sequence 
>C. ELEGANS:180654_AT
gttactgatggacctcacgatatgtggaaattcatgcaatttcaatgccttttatcgaat
attcgaatgccccatatgttccgaagttttatcaacatcaaaaagacattcaaagagaaa
ttcaatggacttatcaagggaaatggaaaatccggaattgaaaacatgttagaacgtctg
gatctgtcgtttgttggaaataaacatagtggattggatgatgcaacgaatattgcagcg
attgcgattcaaatgatgaagttgaaaattgagttgagaattaatcagaaatgctcgtat
aaggagaatcaacggagtgctgcgcgaaaggatgaagagcgagagttagaagacgctgca
aacgtggatcttacatcagtcgatatctctcgtcgtgattttcaactttggatgcgtcgt
ttaccactgaaactgtcctctgtgacacgacgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTACTGATGGACCTCACGATATGT 695 445 757 Antisense
GCAATTTCAATGCCTTTTATCGAAT 172 323 792 Antisense
TATTCGAATGCCCCATATGTTCCGA 641 663 816 Antisense
CGTCTGGATCTGTCGTTTGTTGGAA 133 247 931 Antisense
GGAGAATCAACGGAGTGCTGCGCGA 564 519 1059 Antisense
CGCTGCAAACGTGGATCTTACATCA 702 253 1110 Antisense
ACATCAGTCGATATCTCTCGTCGTG 705 105 1129 Antisense
ATCTCTCGTCGTGATTTTCAACTTT 652 31 1141 Antisense
TTCAACTTTGGATGCGTCGTTTACC 405 659 1157 Antisense
GTCGTTTACCACTGAAACTGTCCTC 312 473 1172 Antisense
AACTGTCCTCTGTGACACGACGAGA 409 143 1187 Antisense
Sequence Source Affymetrix Proprietary Database