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[180609_at]

Description T05A10.2 /REP_DB=WormBase Gene ID /WP=CE03634 /TR=Q22191 /GB=CAA92134.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 0      4grp_total > 19      6grp_total_sub > Hat      6grp_cgc > 64      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 26.3
(-2)
A 		90
(3)
A 		13.1
(-3)
A 		80.2
(2.2)
M 		77
B 41.4
(0.1)
A 		34.7
(-0.5)
A 		6.5
(-3)
A 		73.5
(3)
A 		67
C 49.8
(-1.1)
A 		28.1
(-3)
A 		45.2
(-1.5)
A 		97.7
(3)
A 		70
avr 39.2
(-1.3) 		50.9
(-0.2) 		21.6
(-3) 		83.8
(3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 22
(-3)
A 		43.9
(2.6)
A 		29.9
(-1)
M 		22.4
(-2.9)
A 		45.6
(3)
A 		44.1
(2.6)
A 		24
B 30
(-0.8)
A 		49
(3)
P 		43.8
(2)
A 		32.8
(-0.2)
A 		18.9
(-3)
A 		19.6
(-2.9)
A 		30
C 49.7
(0.4)
A 		44.2
(-0.3)
A 		23.4
(-3)
A 		69.5
(3)
A 		48.4
(0.3)
A 		57.1
(1.4)
A 		46
avr 33.9
(-2.3) 		45.7
(3) 		32.4
(-3) 		41.6
(1.2) 		37.6
(-0.7) 		40.3
(0.6) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 9.6
(-0.7)
A 		19.5
(3)
A 		3.4
(-3)
A 		11.7
(0.1)
M 		16
B 13.2
(1.9)
A 		8.6
(0)
A 		1.5
(-3)
A 		15.7
(3)
A 		14
C 16.5
(3)
A 		8.1
(-3)
A 		15.7
(2.4)
A 		16.5
(3)
A 		8
avr 13.1
(1.8) 		12.1
(1.1) 		6.9
(-3) 		14.6
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 4
(-3)
A 		15.6
(3)
A 		6.5
(-1.7)
M 		5.7
(-2.1)
A 		13.8
(2.1)
A 		10.7
(0.5)
A 		12
B 10.4
(-0.9)
A 		16.3
(3)
P 		10.6
(-0.8)
A 		8.7
(-2.1)
A 		7.7
(-2.7)
A 		7.3
(-3)
A 		9
C 18.4
(2.7)
A 		13.5
(0.5)
A 		5.9
(-3)
A 		14
(0.7)
A 		15.6
(1.4)
A 		19.1
(3)
A 		13
avr 10.9
(-0.4) 		15.1
(3) 		7.7
(-3) 		9.5
(-1.5) 		12.4
(0.8) 		12.4
(0.8) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180609_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16100
Sequence Type Exemplar sequence
Representative Public ID T05A10.2 NCBI
Target Description T05A10.2 /REP_DB=WormBase Gene ID /WP=CE03634 /TR=Q22191 /GB=CAA92134.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077399(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077399 NCBI Caenorhabditis elegans CLaudin-like in Caenorhabditis family member (clc-4) (clc-4) mRNA, complete cds. 11/11 None
SNAP00000000836 ENSEMBL cdna:SNAP chromosome:CEL140:X:10782919:10783830:-1 9/11 None
GENEFINDER00000000840 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10782919:10783830:-1 11/11 None
T05A10.2 ENSEMBL cdna:known chromosome:CEL140:X:10782919:10783830:-1 gene:T05A10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10782918-10783830(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol clc-4
Ensembl T05A10.2
Entrez Gene 188095 Entrez gene
SwissProt Q22191 EMBL-EBI
WormBase CE03634 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92134 Hypothetical protein T05A10.2 [Caenorhabditis elegans] ref|NP_509800.1| CLaudin-like in Caenorhabditis family member (clc-4) [Caenorhabditis elegans] 1.0E-110
blast CAE70823 Hypothetical protein CBG17594 [Caenorhabditis briggsae] 1.0E-88
blast CAA92134 Hypothetical protein T05A10.2 [Caenorhabditis elegans] ref|NP_509800.1| CLaudin-like in Caenorhabditis family member (clc-4) [Caenorhabditis elegans] 1.0E-81
blast CAE70823 Hypothetical protein CBG17594 [Caenorhabditis briggsae] 5.0E-61
blast CAB01719 Hypothetical protein C09F12.1 [Caenorhabditis elegans] ref|NP_509847.1| CLaudin-like in Caenorhabditis family member (clc-1) [Caenorhabditis elegans] 1.0E-27
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509800.1 4 5-27,80-102,117-136,166-188
T05A10.2 4 5-27,80-102,117-136,166-188
SNAP00000000836 3 5-27,75-97,130-152
GENEFINDER00000000840 4 5-27,80-102,117-136,166-188
Sequence
Target Sequence 
>C. ELEGANS:180609_AT
tgtttggggttgtcatgctgggagctattgctctcacccttggagccactttttccgaca
agtggcgcactgtggaaaaggaccttctggatctttatgaagacgttcaacgcaaccata
cagaacgtttgacgggaatacttccattcttgtgtgaagatggtgctgttggatgtgcgg
gattttggaaggaaatgaaaccctatgaaaaaattgtcgcaatttgtatgatcgcagcgt
taattttggaaattgtcgctttcgtttggaatttcctaacctcatgcacctgttgcttca
agaaatatttacttcatccactatccccactctcattcctcatcacaatttttctgactg
ttgccattggagttttctattataacagcgatgaaattaaagatgatgtcaatatccaga
agatttgggatgcggaagcaaaggatttgaatattaatattggatccgctttctggatgg
ccgtaggagcatggtgtcttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTTGGGGTTGTCATGCTGGGAGC 685 559 38 Antisense
ATGCTGGGAGCTATTGCTCTCACCC 155 43 52 Antisense
CTTGGAGCCACTTTTTCCGACAAGT 340 215 76 Antisense
GACGGGAATACTTCCATTCTTGTGT 623 375 168 Antisense
TTGTCGCTTTCGTTTGGAATTTCCT 394 667 290 Antisense
CCTCATGCACCTGTTGCTTCAAGAA 41 269 317 Antisense
AAATATTTACTTCATCCACTATCCC 541 115 340 Antisense
CTCATTCCTCATCACAATTTTTCTG 12 231 369 Antisense
ATTTTTCTGACTGTTGCCATTGGAG 459 15 385 Antisense
TATTGGATCCGCTTTCTGGATGGCC 536 665 495 Antisense
TGGCCGTAGGAGCATGGTGTCTTGT 483 543 515 Antisense
Sequence Source Affymetrix Proprietary Database