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[180625_at]

Description T08B2.4 /REP_DB=WormBase Gene ID /WP=CE13425 /TR=Q9GYS5 /GB=AAG00014.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 29      4grp_high_8_8 > 03      6grp_highest_nonorm > 90      6grp_total > 29      4grp_total > 13      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 139.9
(-0.8)
P 		80
(-2.8)
A 		75
(-3)
P 		252.8
(3)
P 		178
B 158.9
(3)
P 		76.8
(-3)
P 		79
(-2.8)
P 		122.2
(0.3)
P 		82
C 129.2
(-0.8)
P 		73.5
(-3)
P 		91.8
(-2.3)
P 		222.3
(3)
P 		149
avr 142.7
(0.2) 		76.8
(-3) 		81.9
(-2.7) 		199.1
(3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 162.1
(2)
P 		170.4
(3)
P 		139.1
(-0.6)
P 		143.2
(-0.1)
P 		159.1
(1.7)
P 		118.5
(-3)
P 		52
B 138.3
(-0.5)
P 		140
(-0.1)
P 		129.5
(-3)
P 		131.5
(-2.4)
P 		148.6
(2.4)
P 		150.9
(3)
P 		21
C 235.2
(3)
P 		207.4
(1.1)
P 		152.2
(-2.6)
P 		146.6
(-3)
P 		183.3
(-0.5)
P 		179.5
(-0.8)
P 		89
avr 178.5
(3) 		172.6
(2.1) 		140.3
(-3) 		140.4
(-3) 		163.7
(0.7) 		149.6
(-1.5) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 51
(3)
P 		17.4
(-3)
A 		19.6
(-2.6)
P 		37
(0.5)
P 		34
B 50.7
(3)
P 		19
(-2.9)
P 		18.3
(-3)
P 		26
(-1.6)
P 		32
C 42.8
(3)
P 		21.1
(-3)
P 		32
(0)
P 		37.5
(1.5)
P 		22
avr 48.2
(3) 		19.2
(-3) 		23.3
(-2.2) 		33.5
(-0) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 29.9
(-2.8)
P 		60.6
(3)
P 		30.3
(-2.7)
P 		36.3
(-1.6)
P 		48
(0.6)
P 		28.8
(-3)
P 		32
B 48.1
(0.5)
P 		46.6
(0.1)
P 		31.4
(-3)
P 		34.8
(-2.3)
P 		60.4
(3)
P 		56.3
(2.2)
P 		29
C 87.2
(3)
P 		63.3
(0.5)
P 		38.7
(-2)
P 		29.5
(-3)
P 		59.3
(0.1)
P 		60.1
(0.2)
P 		58
avr 55.1
(2.6) 		56.8
(3) 		33.5
(-3) 		33.5
(-3) 		55.9
(2.8) 		48.4
(0.8) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180625_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2454
Sequence Type Exemplar sequence
Representative Public ID T08B2.4 NCBI
Target Description T08B2.4 /REP_DB=WormBase Gene ID /WP=CE13425 /TR=Q9GYS5 /GB=AAG00014.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171419(10), NM_059396(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171419 NCBI Caenorhabditis elegans R07H5.11 (R07H5.11) mRNA, complete cds. 10/11 None
NM_059396 NCBI Caenorhabditis elegans T08B2.4 (T08B2.4) mRNA, complete cds. 11/11 None
SNAP00000035007 ENSEMBL cdna:SNAP chromosome:CEL140:I:6233170:6234443:1 11/11 None
GENEFINDER00000035011 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6233170:6234099:1 11/11 None
T08B2.4 ENSEMBL cdna:known chromosome:CEL140:I:6233693:6234099:1 gene:T08B2.4 11/11 None
R07H5.11 ENSEMBL cdna:known chromosome:CEL140:IV:11202741:11203349:1 gene:R07H5.11 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030265 6/11 Cross Hyb Matching Probes None
SNAP00000015396 2/11 Cross Hyb Matching Probes None
GENEFINDER00000015408 2/11 Cross Hyb Matching Probes None
B0391.8 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11202946-11203353(+) 98.61 98.61
chrI:6233621-6234028(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl T08B2.4
R07H5.11
Entrez Gene 188273 Entrez gene
259596 Entrez gene
SwissProt Q8MYP7 EMBL-EBI
Q9GYS5 EMBL-EBI
WormBase CE30681 Wormbase
CE13425 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD31662 Hypothetical protein R07H5.11 [Caenorhabditis elegans] ref|NP_741491.1| R07H5.11 [Caenorhabditis elegans] 8.0E-65
blast NP_491797 T08B2.4 [Caenorhabditis elegans] gb|AAG00014.1| Hypothetical protein T08B2.4 [Caenorhabditis elegans] 3.0E-64
blast NP_491797 T08B2.4 [Caenorhabditis elegans] gb|AAG00014.1| Hypothetical protein T08B2.4 [Caenorhabditis elegans] 6.0E-64
blast NP_491797 T08B2.4 [Caenorhabditis elegans] gb|AAG00014.1| Hypothetical protein T08B2.4 [Caenorhabditis elegans] 7.0E-62
blast CAD31662 Hypothetical protein R07H5.11 [Caenorhabditis elegans] ref|NP_741491.1| R07H5.11 [Caenorhabditis elegans] 7.0E-62
blast CAD31662 Hypothetical protein R07H5.11 [Caenorhabditis elegans] ref|NP_741491.1| R07H5.11 [Caenorhabditis elegans] 1.0E-61
Sequence
Target Sequence 
>C. ELEGANS:180625_AT
ttcactatcttcaacaagtcgaactgactctcgtgttgacaaatgatccgtttgaggcgg
atcattatttggcacaaagagtggatctgtcagtatacgaatacattcaatcgtgtatgg
aacactcgaagaaagtattcacggatcgccgcatgcgttcagagtcatctcccaccaccc
atctccaaccacaccggatcatggtgctttcgaggaatcgctacgagtcaattggattac
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACTATCTTCAACAAGTCGAACT 180 687 20 Antisense
AGTCGAACTGACTCTCGTGTTGACA 276 61 36 Antisense
GACTCTCGTGTTGACAAATGATCCG 547 373 45 Antisense
GAGGCGGATCATTATTTGGCACAAA 68 407 73 Antisense
TTGGCACAAAGAGTGGATCTGTCAG 337 689 88 Antisense
GTGGATCTGTCAGTATACGAATACA 593 489 100 Antisense
GATCGCCGCATGCGTTCAGAGTCAT 463 417 163 Antisense
TCTCCAACCACACCGGATCATGGTG 509 609 201 Antisense
CCGGATCATGGTGCTTTCGAGGAAT 402 265 213 Antisense
GGTGCTTTCGAGGAATCGCTACGAG 536 499 222 Antisense
ATCGCTACGAGTCAATTGGATTACG 57 31 236 Antisense
Sequence Source Affymetrix Proprietary Database