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[180629_at]

Description T28B4.2 /REP_DB=WormBase Gene ID /WP=CE14323 /TR=O17343 /GB=AAK39306.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 12.4
(3)
A 		5.1
(-2.8)
A 		4.9
(-3)
A 		5.8
(-2.3)
A 		8
B 29
(2.2)
A 		5.3
(-3)
A 		32.8
(3)
A 		30.6
(2.5)
A 		28
C 45.1
(2)
A 		9.6
(-3)
A 		29.5
(-0.2)
A 		52.1
(3)
A 		43
avr 28.8
(2.8) 		6.7
(-3) 		22.4
(1.1) 		29.5
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 19.1
(-0.5)
A 		5.8
(-2.8)
A 		32.5
(1.8)
A 		5.2
(-2.9)
A 		39.7
(3)
A 		4.6
(-3)
A 		35
B 41.4
(3)
A 		5.4
(-2.6)
A 		24.6
(0.4)
A 		3.1
(-3)
A 		4.5
(-2.8)
A 		23.6
(0.2)
A 		38
C 43.4
(3)
A 		3.2
(-3)
A 		12.4
(-1.6)
A 		20.6
(-0.4)
A 		3.1
(-3)
A 		3.8
(-2.9)
A 		40
avr 34.6
(3) 		4.8
(-3) 		23.2
(0.7) 		9.6
(-2) 		15.8
(-0.8) 		10.7
(-1.8) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 4.5
(3)
A 		1.1
(-2.5)
A 		1.3
(-2.2)
A 		0.8
(-3)
A 		4
B 9.2
(3)
A 		1.3
(-3)
A 		7.6
(1.8)
A 		6.5
(0.9)
A 		8
C 14.9
(3)
A 		2.8
(-3)
A 		10.3
(0.7)
A 		8.8
(-0)
A 		12
avr 9.5
(3) 		1.7
(-3) 		6.4
(0.6) 		5.4
(-0.2) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 3.5
(-1.7)
A 		2.1
(-2.4)
A 		7.1
(0.3)
A 		1.3
(-2.9)
A 		12
(3)
A 		1.1
(-3)
A 		11
B 14.4
(3)
A 		1.8
(-2.6)
A 		6
(-0.7)
A 		0.8
(-3)
A 		1.8
(-2.6)
A 		8.8
(0.5)
A 		14
C 16.1
(3)
A 		1
(-3)
A 		3.2
(-2.1)
A 		4.1
(-1.8)
A 		1
(-3)
A 		1.3
(-2.9)
A 		15
avr 11.3
(3) 		1.6
(-3) 		5.4
(-0.6) 		2.1
(-2.7) 		4.9
(-1) 		3.7
(-1.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180629_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17242
Sequence Type Exemplar sequence
Representative Public ID T28B4.2 NCBI
Target Description T28B4.2 /REP_DB=WormBase Gene ID /WP=CE14323 /TR=O17343 /GB=AAK39306.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076706(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076706 NCBI Caenorhabditis elegans T28B4.2 (T28B4.2) mRNA, complete cds. 11/11 None
GENEFINDER00000042986 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6581959:6586025:-1 11/11 None
T28B4.2 ENSEMBL cdna:known chromosome:CEL140:X:6581959:6583544:-1 gene:T28B4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042978 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6581958-6583544(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28B4.2
Entrez Gene 189027 Entrez gene
SwissProt O17343 EMBL-EBI
WormBase CE14323 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39306 Hypothetical protein T28B4.2 [Caenorhabditis elegans] ref|NP_509107.1| T28B4.2 [Caenorhabditis elegans] 1.0E-153
blast AAK39306 Hypothetical protein T28B4.2 [Caenorhabditis elegans] ref|NP_509107.1| T28B4.2 [Caenorhabditis elegans] 1.0E-152
blast CAE65755 Hypothetical protein CBG10843 [Caenorhabditis briggsae] 1.0E-75
blast CAE65755 Hypothetical protein CBG10843 [Caenorhabditis briggsae] 3.0E-73
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509107.1 2 10-29,50-72
T28B4.2 2 10-29,50-72
GENEFINDER00000042986 2 45-67,88-110
Sequence
Target Sequence 
>C. ELEGANS:180629_AT
gactgactcgtttttcaatctcttctgcgatgtctatgaaatcagcttacactatacaac
cgagccgactaacgcagaattctttttctctttgagaaactgtaaaactgtgatgcgcac
catttactatgttatctctccatttcaactgctcgccctttgctctttgaatcctcattt
ccaaagctccatgataagatatctaaacggaacggaaacctttaatgtagttactctcaa
aacaattggaggagatacacgaacaaaagttctttatgaagatcttccggacccatcgga
actccgatggttgtcctggatgtactttagatggtgccgatgtcacatcgctgtaatagt
attccacaaatttttgaacctcgtcgaagaaagtcacaaaagtattaaatatgccagacg
acagagaaagttgaataaggaaatcgatagagataaaaaagaatcatcacagcaatcaac
tggtcactcatctcgaaaacgggaatctagagttcaaattgacgatgaaaacatgcagca
cgttgatctggtaaccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTGACTCGTTTTTCAATCTCTTC 292 375 204 Antisense
TCAATCTCTTCTGCGATGTCTATGA 503 633 218 Antisense
AAATCAGCTTACACTATACAACCGA 320 117 242 Antisense
ATACAACCGAGCCGACTAACGCAGA 688 19 257 Antisense
GATGCGCACCATTTACTATGTTATC 344 415 315 Antisense
TATGTTATCTCTCCATTTCAACTGC 29 657 331 Antisense
TTGAATCCTCATTTCCAAAGCTCCA 165 705 370 Antisense
TCGGAACTCCGATGGTTGTCCTGGA 297 605 499 Antisense
GATGGTGCCGATGTCACATCGCTGT 4 409 533 Antisense
AATCAACTGGTCACTCATCTCGAAA 602 171 677 Antisense
GCAGCACGTTGATCTGGTAACCGAC 224 313 738 Antisense
Sequence Source Affymetrix Proprietary Database