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[180628_at]

Description C53B7.3 /REP_DB=WormBase Gene ID /WP=CE06973 /TR=Q18804 /GB=AAC48274.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 25      6grp_total > 32      4grp_total > 38      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4415
1 2 3 4 range
A 6266.5
(3)
P 		3061.4
(-1.4)
P 		1851.9
(-3)
P 		4133.6
(0.1)
P 		4415
B 5970.6
(3)
P 		2957.5
(-1.6)
P 		2013.5
(-3)
P 		3954.7
(-0.1)
P 		3957
C 6177.3
(3)
P 		3024.7
(-1.4)
P 		1880.4
(-3)
P 		3112.5
(-1.3)
P 		4297
avr 6138.1
(3) 		3014.5
(-1.4) 		1915.3
(-3) 		3733.6
(-0.4) 		4223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4842
1 2 3 4 5 6 range
A 5524.3
(2.4)
P 		4711.6
(0.8)
P 		5842.6
(3)
P 		4909.5
(1.2)
P 		2762
(-3)
P 		2998.7
(-2.5)
P 		3081
B 4488.6
(1.3)
P 		5079.6
(2.3)
P 		5497.3
(3)
P 		4069.3
(0.6)
P 		2618.2
(-1.9)
P 		1971.2
(-3)
P 		3526
C 5874.5
(1.9)
P 		4862.4
(0.7)
P 		6754.4
(3)
P 		4564.1
(0.3)
P 		2937.2
(-1.7)
P 		1912.7
(-3)
P 		4842
avr 5295.8
(1.8) 		4884.5
(1.2) 		6031.4
(3) 		4514.3
(0.6) 		2772.5
(-2.2) 		2294.2
(-3) 		3737
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1815
1 2 3 4 range
A 2281.6
(3)
P 		664.5
(-2.4)
P 		484.1
(-3)
P 		605.1
(-2.6)
P 		1798
B 1904
(3)
P 		730.4
(-1.9)
P 		466.6
(-3)
P 		842.7
(-1.4)
P 		1437
C 2044.9
(3)
P 		869.6
(-1.6)
P 		655.4
(-2.5)
P 		524.8
(-3)
P 		1520
avr 2076.8
(3) 		754.8
(-2.1) 		535.4
(-3) 		657.5
(-2.5) 		1541

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1537
1 2 3 4 5 6 range
A 1018.4
(-1.2)
P 		1675.7
(3)
P 		1272.4
(0.4)
P 		1245.2
(0.3)
P 		833.4
(-2.3)
P 		730.1
(-3)
P 		946
B 1561.8
(2.2)
P 		1690.8
(3)
P 		1334.9
(0.8)
P 		1077.8
(-0.8)
P 		1063.7
(-0.9)
P 		735.1
(-3)
P 		956
C 2177.4
(3)
P 		1483.7
(0.3)
P 		1719.1
(1.2)
P 		917.8
(-1.9)
P 		950.4
(-1.8)
P 		640.3
(-3)
P 		1537
avr 1585.9
(2.8) 		1616.7
(3) 		1442.1
(1.9) 		1080.3
(-0.5) 		949.2
(-1.4) 		701.8
(-3) 		915
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180628_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16189
Sequence Type Exemplar sequence
Representative Public ID C53B7.3 NCBI
Target Description C53B7.3 /REP_DB=WormBase Gene ID /WP=CE06973 /TR=Q18804 /GB=AAC48274.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076752(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076752 NCBI Caenorhabditis elegans C53B7.3 (C53B7.3) mRNA, complete cds. 11/11 A 
SNAP00000013978 ENSEMBL cdna:SNAP chromosome:CEL140:X:6844628:6845865:1 11/11 None
GENEFINDER00000013987 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6844721:6845852:1 11/11 None
C53B7.3 ENSEMBL cdna:known chromosome:CEL140:X:6844707:6845922:1 gene:C53B7.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6844720-6845852(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C53B7.3
Entrez Gene 180957 Entrez gene
SwissProt Q18804 EMBL-EBI
WormBase CE06973 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48274 Hypothetical protein C53B7.3 [Caenorhabditis elegans] ref|NP_509153.1| C53B7.3 [Caenorhabditis elegans] 3.0E-61
blast CAE65800 Hypothetical protein CBG10907 [Caenorhabditis briggsae] 5.0E-51
blast AAC48274 Hypothetical protein C53B7.3 [Caenorhabditis elegans] ref|NP_509153.1| C53B7.3 [Caenorhabditis elegans] 7.0E-49
blast CAE65800 Hypothetical protein CBG10907 [Caenorhabditis briggsae] 1.0E-46
blast AAC48274 Hypothetical protein C53B7.3 [Caenorhabditis elegans] ref|NP_509153.1| C53B7.3 [Caenorhabditis elegans] 2.0E-29
blast CAE65800 Hypothetical protein CBG10907 [Caenorhabditis briggsae] 5.0E-22
Sequence
Target Sequence 
>C. ELEGANS:180628_AT
tcgtctggccaaacttgctctaatggacaatgcatgagctctctcggatataacggagga
ctttactccaacacctataacaacaacaaccaatatgacatgaacagtcagtatggcaac
caaatgggtaccactggatccggaacatactgcaccacgtcgtactcttgccgatctgga
gaaacatgcatgaacaacagatgccaaagcagctattccaacaacatgtacaactccaat
tctcagtactctaactctggatccacttgccgttactctaccgactgctactctggacaa
atgtgctccaacggagtttgtgtcgcccaatacggaacctcttacaacaatgctatgtac
tcttcatctacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTCTGGCCAAACTTGCTCTAATG 452 605 127 Antisense
GGACAATGCATGAGCTCTCTCGGAT 194 527 151 Antisense
GAGGACTTTACTCCAACACCTATAA 310 403 182 Antisense
ACCAAATGGGTACCACTGGATCCGG 478 83 245 Antisense
CTGGATCCGGAACATACTGCACCAC 109 241 260 Antisense
GCCAAAGCAGCTATTCCAACAACAT 45 279 329 Antisense
TACAACTCCAATTCTCAGTACTCTA 182 643 355 Antisense
GTACTCTAACTCTGGATCCACTTGC 390 459 372 Antisense
ACCGACTGCTACTCTGGACAAATGT 223 89 406 Antisense
TCGCCCAATACGGAACCTCTTACAA 254 593 449 Antisense
AATGCTATGTACTCTTCATCTACAG 627 165 475 Antisense
Sequence Source Affymetrix Proprietary Database