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[180636_at]

Description T22B2.5 /REP_DB=WormBase Gene ID /WP=CE13936 /TR=O45191 /GB=AAC04443.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 3.1
(-3)
A 		4.7
(-2.9)
A 		2.9
(-3)
A 		94.6
(3)
P 		92
B 3.1
(-1.3)
A 		4.6
(1.9)
A 		2.3
(-3)
A 		5.1
(3)
A 		3
C 3.4
(-2.8)
A 		4.6
(-2.5)
A 		2
(-3)
A 		36.2
(3)
P 		34
avr 3.2
(-2.9) 		4.6
(-2.7) 		2.4
(-3) 		45.3
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 40.8
(3)
A 		1.1
(-3)
A 		23.1
(0.3)
A 		2.4
(-2.8)
A 		35.9
(2.3)
A 		3.4
(-2.7)
A 		40
B 1.3
(-2.7)
A 		1.2
(-2.8)
A 		2.4
(-0.8)
A 		4.7
(3)
A 		3.4
(0.8)
A 		1.1
(-3)
A 		4
C 0.8
(-3)
A 		27
(3)
A 		1.8
(-2.8)
A 		3.6
(-2.4)
A 		2.5
(-2.6)
A 		10.9
(-0.7)
A 		26
avr 14.3
(3) 		9.8
(0.5) 		9.1
(0.1) 		3.6
(-3) 		13.9
(2.8) 		5.1
(-2.2) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.1
(-2.9)
A 		1
(-2.9)
A 		0.8
(-3)
A 		13.8
(3)
P 		13
B 1
(2)
A 		1.1
(3)
A 		0.5
(-3)
A 		1.1
(3)
A 		1
C 1.1
(-2.6)
A 		1.3
(-2.3)
A 		0.7
(-3)
A 		6.1
(3)
P 		5
avr 1.1
(-2.6) 		1.1
(-2.6) 		0.7
(-3) 		7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 7.5
(1.1)
A 		0.4
(-3)
A 		5
(-0.3)
A 		0.6
(-2.9)
A 		10.8
(3)
A 		0.8
(-2.8)
A 		10
B 0.5
(-2.4)
A 		0.4
(-3)
A 		0.6
(-1.8)
A 		1.2
(1.8)
A 		1.4
(3)
A 		0.4
(-3)
A 		1
C 0.3
(-3)
A 		8.2
(3)
A 		0.5
(-2.8)
A 		0.7
(-2.7)
A 		0.8
(-2.6)
A 		3.6
(-0.5)
A 		8
avr 2.8
(0.4) 		3
(0.8) 		2
(-0.9) 		0.8
(-3) 		4.3
(3) 		1.6
(-1.6) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180636_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18368
Sequence Type Exemplar sequence
Representative Public ID T22B2.5 NCBI
Target Description T22B2.5 /REP_DB=WormBase Gene ID /WP=CE13936 /TR=O45191 /GB=AAC04443.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076275(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076275 NCBI Caenorhabditis elegans T22B2.5 (T22B2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000014691 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3907975:3911131:-1 11/11 None
T22B2.5 ENSEMBL cdna:known chromosome:CEL140:X:3909806:3911359:-1 gene:T22B2.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014682 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3909806-3911360(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22B2.5
Entrez Gene 188719 Entrez gene
SwissProt O45191 EMBL-EBI
WormBase CE35781 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC04443 Hypothetical protein T22B2.5 [Caenorhabditis elegans] ref|NP_508676.2| T22B2.5 [Caenorhabditis elegans] 1.0E-136
blast AAC04443 Hypothetical protein T22B2.5 [Caenorhabditis elegans] ref|NP_508676.2| T22B2.5 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508676.2 5 26-48,69-86,101-123,130-152,222-244
T22B2.5 5 26-48,69-86,101-123,130-152,222-244
GENEFINDER00000014691 4 7-29,44-66,73-95,165-187
Sequence
Target Sequence 
>C. ELEGANS:180636_AT
gatgtggcttaacttggctggtttaattatgaccatttttccagataccgcgaaatcaga
tatcaaatttgttgagaatgttgttctattgaacattttgatatactctgtagtgacatc
aatcggaggattaggcgtttgtgaaattaacctacgaagtaaaagagaaaaatacttccg
agtcatcttctggatttttatcgtcttcattcatctatgtcaggctgtttatgtatctct
atctccaatgaaaaatgcaacattggttgcttctgcatgttactctgccaatgtcttgaa
tgatttgaaatctcttccaattggtgaattagctaaacaatcgactatactctgttcaga
acttcgtggaaccgaagagatggaatctgttatggtggaaccgagacgatctagcatttt
cgacgattcatcagaacatgaagttgatattctatatgcccgactttattatagtctctt
gatgttgtccgttgcattttccacttatcacattttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTGGCTTAACTTGGCTGGTTTA 448 407 285 Antisense
TGACCATTTTTCCAGATACCGCGAA 147 571 314 Antisense
AATACTTCCGAGTCATCTTCTGGAT 668 171 455 Antisense
GGATTTTTATCGTCTTCATTCATCT 265 509 476 Antisense
GGCTGTTTATGTATCTCTATCTCCA 266 541 507 Antisense
TTGCTTCTGCATGTTACTCTGCCAA 483 703 551 Antisense
CAATCGACTATACTCTGTTCAGAAC 510 181 622 Antisense
GACGATCTAGCATTTTCGACGATTC 470 377 689 Antisense
GATATTCTATATGCCCGACTTTATT 303 427 730 Antisense
TTATAGTCTCTTGATGTTGTCCGTT 463 679 753 Antisense
TGCATTTTCCACTTATCACATTTTT 565 577 777 Antisense
Sequence Source Affymetrix Proprietary Database