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[180633_at]

Description C53B7.7 /REP_DB=WormBase Gene ID /WP=CE17602 /TR=O45311 /GB=AAC48280.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 02      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 2.6
(-3)
A 		2.7
(-3)
A 		2.9
(-2.9)
A 		16.5
(3)
A 		14
B 4.7
(-1.5)
A 		4.7
(-1.5)
A 		2.9
(-3)
A 		10.1
(3)
A 		7
C 3.5
(-2.8)
A 		4
(-2.7)
A 		1.9
(-3)
A 		49.9
(3)
A 		48
avr 3.6
(-2.7) 		3.8
(-2.7) 		2.6
(-3) 		25.5
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 5.3
(-2.3)
A 		1.3
(-3)
A 		20.4
(0.1)
A 		8.9
(-1.8)
A 		38.1
(3)
A 		8.2
(-1.9)
A 		37
B 2.9
(-2.6)
A 		6.1
(-1.5)
A 		3.4
(-2.5)
A 		1.9
(-3)
A 		18.8
(3)
A 		6.3
(-1.4)
A 		17
C 2.3
(-3)
A 		2.6
(-2.9)
A 		1.9
(-3)
A 		4.5
(-2.7)
A 		53.7
(3)
A 		46.9
(2.2)
A 		52
avr 3.5
(-3) 		3.3
(-3) 		8.6
(-2.1) 		5.1
(-2.7) 		36.9
(3) 		20.5
(0.1) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 1
(-1.7)
A 		0.6
(-3)
A 		0.8
(-2.3)
A 		2.4
(3)
A 		2
B 1.5
(0.2)
A 		1.2
(-1)
A 		0.7
(-3)
A 		2.2
(3)
A 		2
C 1.2
(-2.6)
A 		1.2
(-2.6)
A 		0.7
(-3)
A 		8.4
(3)
A 		8
avr 1.2
(-2.2) 		1
(-2.5) 		0.7
(-3) 		4.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 1
(-2.7)
A 		0.5
(-3)
A 		4.4
(-0.9)
A 		2.3
(-2)
A 		11.5
(3)
A 		2
(-2.2)
A 		11
B 1
(-2.6)
A 		2
(-1.7)
A 		0.8
(-2.7)
A 		0.5
(-3)
A 		7.6
(3)
A 		2.3
(-1.5)
A 		7
C 0.9
(-2.9)
A 		0.8
(-2.9)
A 		0.5
(-3)
A 		0.9
(-2.9)
A 		17.4
(3)
A 		15.7
(2.4)
A 		17
avr 1
(-3) 		1.1
(-2.9) 		1.9
(-2.5) 		1.2
(-2.9) 		12.2
(3) 		6.7
(0.1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180633_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16579
Sequence Type Exemplar sequence
Representative Public ID C53B7.7 NCBI
Target Description C53B7.7 /REP_DB=WormBase Gene ID /WP=CE17602 /TR=O45311 /GB=AAC48280.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076755 NCBI Caenorhabditis elegans C53B7.7 (C53B7.7) mRNA, complete cds. 11/11 None
SNAP00000013981 ENSEMBL cdna:SNAP chromosome:CEL140:X:6861042:6862432:-1 9/11 None
C53B7.7 ENSEMBL cdna:known chromosome:CEL140:X:6861042:6863366:-1 gene:C53B7.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000013983 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6861041-6863366(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C53B7.7
Entrez Gene 183746 Entrez gene
SwissProt O45311 EMBL-EBI
WormBase CE35438 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC48280 Hypothetical protein C53B7.7 [Caenorhabditis elegans] ref|NP_509156.2| C53B7.7 [Caenorhabditis elegans] 0.0
blast AAC48280 Hypothetical protein C53B7.7 [Caenorhabditis elegans] ref|NP_509156.2| C53B7.7 [Caenorhabditis elegans] 1.0E-166
blast CAE65809 Hypothetical protein CBG10917 [Caenorhabditis briggsae] 2.0E-43
blast AAA64336 Hypothetical protein F40B5.1 [Caenorhabditis elegans] ref|NP_509420.2| F40B5.1 [Caenorhabditis elegans] sp|Q09319|YQZ1_CAEEL Hypothetical protein F40B5.1 in chromosome X 4.0E-42
blast CAE65803 Hypothetical protein CBG10911 [Caenorhabditis briggsae] 4.0E-40
blast CAE65809 Hypothetical protein CBG10917 [Caenorhabditis briggsae] 1.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 4.2E-83
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.2E-45
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.2E-17
Sequence
Target Sequence 
>C. ELEGANS:180633_AT
aatcggccatactttcattgaacacagtatcaatcctgacggtcttccgtatttttcaaa
gccagctgaggattgcgttcagaatcaatatttaaagacttgcaatgaatattacgaagg
ggaagttcctgatgcttgcaatacttcagactatacatttgatgataatggtgctgatgt
ttttggtctgcaacttgcatatgcaatactggagagagatttgagcgaccaattgagaga
gcaagctgatggtttaaaaatcacaaacgaacaattgttgttctattcatttgcctacag
attttgcaggggatcaaaatccaatacaacagatggctcgcattcgcatcacaatgttcg
aattaatgctgttgcacaaatgccaggatttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGGCCATACTTTCATTGAACAC 344 169 825 Antisense
AGTATCAATCCTGACGGTCTTCCGT 20 63 850 Antisense
GGTCTTCCGTATTTTTCAAAGCCAG 198 501 865 Antisense
CAAAGCCAGCTGAGGATTGCGTTCA 94 193 881 Antisense
AGGGGAAGTTCCTGATGCTTGCAAT 513 55 942 Antisense
GATGCTTGCAATACTTCAGACTATA 592 413 955 Antisense
GATGTTTTTGGTCTGCAACTTGCAT 66 409 1000 Antisense
GTTGTTCTATTCATTTGCCTACAGA 429 437 1101 Antisense
ATACAACAGATGGCTCGCATTCGCA 462 23 1148 Antisense
GCTCGCATTCGCATCACAATGTTCG 571 299 1160 Antisense
GCTGTTGCACAAATGCCAGGATTTC 658 295 1192 Antisense
Sequence Source Affymetrix Proprietary Database