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[180576_at]

Description F10C5.2 /REP_DB=WormBase Gene ID /WP=CE26889 /TR=Q19293 /GB=AAK68877.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_high_8_8 > 53      6grp_highest_nonorm > 90      6grp_total > 94      4grp_total > 59      6grp_cgc > 89      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 142.7
(-3)
P 		195.4
(3)
P 		177.2
(0.9)
P 		144.2
(-2.8)
P 		53
B 184.5
(1.3)
P 		213.3
(3)
P 		194.4
(1.9)
P 		110.8
(-3)
P 		103
C 143.5
(0.9)
P 		167.4
(2.4)
P 		178.4
(3)
P 		76.5
(-3)
M 		102
avr 156.9
(0.4) 		192
(3) 		183.3
(2.4) 		110.5
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 121.8
(-2.5)
P 		166.9
(-0.2)
P 		112.8
(-3)
P 		185
(0.8)
P 		228.2
(3)
P 		164.2
(-0.3)
P 		115
B 141.9
(-0)
P 		136.9
(-0.3)
P 		100
(-2.7)
P 		94.8
(-3)
P 		189.8
(3)
P 		153.1
(0.7)
P 		95
C 158.7
(0.3)
P 		134.1
(-1)
P 		138.9
(-0.7)
P 		96.1
(-3)
P 		208.7
(3)
P 		173.8
(1.1)
P 		113
avr 140.8
(-1.5) 		146
(-1.1) 		117.2
(-3) 		125.3
(-2.5) 		208.9
(3) 		163.7
(0) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 51.9
(3)
P 		42.4
(1.1)
P 		46.3
(1.9)
P 		21.1
(-3)
P 		31
B 58.8
(3)
P 		52.7
(2)
P 		45
(0.6)
P 		23.6
(-3)
P 		35
C 47.5
(1.2)
P 		48.1
(1.3)
P 		62.2
(3)
P 		12.9
(-3)
M 		49
avr 52.7
(3) 		47.7
(2.1) 		51.2
(2.7) 		19.2
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 22.5
(-3)
P 		59.4
(1.8)
P 		24.6
(-2.7)
P 		46.9
(0.2)
P 		68.9
(3)
P 		40
(-0.7)
P 		46
B 49.4
(-0.1)
P 		45.6
(-0.6)
P 		24.3
(-3)
P 		25.1
(-2.9)
P 		77.1
(3)
P 		57.1
(0.7)
P 		53
C 58.8
(1.9)
P 		40.9
(-0.3)
P 		35.3
(-1)
P 		19.3
(-3)
P 		67.5
(3)
P 		58.2
(1.8)
P 		48
avr 43.6
(-0.8) 		48.6
(-0.1) 		28.1
(-3) 		30.4
(-2.7) 		71.2
(3) 		51.8
(0.3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180576_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8314
Sequence Type Exemplar sequence
Representative Public ID F10C5.2 NCBI
Target Description F10C5.2 /REP_DB=WormBase Gene ID /WP=CE26889 /TR=Q19293 /GB=AAK68877.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064802(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064802 NCBI Caenorhabditis elegans Metaphase-to-Anaphase Transition defect family member (mat-3) (mat-3) mRNA, complete cds. 11/11 A 
SNAP00000005005 ENSEMBL cdna:SNAP chromosome:CEL140:III:475453:482236:1 11/11 None
GENEFINDER00000005006 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:475453:482236:1 11/11 None
F10C5.1.1 ENSEMBL cdna:known chromosome:CEL140:III:475452:482305:1 gene:F10C5.1 11/11 A 
F10C5.1.2 ENSEMBL cdna:known chromosome:CEL140:III:475453:482305:1 gene:F10C5.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:473601-477046(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mat-3
Ensembl F10C5.1
Entrez Gene 175205 Entrez gene
SwissProt Q19294 EMBL-EBI
WormBase CE26888 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68876 Metaphase-to-anaphase transition defect protein 3 [Caenorhabditis elegans] ref|NP_497203.1| Metaphase-to-Anaphase Transition defect family member (mat-3) [Caenorhabditis elegans] 0.0
blast CAE67168 Hypothetical protein CBG12598 [Caenorhabditis briggsae] 0.0
blast CAE69171 Hypothetical protein CBG15203 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001440 EMBL-EBI TPR repeat 2.7E-4
Pfam IPR001440 EMBL-EBI TPR repeat 3.0E-5
Pfam IPR001440 EMBL-EBI TPR repeat 1.1E-5
Pfam IPR001440 EMBL-EBI TPR repeat 0.013
Pfam IPR001440 EMBL-EBI TPR repeat 0.016
Pfam IPR001440 EMBL-EBI TPR repeat 3.0E-5
Pfam IPR001440 EMBL-EBI TPR repeat 1.1E-5
Pfam IPR001440 EMBL-EBI TPR repeat 0.013
Pfam IPR001440 EMBL-EBI TPR repeat 2.7E-4
Pfam IPR001440 EMBL-EBI TPR repeat 3.0E-5
Pfam IPR001440 EMBL-EBI TPR repeat 1.1E-5
Pfam IPR001440 EMBL-EBI TPR repeat 0.013
Sequence
Target Sequence 
>C. ELEGANS:180576_AT
gacaaacgagattttggcccacctacctgataaatggtgtgctccgaacacgctaaacct
ctacaatcaagtcagcgagcttgtcctcgataacacgcgttcaccaatggcgtcacccgc
ttcgaatagcctttacgcacccggagaagatcaaatgccgactgtaaaacggaatcatac
gtcgagatttgctcagtcgttgatcaaaaataaggagtttagaagggcggcgtttttctt
ggagaaaacgatgaatggcaataaattggatcattttttacattttagatgcttattttt
ggcttattatcaggaacatcttgagaatgacgctgaaggaatcgagagaaaaaccagttt
tgctgaagagagatctccattttcgttgttatatcagcgcatggaagacaaaaagcttcg
agaaaacgaagatgtgtggtttgaatacctaatgggacttctcgaagtggaacttggact
caaagatttggcagaaaagagtttccgaaatgttgtgatccgtgagccccgaatctggcc
agcatgggaagccttgtctcgattaattgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAACGAGATTTTGGCCCACCTA 214 365 972 Antisense
TAAATGGTGTGCTCCGAACACGCTA 178 639 1002 Antisense
GAACACGCTAAACCTCTACAATCAA 704 351 1017 Antisense
GCGAGCTTGTCCTCGATAACACGCG 464 289 1046 Antisense
TAACACGCGTTCACCAATGGCGTCA 612 635 1062 Antisense
CCCGCTTCGAATAGCCTTTACGCAC 200 257 1087 Antisense
GTCGAGATTTGCTCAGTCGTTGATC 561 473 1152 Antisense
GAGAGATCTCCATTTTCGTTGTTAT 524 395 1339 Antisense
CGAAATGTTGTGATCCGTGAGCCCC 514 251 1477 Antisense
GAATCTGGCCAGCATGGGAAGCCTT 464 329 1502 Antisense
GGGAAGCCTTGTCTCGATTAATTGC 436 489 1517 Antisense
Sequence Source Affymetrix Proprietary Database