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[180561_at]

Description W04B5.2 /REP_DB=WormBase Gene ID /WP=CE20139 /TR=Q9UA60 /GB=AAC68915.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 747
1 2 3 4 range
A 101.3
(-2.4)
A 		25.9
(-3)
A 		80.8
(-2.6)
A 		772.6
(3)
P 		747
B 105.4
(-2.7)
A 		73.8
(-3)
A 		76.5
(-3)
A 		651.2
(3)
A 		577
C 119.4
(-2.5)
A 		107.2
(-2.6)
A 		61.9
(-3)
A 		701.2
(3)
P 		639
avr 108.7
(-2.6) 		69
(-3) 		73.1
(-3) 		708.3
(3) 		639

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 93.1
(-3)
A 		120.6
(-2.2)
A 		140.5
(-1.7)
P 		221
(0.5)
P 		200.1
(-0)
M 		310.3
(3)
P 		217
B 89.8
(-2.8)
A 		119.5
(-1.7)
A 		84.2
(-3)
A 		149.3
(-0.6)
A 		183.9
(0.6)
M 		249
(3)
P 		165
C 87.6
(-3)
A 		111.3
(-2.2)
P 		100.5
(-2.6)
A 		192.8
(0.4)
M 		165.3
(-0.5)
A 		274.4
(3)
P 		187
avr 90.2
(-3) 		117.1
(-2.1) 		108.4
(-2.4) 		187.7
(0.1) 		183.1
(-0) 		277.9
(3) 		188
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 36.9
(-1.3)
A 		5.6
(-3)
A 		21.1
(-2.1)
A 		113.1
(3)
P 		108
B 33.6
(-2.2)
A 		18.2
(-3)
A 		17.7
(-3)
A 		138.8
(3)
A 		121
C 39.5
(-1.9)
A 		30.8
(-2.4)
A 		21.6
(-3)
A 		118.2
(3)
P 		97
avr 36.7
(-1.9) 		18.2
(-3) 		20.1
(-2.9) 		123.4
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 17.2
(-3)
A 		42.9
(-0.4)
A 		30.6
(-1.6)
P 		56.1
(1)
P 		60.4
(1.4)
M 		75.5
(3)
P 		58
B 31.2
(-2.1)
A 		39.8
(-1.4)
A 		20.5
(-3)
A 		39.5
(-1.4)
A 		74.7
(1.5)
M 		92.9
(3)
P 		72
C 32.5
(-2.4)
A 		34
(-2.2)
P 		25.6
(-3)
A 		38.8
(-1.8)
M 		53.5
(-0.5)
A 		91.9
(3)
P 		66
avr 27
(-2.9) 		38.9
(-1.7) 		25.6
(-3) 		44.8
(-1.1) 		62.9
(0.7) 		86.8
(3) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180561_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6811
Sequence Type Exemplar sequence
Representative Public ID W04B5.2 NCBI
Target Description W04B5.2 /REP_DB=WormBase Gene ID /WP=CE20139 /TR=Q9UA60 /GB=AAC68915.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065152(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065152 NCBI Caenorhabditis elegans W04B5.2 (W04B5.2) mRNA, complete cds. 11/11 None
W04B5.2 ENSEMBL cdna:known chromosome:CEL140:III:2424343:2427460:1 gene:W04B5.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019372 4/11 Cross Hyb Matching Probes None
GENEFINDER00000019383 4/11 Cross Hyb Matching Probes None
SNAP00000019373 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2424342-2427460(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W04B5.2
Entrez Gene 189182 Entrez gene
SwissProt Q9UA60 EMBL-EBI
WormBase CE20139 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68915 Hypothetical protein W04B5.2 [Caenorhabditis elegans] ref|NP_497553.1| W04B5.2 [Caenorhabditis elegans] 0.0
blast CAB04425 Hypothetical protein F49B2.3 [Caenorhabditis elegans] ref|NP_493500.1| F49B2.3 [Caenorhabditis elegans] 4.0E-15
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497553.1 2 23-45,66-88
W04B5.2 2 23-45,66-88
Sequence
Target Sequence 
>C. ELEGANS:180561_AT
caatgacccgggtgatgagctgccccgtctacctggctcgctacaacgactttggccaac
tgcagcaacacgtgttggaggagcaccccccacgacttcacgcgggttccatggtgcaca
cggcaccccatgtgctccgggactggatccggctcgtgccactcccgaagccagcagcac
cagcggcgccatctgcaacttctcattgcgacattgtgacattattaattttattctttt
tattatcattattgccaataaatacaaaaagtgttacaaaaattttttgctaataatata
ctcgttactcaactctttaactcatttttcaagtactaattgtgaaaattttgaaaaaaa
aaattctttcagaaggcacaaggacatggatggctatgaatgtagacgaggaatatccga
taccaagtgtcaatgccatcttttacaagacatatgtagtcttgtatctaatgattgcga
ggacaataccagatttgcggcttccaagacaatattaataatcgcaacagaaacactcag
caagtgtaggataagtgcattggaccaatcaaaggatccggtgaggctaaacatgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGACCCGGGTGATGAGCTGCCC 71 183 444 Antisense
TGGCCAACTGCAGCAACACGTGTTG 657 543 496 Antisense
CGGGTTCCATGGTGCACACGGCACC 646 239 546 Antisense
CCATGTGCTCCGGGACTGGATCCGG 313 271 571 Antisense
AATATACTCGTTACTCAACTCTTTA 80 173 738 Antisense
GAAGGCACAAGGACATGGATGGCTA 562 331 815 Antisense
ATATCCGATACCAAGTGTCAATGCC 15 21 856 Antisense
GTGTCAATGCCATCTTTTACAAGAC 242 485 870 Antisense
GACAATACCAGATTTGCGGCTTCCA 285 365 925 Antisense
ATCGCAACAGAAACACTCAGCAAGT 321 37 964 Antisense
GGATCCGGTGAGGCTAAACATGCCA 625 511 1017 Antisense
Sequence Source Affymetrix Proprietary Database