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[180606_at]

Description F40G12.5 /REP_DB=WormBase Gene ID /WP=CE10184 /TR=Q20246 /GB=CAB01183.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 96      6grp_total > 87      4grp_total > 02      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 991
1 2 3 4 range
A 39.1
(-3)
A 		39.2
(-3)
A 		33.6
(-3)
A 		999.5
(3)
P 		966
B 46.3
(-2.8)
A 		28
(-2.9)
A 		9.5
(-3)
A 		951.1
(3)
P 		942
C 36.1
(-2.8)
M 		20.1
(-2.9)
A 		8.5
(-3)
A 		912.3
(3)
P 		904
avr 40.5
(-2.9) 		29.1
(-2.9) 		17.2
(-3) 		954.3
(3) 		937

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1019
1 2 3 4 5 6 range
A 90.4
(-2.7)
P 		56.8
(-3)
A 		163
(-2.2)
P 		368.8
(-0.7)
P 		503.3
(0.4)
P 		856.3
(3)
P 		800
B 87.4
(-3)
P 		85.8
(-3)
P 		167
(-2.5)
P 		272.5
(-1.9)
P 		492.6
(-0.5)
P 		1064.3
(3)
P 		979
C 64.3
(-2.9)
P 		45.1
(-3)
P 		115.2
(-2.5)
P 		334.6
(-1)
P 		434.6
(-0.3)
P 		917.1
(3)
P 		872
avr 80.7
(-2.9) 		62.6
(-3) 		148.4
(-2.4) 		325.3
(-1.2) 		476.8
(-0.2) 		945.9
(3) 		883
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 14.2
(-2.8)
A 		8.5
(-3)
A 		8.8
(-3)
A 		146.3
(3)
P 		138
B 14.8
(-2.6)
A 		6.9
(-2.9)
A 		2.2
(-3)
A 		202.7
(3)
P 		201
C 11.9
(-2.6)
M 		5.8
(-2.9)
A 		3
(-3)
A 		153.8
(3)
P 		151
avr 13.6
(-2.7) 		7.1
(-2.9) 		4.7
(-3) 		167.6
(3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 383
1 2 3 4 5 6 range
A 16.7
(-3)
P 		20.2
(-2.9)
A 		35.5
(-2.4)
P 		93.5
(-0.6)
P 		151.9
(1.2)
P 		208.5
(3)
P 		192
B 30.4
(-3)
P 		28.6
(-3)
P 		40.6
(-2.8)
P 		72.2
(-2.3)
P 		200.1
(-0.2)
P 		396.9
(3)
P 		368
C 23.8
(-2.8)
P 		13.8
(-3)
P 		29.3
(-2.7)
P 		67.3
(-1.9)
P 		140.6
(-0.4)
P 		307
(3)
P 		293
avr 23.6
(-2.9) 		20.9
(-3) 		35.1
(-2.7) 		77.7
(-1.8) 		164.2
(0) 		304.1
(3) 		283
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180606_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12630
Sequence Type Exemplar sequence
Representative Public ID F40G12.5 NCBI
Target Description F40G12.5 /REP_DB=WormBase Gene ID /WP=CE10184 /TR=Q20246 /GB=CAB01183.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074131(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074131 NCBI Caenorhabditis elegans F40G12.5 (F40G12.5) mRNA, complete cds. 11/11 None
SNAP00000026729 ENSEMBL cdna:SNAP chromosome:CEL140:V:14270349:14273224:-1 11/11 None
GENEFINDER00000026759 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14271858:14273224:-1 11/11 None
F40G12.5 ENSEMBL cdna:known chromosome:CEL140:V:14271858:14273224:-1 gene:F40G12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14271857-14273224(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40G12.5
Entrez Gene 185565 Entrez gene
SwissProt Q20246 EMBL-EBI
WormBase CE10184 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01183 Hypothetical protein F40G12.5 [Caenorhabditis elegans] ref|NP_506532.1| F40G12.5 [Caenorhabditis elegans] 1.0E-154
blast CAB01183 Hypothetical protein F40G12.5 [Caenorhabditis elegans] ref|NP_506532.1| F40G12.5 [Caenorhabditis elegans] 1.0E-142
blast CAB01187 Hypothetical protein F40G12.4 [Caenorhabditis elegans] ref|NP_506531.2| F40G12.4 [Caenorhabditis elegans] 1.0E-119
blast CAE60977 Hypothetical protein CBG04711 [Caenorhabditis briggsae] 1.0E-108
blast CAE60977 Hypothetical protein CBG04711 [Caenorhabditis briggsae] 1.0E-100
blast CAB01187 Hypothetical protein F40G12.4 [Caenorhabditis elegans] ref|NP_506531.2| F40G12.4 [Caenorhabditis elegans] 5.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 3.6E-52
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 1.6E-5
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 6.1E-6
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 6.3E-36
Sequence
Target Sequence 
>C. ELEGANS:180606_AT
agacgagaagccttaacctctgggaaggaatgtttcctggaaatagttaaggaagaatgc
ccggaagaaaagtttaaactcatcgaagaaaactatagtcaacttgtgacacttctgact
gaaaaaccaaaagataatggagcatgtactgctccatattttcaattagaagaaattgag
tgcaacgctcataagcatgctcttcaattagaaatgcaggaacaaactggagagaaggag
actcatgatggagctgttaaggtgctgaaaatgtgcaaaaatgctgagacttgcgtgcat
gactcttgcaagttcacaaatttcgagcgagaagagattgagaacagctgtgatgtgctc
gaactgacaacatctgacttcacagtgtgcatgaacaagatcaacaaggagaagcctgat
ttatcaaaatacgaatgcctcaaggatcatgatttctatagcaaagattccgcggcaatt
tgtgatcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACGAGAAGCCTTAACCTCTGGGA 300 71 199 Antisense
GTCAACTTGTGACACTTCTGACTGA 159 465 296 Antisense
GCATGTACTGCTCCATATTTTCAAT 227 311 340 Antisense
GCAACGCTCATAAGCATGCTCTTCA 70 321 380 Antisense
GCTGAGACTTGCGTGCATGACTCTT 562 295 481 Antisense
GCATGACTCTTGCAAGTTCACAAAT 53 311 495 Antisense
GAACAGCTGTGATGTGCTCGAACTG 568 351 540 Antisense
GTGCTCGAACTGACAACATCTGACT 690 479 553 Antisense
AACATCTGACTTCACAGTGTGCATG 83 139 567 Antisense
AATGCCTCAAGGATCATGATTTCTA 164 167 632 Antisense
AAGATTCCGCGGCAATTTGTGATCG 514 155 662 Antisense
Sequence Source Affymetrix Proprietary Database