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[180615_at]

Description F49C12.6 /REP_DB=WormBase Gene ID /WP=CE03366 /TR=Q20583 /GB=CAA92510.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 62      6grp_total > 31      4grp_total > 03      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1615
1 2 3 4 range
A 848.9
(-2.1)
P 		879.3
(-2)
P 		653.3
(-3)
P 		1982.3
(3)
P 		1329
B 1070.6
(-1.2)
P 		638.3
(-3)
P 		685.3
(-2.8)
P 		2058.6
(3)
P 		1420
C 810.7
(-2)
P 		641.2
(-2.6)
P 		546
(-3)
P 		2160.8
(3)
P 		1615
avr 910.1
(-1.8) 		719.6
(-2.6) 		628.2
(-3) 		2067.2
(3) 		1439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 527.3
(-0.1)
P 		616.2
(1.1)
P 		763.8
(3)
P 		723.6
(2.5)
P 		432.1
(-1.4)
P 		307.9
(-3)
P 		456
B 444.4
(-1.6)
P 		608.6
(1.1)
P 		723.9
(3)
P 		481.7
(-1)
P 		421.9
(-2)
P 		362.9
(-3)
P 		361
C 480.5
(-0.4)
P 		618.4
(2.1)
P 		666.7
(3)
P 		562.5
(1.1)
P 		349.2
(-2.7)
P 		335
(-3)
P 		332
avr 484.1
(-0.7) 		614.4
(1.4) 		718.1
(3) 		589.3
(1) 		401.1
(-2) 		335.3
(-3) 		383
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 309.1
(3)
P 		190.9
(-2.1)
P 		170.8
(-3)
P 		290.2
(2.2)
P 		138
B 341.4
(0.9)
P 		157.6
(-3)
P 		158.8
(-3)
P 		438.7
(3)
P 		281
C 268.4
(-0.2)
P 		184.3
(-3)
P 		190.3
(-2.8)
P 		364.3
(3)
P 		180
avr 306.3
(1.2) 		177.6
(-2.9) 		173.3
(-3) 		364.4
(3) 		191

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 97.2
(-2.1)
P 		219.2
(3)
P 		166.3
(0.8)
P 		183.5
(1.5)
P 		130.4
(-0.7)
P 		75
(-3)
P 		144
B 154.6
(-0.8)
P 		202.6
(3)
P 		175.8
(0.9)
P 		127.6
(-3)
P 		171.4
(0.5)
P 		135.3
(-2.4)
P 		75
C 178.1
(2.2)
P 		188.7
(3)
P 		169.7
(1.5)
P 		113.1
(-2.9)
P 		113
(-2.9)
P 		112.2
(-3)
P 		77
avr 143.3
(-0.8) 		203.5
(3) 		170.6
(0.9) 		141.4
(-0.9) 		138.3
(-1.1) 		107.5
(-3) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180615_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9678
Sequence Type Exemplar sequence
Representative Public ID F49C12.6 NCBI
Target Description F49C12.6 /REP_DB=WormBase Gene ID /WP=CE03366 /TR=Q20583 /GB=CAA92510.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069228(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069228 NCBI Caenorhabditis elegans F49C12.6 (F49C12.6) mRNA, complete cds. 11/11 None
SNAP00000032694 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9307363:9309042:-1 11/11 None
GENEFINDER00000032707 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9307363:9309042:-1 11/11 None
F49C12.6 ENSEMBL cdna:known chromosome:CEL140:IV:9307363:9309042:-1 gene:F49C12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9307368-9309048(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49C12.6
Entrez Gene 186030 Entrez gene
SwissProt Q20583 EMBL-EBI
WormBase CE03366 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92510 Hypothetical protein F49C12.6 [Caenorhabditis elegans] ref|NP_501629.1| F49C12.6 [Caenorhabditis elegans] 0.0
blast CAE73596 Hypothetical protein CBG21080 [Caenorhabditis briggsae] 1.0E-170
blast CAE73580 Hypothetical protein CBG21054 [Caenorhabditis briggsae] 1.0E-110
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501629.1 9 7-29,44-66,86-108,123-142,177-199,214-236,243-265,275-297,306-325
F49C12.6 9 7-29,44-66,86-108,123-142,177-199,214-236,243-265,275-297,306-325
SNAP00000032694 9 7-29,44-66,86-108,123-142,177-199,214-236,243-265,275-297,306-325
GENEFINDER00000032707 9 7-29,44-66,86-108,123-142,177-199,214-236,243-265,275-297,306-325
Sequence
Target Sequence 
>C. ELEGANS:180615_AT
gaggagctatttatgcaccgatcaagcatatccccgcaagagtgagacctgctccatgga
gtttggaagaagataattataaagatgacccattggttgtgaaggaggtttcgtgcacaa
gaagaatattatgcctattcctaacaattttcgttggtttcctttacggcaacttcttga
ccccaatcaactatataattgccaatgagcccggttcacatcaagatgttcgtgcataca
tcctttcttattgtcttggatcatttgttacatctactgtaatcttcttcgtttactcaa
ttgtaaagaagaacgttccacttgtgaatgcggagttatcaatgccatctattatttctg
gaattttatatggaattgcggtcacaacatttttcatggcaaatcaacacttggatcaag
ttatcgcgtacccaattctttccaaagctcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGAGCTATTTATGCACCGATCAA 138 405 416 Antisense
AGCATATCCCCGCAAGAGTGAGACC 112 79 440 Antisense
AGAGTGAGACCTGCTCCATGGAGTT 298 67 454 Antisense
CAACTTCTTGACCCCAATCAACTAT 137 189 585 Antisense
ATAATTGCCAATGAGCCCGGTTCAC 488 25 610 Antisense
GTGCATACATCCTTTCTTATTGTCT 154 481 647 Antisense
TAATCTTCTTCGTTTACTCAATTGT 671 635 695 Antisense
GAACGTTCCACTTGTGAATGCGGAG 184 347 726 Antisense
GCGGTCACAACATTTTTCATGGCAA 269 293 793 Antisense
GGATCAAGTTATCGCGTACCCAATT 185 511 828 Antisense
TACCCAATTCTTTCCAAAGCTCCTG 430 647 844 Antisense
Sequence Source Affymetrix Proprietary Database