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[180648_at]

Description T20D4.5 /REP_DB=WormBase Gene ID /WP=CE13788 /TR=P91470 /GB=AAB37592.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 41      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 29.9
(-2.2)
A 		82.4
(3)
A 		66.3
(1.4)
P 		21.9
(-3)
A 		61
B 29
(-3)
A 		73.5
(3)
A 		44.9
(-0.9)
P 		51.6
(0)
A 		45
C 19
(-3)
A 		45.2
(1)
A 		58.6
(3)
A 		56.8
(2.7)
A 		40
avr 26
(-3) 		67
(3) 		56.6
(1.5) 		43.4
(-0.5) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 21.6
(-1.5)
A 		67.2
(3)
P 		36.7
(-0)
P 		45.9
(0.9)
A 		33.2
(-0.4)
A 		6.6
(-3)
A 		61
B 17.5
(-2.6)
A 		52.8
(3)
A 		16.2
(-2.8)
A 		14.7
(-3)
A 		26.6
(-1.1)
A 		42
(1.3)
A 		38
C 47.8
(1.1)
P 		51.9
(1.5)
A 		38.7
(0.1)
A 		16.3
(-2.4)
A 		65.1
(3)
M 		10.6
(-3)
A 		55
avr 29
(-1.5) 		57.3
(3) 		30.5
(-1.3) 		25.6
(-2.1) 		41.6
(0.5) 		19.7
(-3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10.9
(0.1)
A 		17.9
(3)
A 		17.3
(2.8)
P 		3.2
(-3)
A 		15
B 9.2
(-3)
A 		18.1
(3)
A 		10.4
(-2.2)
P 		11
(-1.8)
A 		9
C 6.3
(-3)
A 		13
(-0.1)
A 		20.4
(3)
A 		9.6
(-1.6)
A 		14
avr 8.8
(-2.4) 		16.3
(3) 		16
(2.8) 		7.9
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 4
(-2.4)
A 		23.9
(3)
P 		8
(-1.3)
P 		11.6
(-0.3)
A 		10
(-0.7)
A 		1.6
(-3)
A 		22
B 6.1
(-2)
A 		17.6
(3)
A 		3.9
(-3)
A 		3.9
(-3)
A 		10.8
(0)
A 		15.7
(2.2)
A 		14
C 17.7
(1.9)
P 		15.8
(1.2)
A 		9.8
(-0.8)
A 		3.3
(-3)
A 		21.1
(3)
M 		3.6
(-2.9)
A 		18
avr 9.3
(-1.6) 		19.1
(3) 		7.2
(-2.6) 		6.3
(-3) 		14
(0.6) 		7
(-2.7) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15493
Sequence Type Exemplar sequence
Representative Public ID T20D4.5 NCBI
Target Description T20D4.5 /REP_DB=WormBase Gene ID /WP=CE13788 /TR=P91470 /GB=AAB37592.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071555(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071555 NCBI Caenorhabditis elegans T20D4.5 (T20D4.5) mRNA, complete cds. 10/11 None
SNAP00000008851 ENSEMBL cdna:SNAP chromosome:CEL140:V:3413170:3415533:1 11/11 None
GENEFINDER00000008859 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3413170:3422452:1 9/11 None
T20D4.5 ENSEMBL cdna:known chromosome:CEL140:V:3413170:3415642:1 gene:T20D4.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3413169-3415533(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.5
Entrez Gene 188641 Entrez gene
SwissProt P91470 EMBL-EBI
WormBase CE35778 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37592 Hypothetical protein T20D4.5 [Caenorhabditis elegans] ref|NP_503956.2| T20D4.5 [Caenorhabditis elegans] 0.0
blast AAB37594 Hypothetical protein T20D4.3 [Caenorhabditis elegans] ref|NP_503958.2| T20D4.3 [Caenorhabditis elegans] 0.0
blast AAB37594 Hypothetical protein T20D4.3 [Caenorhabditis elegans] ref|NP_503958.2| T20D4.3 [Caenorhabditis elegans] 1.0E-172
blast AAB37594 Hypothetical protein T20D4.3 [Caenorhabditis elegans] ref|NP_503958.2| T20D4.3 [Caenorhabditis elegans] 1.0E-161
blast AAB37593 Hypothetical protein T20D4.4 [Caenorhabditis elegans] ref|NP_503957.2| T20D4.4 [Caenorhabditis elegans] 1.0E-111
blast AAC27091 Hypothetical protein C04E12.5 [Caenorhabditis elegans] ref|NP_503935.2| C04E12.5 [Caenorhabditis elegans] 2.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 7.9E-46
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.8E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 3.6E-44
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.5E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.3E-43
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 4.7E-44
Sequence
Target Sequence 
>C. ELEGANS:180648_AT
gcagtggggtaacgttttcaatcgaagtttactggaaaacagtcaattttccaacaccag
tatcttccaatacgactacgtctgaaaagttctctacatcttcaagatcgaacgatacgg
aactacttcgtcgttctgaagaacttaaagaacagagcgaatatattcaattgcaaccag
agtcaggacaaaatcatgtcaagttcacttacgacattatcaacaacatttattcacatg
ataaccaggatgggtcaccagttaacccgaaacaagtctttaacattaaacgagtagtag
acgaggaaaacaatgaggcatacataggtcaggaagccccttatagacggtcaaatatct
actggatgttcgatttaaggaacaaaggaagaaatgttgaaaaagtagtggtcaaaatga
ccggaatggacgatattacccaacaccgagcaggaactgttgaatgctgtttaaactatt
tgatagtctgcaaatctgacaaacttactcgattcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGTGGGGTAACGTTTTCAATCGA 190 309 686 Antisense
AATTTTCCAACACCAGTATCTTCCA 526 175 730 Antisense
GTATCTTCCAATACGACTACGTCTG 475 453 745 Antisense
AAAGTTCTCTACATCTTCAAGATCG 178 123 771 Antisense
GATACGGAACTACTTCGTCGTTCTG 40 423 799 Antisense
CCAGGATGGGTCACCAGTTAACCCG 44 261 930 Antisense
GGTCAGGAAGCCCCTTATAGACGGT 60 253 1012 Antisense
GACGGTCAAATATCTACTGGATGTT 197 377 1031 Antisense
GAATGGACGATATTACCCAACACCG 531 331 1109 Antisense
ATTACCCAACACCGAGCAGGAACTG 482 13 1120 Antisense
ATCTGACAAACTTACTCGATTCATT 660 33 1179 Antisense
Sequence Source Affymetrix Proprietary Database