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[180579_at]

Description F48E8.4 /REP_DB=WormBase Gene ID /WP=CE02757 /TR=Q09396 /GB=AAC46544.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 64      4grp_high_8_8 > 05      6grp_total > 43      4grp_total > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 93.4
(0.5)
P 		44
(-3)
A 		69
(-1.2)
P 		128.5
(3)
P 		85
B 70.2
(-2.5)
P 		74.8
(-2)
P 		64.6
(-3)
P 		128.2
(3)
P 		64
C 74.7
(-2.7)
P 		69.7
(-3)
P 		69.9
(-3)
P 		171.9
(3)
P 		102
avr 79.4
(-1.8) 		62.8
(-3) 		67.8
(-2.6) 		142.9
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 113.1
(2.3)
P 		45.8
(-2.3)
P 		123
(3)
P 		84.6
(0.4)
P 		42.5
(-2.5)
M 		35
(-3)
A 		88
B 59.2
(-2)
P 		74.7
(-0.2)
P 		103.5
(3)
P 		50.1
(-3)
P 		57.3
(-2.2)
P 		83.7
(0.8)
P 		53
C 64.4
(-0.8)
P 		91.1
(2.7)
P 		59.5
(-1.4)
P 		85.4
(2)
P 		47.2
(-3)
P 		93.1
(3)
P 		46
avr 78.9
(0.9) 		70.5
(-0.2) 		95.3
(3) 		73.4
(0.2) 		49
(-3) 		70.6
(-0.2) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 34
(3)
P 		9.6
(-3)
A 		18
(-0.9)
P 		18.8
(-0.7)
P 		24
B 22.4
(0.6)
P 		18.5
(-1.3)
P 		15
(-3)
P 		27.3
(3)
P 		12
C 24.7
(0.1)
P 		20
(-3)
P 		24.4
(-0.1)
P 		29
(3)
P 		9
avr 27
(3) 		16
(-3) 		19.1
(-1.3) 		25
(1.9) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 20.9
(1.1)
P 		16.3
(-0.4)
P 		26.8
(3)
P 		21.5
(1.3)
P 		12.8
(-1.6)
M 		8.5
(-3)
A 		18
B 20.6
(-0.6)
P 		24.9
(0.9)
P 		25.1
(1)
P 		13.3
(-3)
P 		23.3
(0.4)
P 		31.2
(3)
P 		18
C 23.9
(0.3)
P 		27.8
(1.7)
P 		15.1
(-3)
P 		17.2
(-2.2)
P 		15.3
(-2.9)
P 		31.2
(3)
P 		16
avr 21.8
(1.3) 		23
(2.4) 		22.3
(1.8) 		17.3
(-2.8) 		17.1
(-3) 		23.6
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180579_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7482
Sequence Type Exemplar sequence
Representative Public ID F48E8.4 NCBI
Target Description F48E8.4 /REP_DB=WormBase Gene ID /WP=CE02757 /TR=Q09396 /GB=AAC46544.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065762(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065762 NCBI Caenorhabditis elegans F48E8.4 (F48E8.4) mRNA, complete cds. 11/11 None
SNAP00000003641 ENSEMBL cdna:SNAP chromosome:CEL140:III:5453162:5459306:1 11/11 None
GENEFINDER00000003649 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5453561:5459306:1 11/11 None
F48E8.4 ENSEMBL cdna:known chromosome:CEL140:III:5453561:5458924:1 gene:F48E8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5453559-5458923(+) 99.35 100.0
Public Domain and Genome References
Gene Title
Ensembl F48E8.4
Entrez Gene 185984 Entrez gene
SwissProt Q09396 EMBL-EBI
WormBase CE37377 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46544 Hypothetical protein F48E8.4 [Caenorhabditis elegans] ref|NP_498163.2| F48E8.4 [Caenorhabditis elegans] 0.0
blast CAE61351 Hypothetical protein CBG05191 [Caenorhabditis briggsae] 0.0
blast NP_808282 amplified in osteosarcoma [Mus musculus] gb|AAH31768.1| Hypothetical protein 1300007E20 [Mus musculus] 4.0E-13
Sequence
Target Sequence 
>C. ELEGANS:180579_AT
tatttcacatgaatcttgctcgagatatgagccgatttgctagattgatgactgaagttt
tcgtggtgaaaattcaggactttaccaagtggagaaacgacaaaccaatcgctgagcgat
ttgtggaagacttcatcgtatggccaatgcctcctcatgcaacaactgatgcaaatgaga
acgccttcatcaatcttcacaaagatctcggacctcaattgatactggaagtaaaagaaa
gaatatggaatagtaaagttgagaaataccgtaccgaatattggacaaaagactataaaa
tgcgacgacaggcacaatcaatgcaaggattaatgaaagatcttctttcaatgaaagatg
ataaagatgcttttgtttctgttggttctgccacatcaactacaactaaacaggcatctt
tcgatgaacggttactca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTTCACATGAATCTTGCTCGAGA 238 661 1874 Antisense
AGCCGATTTGCTAGATTGATGACTG 340 81 1903 Antisense
AACGACAAACCAATCGCTGAGCGAT 308 145 1969 Antisense
TTCATCGTATGGCCAATGCCTCCTC 44 689 2005 Antisense
TGAGAACGCCTTCATCAATCTTCAC 121 569 2049 Antisense
TCTTCACAAAGATCTCGGACCTCAA 294 615 2067 Antisense
GATCTCGGACCTCAATTGATACTGG 340 415 2077 Antisense
GCGACGACAGGCACAATCAATGCAA 526 287 2175 Antisense
GATGCTTTTGTTTCTGTTGGTTCTG 687 411 2239 Antisense
TTGGTTCTGCCACATCAACTACAAC 489 707 2255 Antisense
GCATCTTTCGATGAACGGTTACTCA 613 305 2287 Antisense
Sequence Source Affymetrix Proprietary Database