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[180622_at]

Description F46H5.8 /REP_DB=WormBase Gene ID /WP=CE04594 /TR=Q20489 /GB=AAB37025.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 10      4grp_highest_8_8 > 51      4grp_high_8_8 > 25      6grp_total > 13      4grp_total > 47      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 181.4
(3)
P 		68.3
(-2.1)
P 		49.2
(-3)
P 		95.5
(-0.9)
P 		132
B 174.8
(3)
P 		80.4
(-1.9)
P 		58.3
(-3)
P 		92.9
(-1.2)
P 		117
C 207.3
(3)
P 		63.8
(-2.3)
P 		56.3
(-2.6)
P 		44.6
(-3)
P 		163
avr 187.8
(3) 		70.8
(-2.3) 		54.6
(-3) 		77.7
(-2) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 57.5
(-1.7)
P 		118.8
(3)
P 		75.1
(-0.4)
P 		94.4
(1.1)
P 		69.1
(-0.8)
P 		40.9
(-3)
A 		78
B 121.6
(3)
P 		82.8
(-0.1)
P 		99.5
(1.2)
P 		113.7
(2.4)
P 		91.6
(0.6)
P 		46.3
(-3)
P 		75
C 132.7
(3)
P 		107.7
(1)
P 		103.5
(0.7)
P 		117.1
(1.8)
P 		70.5
(-1.9)
P 		56.9
(-3)
P 		76
avr 103.9
(2.6) 		103.1
(2.5) 		92.7
(1.4) 		108.4
(3) 		77.1
(-0.1) 		48
(-3) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 66.1
(3)
P 		14.8
(-2.8)
P 		12.9
(-3)
P 		14
(-2.9)
P 		53
B 55.7
(3)
P 		19.9
(-2.1)
P 		13.5
(-3)
P 		19.8
(-2.1)
P 		42
C 68.6
(3)
P 		18.3
(-1.9)
P 		19.6
(-1.8)
P 		7.5
(-3)
P 		61
avr 63.5
(3) 		17.7
(-2.5) 		15.3
(-2.8) 		13.8
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 10.6
(-2.9)
P 		42.3
(3)
P 		16.4
(-1.8)
P 		23.9
(-0.4)
P 		20.9
(-1)
P 		10
(-3)
A 		32
B 42.3
(3)
P 		27.6
(-0.5)
P 		24.2
(-1.3)
P 		30.1
(0.1)
P 		37.2
(1.8)
P 		17.3
(-3)
P 		25
C 49.2
(3)
P 		32.9
(-0.2)
P 		26.3
(-1.6)
P 		23.5
(-2.1)
P 		22.8
(-2.3)
P 		19.1
(-3)
P 		30
avr 34
(2.9) 		34.3
(3) 		22.3
(-0.8) 		25.8
(0.3) 		27
(0.7) 		15.5
(-3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180622_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16994
Sequence Type Exemplar sequence
Representative Public ID F46H5.8 NCBI
Target Description F46H5.8 /REP_DB=WormBase Gene ID /WP=CE04594 /TR=Q20489 /GB=AAB37025.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076820(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076820 NCBI Caenorhabditis elegans F46H5.8 (F46H5.8) mRNA, complete cds. 11/11 None
GENEFINDER00000013763 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7258153:7260323:-1 11/11 None
F46H5.8 ENSEMBL cdna:known chromosome:CEL140:X:7258153:7260323:-1 gene:F46H5.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013756 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7258151-7260322(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46H5.8
Entrez Gene 185882 Entrez gene
SwissProt Q20489 EMBL-EBI
WormBase CE35542 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37025 Hypothetical protein F46H5.8 [Caenorhabditis elegans] ref|NP_509221.2| F46H5.8 [Caenorhabditis elegans] 0.0
blast CAE65863 Hypothetical protein CBG11006 [Caenorhabditis briggsae] 1.0E-132
blast AAB37025 Hypothetical protein F46H5.8 [Caenorhabditis elegans] ref|NP_509221.2| F46H5.8 [Caenorhabditis elegans] 1.0E-114
blast CAE65863 Hypothetical protein CBG11006 [Caenorhabditis briggsae] 3.0E-97
blast NP_001022015 C33F10.7a [Caenorhabditis elegans] gb|AAK31475.1| Hypothetical protein C33F10.7a [Caenorhabditis elegans] 5.0E-56
blast NP_001022015 C33F10.7a [Caenorhabditis elegans] gb|AAK31475.1| Hypothetical protein C33F10.7a [Caenorhabditis elegans] 8.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR001466 EMBL-EBI Beta-lactamase 1.4E-37
Pfam IPR001466 EMBL-EBI Beta-lactamase 2.7E-8
Pfam IPR001466 EMBL-EBI Beta-lactamase 3.8E-11
Sequence
Target Sequence 
>C. ELEGANS:180622_AT
aggactgcttaatccggaagctttgagaaaattcattgccggtgataatattcatcatca
aaagctgtattcaatatttccctggatcacgacggacgattataacttggtttctaatcg
tcttctgccaatgccatccaacatgggaatatcgaatgctgagggactcgcgcattttca
ttcgttggttgctaacaagcaaatcctctcggaaaacatgtatagattattaaagcaacc
ggtgttagaacatgtatatgatcatgcaattggatatgaagaaaacaaaggatacggatt
tcagtatactaaaaatccaaagggacaatggatatttgggcactctggatttggcggaca
aaatgtcagagtcgatgtaactaatggactcacactggcctatgtatcgaatggaatgaa
aatcactgacgcggatatggtggagccttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGACTGCTTAATCCGGAAGCTTTG 373 53 357 Antisense
GCTGTATTCAATATTTCCCTGGATC 699 295 420 Antisense
ATTTCCCTGGATCACGACGGACGAT 313 17 432 Antisense
AACTTGGTTTCTAATCGTCTTCTGC 145 137 460 Antisense
TTCTGCCAATGCCATCCAACATGGG 47 691 479 Antisense
TATCGAATGCTGAGGGACTCGCGCA 64 657 506 Antisense
TCGCGCATTTTCATTCGTTGGTTGC 128 599 524 Antisense
GCTAACAAGCAAATCCTCTCGGAAA 707 301 547 Antisense
GGATATTTGGGCACTCTGGATTTGG 74 509 686 Antisense
GACTCACACTGGCCTATGTATCGAA 685 373 743 Antisense
GACGCGGATATGGTGGAGCCTTGGA 71 381 784 Antisense
Sequence Source Affymetrix Proprietary Database