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[180594_at]

Description F54C1.8 /REP_DB=WormBase Gene ID /WP=CE11054 /TR=P91325 /GB=AAK21396.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 20      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 27.1
(-1.8)
A 		32.9
(-1.5)
A 		3.9
(-3)
A 		119.7
(3)
A 		116
B 38.9
(1)
A 		57.1
(3)
A 		3.2
(-3)
A 		33.3
(0.4)
A 		54
C 36.5
(-1.5)
A 		8.8
(-2.9)
A 		6.5
(-3)
A 		125.4
(3)
A 		119
avr 34.2
(-1) 		32.9
(-1.1) 		4.5
(-3) 		92.8
(3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 79.9
(-0.7)
A 		27.3
(-2.3)
A 		5.4
(-3)
A 		48.9
(-1.6)
A 		156.3
(1.7)
A 		198.1
(3)
P 		193
B 15.2
(-3)
A 		19
(-2.9)
A 		47.4
(-1.9)
A 		44.3
(-2)
A 		183.5
(3)
P 		159.3
(2.1)
P 		168
C 14.3
(-2.7)
A 		80.5
(-0.5)
A 		5.2
(-3)
A 		77
(-0.6)
A 		91.6
(-0.2)
A 		187.7
(3)
P 		183
avr 36.5
(-2.4) 		42.3
(-2.2) 		19.3
(-3) 		56.7
(-1.6) 		143.8
(1.6) 		181.7
(3) 		162
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 9.9
(0.2)
A 		7.1
(-0.8)
A 		1
(-3)
A 		17.5
(3)
A 		17
B 12.4
(2.2)
A 		14.1
(3)
A 		0.7
(-3)
A 		7.1
(-0.1)
A 		13
C 12.1
(0.1)
A 		2.5
(-2.9)
A 		2.3
(-3)
A 		21.1
(3)
A 		19
avr 11.5
(1.4) 		7.9
(-0.2) 		1.3
(-3) 		15.2
(3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 14.7
(-1.3)
A 		9.7
(-1.9)
A 		1.2
(-3)
A 		12.4
(-1.6)
A 		47.2
(2.9)
A 		48.2
(3)
P 		47
B 5.3
(-3)
A 		6.3
(-2.9)
A 		11.5
(-2.5)
A 		11.7
(-2.4)
A 		74.5
(3)
P 		59.4
(1.7)
P 		69
C 5.3
(-2.6)
A 		24.6
(-0.7)
A 		1.3
(-3)
A 		15.5
(-1.6)
A 		29.6
(-0.2)
A 		62.8
(3)
P 		62
avr 8.4
(-2.6) 		13.5
(-2) 		4.7
(-3) 		13.2
(-2) 		50.4
(2.3) 		56.8
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180594_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.447
Sequence Type Exemplar sequence
Representative Public ID F54C1.8 NCBI
Target Description F54C1.8 /REP_DB=WormBase Gene ID /WP=CE11054 /TR=P91325 /GB=AAK21396.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059096(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059096 NCBI Caenorhabditis elegans F54C1.8 (F54C1.8) mRNA, complete cds. 10/11 None
SNAP00000015271 ENSEMBL cdna:SNAP chromosome:CEL140:I:5004960:5006012:-1 10/11 None
GENEFINDER00000015281 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5004960:5006012:-1 11/11 None
F54C1.8 ENSEMBL cdna:known chromosome:CEL140:I:5004960:5006012:-1 gene:F54C1.8 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182462 4/11 Cross Hyb Matching Probes None
Y108F1.2 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:265403-269051(+) 98.26 98.84
chrI:5004959-5006012(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54C1.8
Entrez Gene 186220 Entrez gene
SwissProt P91325 EMBL-EBI
WormBase CE34315 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21396 Hypothetical protein F54C1.8 [Caenorhabditis elegans] ref|NP_491497.2| F54C1.8 [Caenorhabditis elegans] 1.0E-109
blast AAK21396 Hypothetical protein F54C1.8 [Caenorhabditis elegans] ref|NP_491497.2| F54C1.8 [Caenorhabditis elegans] 1.0E-106
blast NP_872262 Y108F1.2 [Caenorhabditis elegans] 5.0E-35
blast NP_872262 Y108F1.2 [Caenorhabditis elegans] 5.0E-32
blast CAE58964 Hypothetical protein CBG02235 [Caenorhabditis briggsae] 1.0E-31
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491497.2 4 48-70,80-102,109-131,151-173
F54C1.8 4 48-70,80-102,109-131,151-173
SNAP00000015271 4 48-70,80-102,109-131,151-173
GENEFINDER00000015281 4 46-68,101-120,127-149,169-191
Sequence
Target Sequence 
>C. ELEGANS:180594_AT
gagggatcgattcggactgtggcctgaaggcctgtattgcgtcttttgcattctcttcgg
aattgctcaattttatgctgccacttctaatttcgaaacccgggaagtcgcaatcataac
gagaagatgtttcgcagctgttgatgtcagctccgccctgattccaaaaactaaaatcca
attttttcagatctttgctggtttgctcgcatttgttctaattttcaaatcggtccgaat
catctacattgtactgacggttttctcaggaataaccattgggctcgagtcgttgacttt
catggacacaatctatttgcttcaacgtttgccttcacttggcatcgataagaaatatga
gaattgtgtattttatctgttcattgtcgctcttgtagtgactctttggaaaattgttct
cggcatcttttgtttctatgtgtttttgatgagatcttgtaaaagtcaattaactctgga
gactgaaagaagaaagaaaagagatcgctcgtgtacacctgagaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGGATCGATTCGGACTGTGGCCT 587 401 138 Antisense
GTATTGCGTCTTTTGCATTCTCTTC 289 453 171 Antisense
TTTATGCTGCCACTTCTAATTTCGA 186 673 209 Antisense
AGATCTTTGCTGGTTTGCTCGCATT 653 65 326 Antisense
AATCGGTCCGAATCATCTACATTGT 188 169 365 Antisense
TAACCATTGGGCTCGAGTCGTTGAC 65 629 410 Antisense
GCTTCAACGTTTGCCTTCACTTGGC 569 305 456 Antisense
TTTTATCTGTTCATTGTCGCTCTTG 54 677 508 Antisense
GTCGCTCTTGTAGTGACTCTTTGGA 298 475 523 Antisense
AAATTGTTCTCGGCATCTTTTGTTT 484 115 548 Antisense
AGATCGCTCGTGTACACCTGAGAAA 368 63 639 Antisense
Sequence Source Affymetrix Proprietary Database