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[180618_at]

Description F14D2.7 /REP_DB=WormBase Gene ID /WP=CE16982 /TR=O44801 /GB=AAB94961.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 30      6grp_total > 41      4grp_total > 54      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 197.8
(3)
P 		107.2
(0.1)
A 		38.4
(-2.1)
A 		8.7
(-3)
A 		189
B 183.9
(3)
P 		94.5
(-0.5)
P 		57.6
(-1.9)
P 		30.2
(-3)
A 		154
C 251.7
(3)
P 		55.2
(-1.9)
P 		63.9
(-1.7)
P 		10.4
(-3)
A 		241
avr 211.1
(3) 		85.6
(-0.9) 		53.3
(-1.9) 		16.4
(-3) 		195

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 200.5
(0.5)
P 		144
(-0.6)
P 		322.8
(3)
P 		109.8
(-1.3)
P 		73.6
(-2)
P 		25.5
(-3)
A 		297
B 256.1
(0.8)
P 		212.9
(-0)
P 		370.7
(3)
P 		200.1
(-0.3)
P 		60.2
(-3)
P 		71.5
(-2.8)
P 		311
C 96.9
(-2.4)
P 		280.6
(3)
P 		124
(-1.6)
P 		241.8
(1.8)
P 		78.2
(-3)
P 		100
(-2.4)
P 		202
avr 184.5
(0.4) 		212.5
(1.3) 		272.5
(3) 		183.9
(0.4) 		70.7
(-2.9) 		65.7
(-3) 		207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 72
(3)
P 		23.3
(-1.1)
A 		10
(-2.3)
A 		1.3
(-3)
A 		71
B 58.7
(3)
P 		23.3
(-1.1)
P 		13.3
(-2.2)
P 		6.4
(-3)
A 		52
C 83.3
(3)
P 		15.9
(-2)
P 		22.3
(-1.5)
P 		1.8
(-3)
A 		82
avr 71.3
(3) 		20.8
(-1.4) 		15.2
(-1.9) 		3.2
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 37
(-0.1)
P 		51.2
(1.2)
P 		70.3
(3)
P 		27.8
(-1)
P 		22.2
(-1.5)
P 		6.2
(-3)
A 		64
B 89.1
(2.9)
P 		70.9
(1.3)
P 		90
(3)
P 		53
(-0.4)
P 		24.5
(-3)
P 		26.7
(-2.8)
P 		66
C 35.9
(-1.9)
P 		85.6
(3)
P 		31.6
(-2.4)
P 		48.6
(-0.7)
P 		25.3
(-3)
P 		33.5
(-2.2)
P 		60
avr 54
(1.1) 		69.2
(3) 		64
(2.3) 		43.1
(-0.3) 		24
(-2.8) 		22.1
(-3) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180618_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4170
Sequence Type Exemplar sequence
Representative Public ID F14D2.7 NCBI
Target Description F14D2.7 /REP_DB=WormBase Gene ID /WP=CE16982 /TR=O44801 /GB=AAB94961.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062108(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062108 NCBI Caenorhabditis elegans F14D2.7 (F14D2.7) mRNA, complete cds. 11/11 None
SNAP00000037057 ENSEMBL cdna:SNAP chromosome:CEL140:II:3335686:3336788:-1 9/11 None
GENEFINDER00000037082 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3335321:3337111:-1 10/11 None
F14D2.7 ENSEMBL cdna:known chromosome:CEL140:II:3335686:3338651:-1 gene:F14D2.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3335684-3338650(-) 98.45 100.0
Public Domain and Genome References
Gene Title
Ensembl F14D2.7
Entrez Gene 184458 Entrez gene
SwissProt O44801 EMBL-EBI
WormBase CE16982 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494509 F14D2.7 [Caenorhabditis elegans] gb|AAB94961.1| Hypothetical protein F14D2.7 [Caenorhabditis elegans] 0.0
blast NP_494509 F14D2.7 [Caenorhabditis elegans] gb|AAB94961.1| Hypothetical protein F14D2.7 [Caenorhabditis elegans] 1.0E-175
blast NP_494509 F14D2.7 [Caenorhabditis elegans] gb|AAB94961.1| Hypothetical protein F14D2.7 [Caenorhabditis elegans] 1.0E-125
blast AAK68324 Hypothetical protein F14H12.2 [Caenorhabditis elegans] ref|NP_508746.1| F14H12.2 [Caenorhabditis elegans] 1.0E-30
blast CAA16274 Hypothetical protein F19B2.7 [Caenorhabditis elegans] ref|NP_507884.1| F19B2.7 [Caenorhabditis elegans] 1.0E-16
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494509.1 1 13-35
F14D2.7 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:180618_AT
ggaagtaacattctgcaatagttgcaacgagattcgtaacagatgtgaagaagatgagtt
gacaagatgtccattttgtggaaacgctgagttgacggaaggaaagctaatgacacatat
tgactcatgttttgtgatcacaggggaccaatttacaattttgaggcctaatgaacattt
taaaatcaattcagtcaactataggctgaatgcctttgcacagtacattgaatcaatgaa
caacgaagaggactgtgggcattacgtttcatggactcgagaattaaaaaaggaaggaaa
tttcgaagaaacaaagaacccgaatacaactacgaaaaataacaatgtagaggctattac
agaaattcaaaaggatcaagctgaacacgcaaaggacactaataataataataataataa
tgaacatttacacgatgaatataatataattcaaacagaagataacaatgaagaagaaaa
acactataccgaatattcgattgatcaagatttcgaatgcattgatgacgataaacatta
cagtgggaagcattcaccagttgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGTAACATTCTGCAATAGTTGC 673 531 825 Antisense
GTTGACAAGATGTCCATTTTGTGGA 667 437 882 Antisense
GTGGAAACGCTGAGTTGACGGAAGG 230 491 902 Antisense
ATTGACTCATGTTTTGTGATCACAG 327 5 943 Antisense
GTGATCACAGGGGACCAATTTACAA 368 483 958 Antisense
GGCTGAATGCCTTTGCACAGTACAT 60 539 1028 Antisense
GAGGACTGTGGGCATTACGTTTCAT 597 403 1072 Antisense
GGGCATTACGTTTCATGGACTCGAG 552 489 1081 Antisense
CAAGCTGAACACGCAAAGGACACTA 293 185 1201 Antisense
AAAAACACTATACCGAATATTCGAT 684 127 1301 Antisense
CAGTGGGAAGCATTCACCAGTTGAG 137 207 1365 Antisense
Sequence Source Affymetrix Proprietary Database