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[180546_at]

Description F40A3.4 /REP_DB=WormBase Gene ID /WP=CE10148 /TR=O16263 /GB=AAB65323.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 13      4grp_high_8_8 > 41      6grp_total > 13      4grp_total > 46      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 603
1 2 3 4 range
A 732.4
(3)
P 		274
(-2.1)
P 		212.2
(-2.8)
P 		197.5
(-3)
P 		535
B 330.8
(3)
P 		327.7
(2.9)
P 		245.7
(0.3)
P 		145.1
(-3)
P 		186
C 686.9
(3)
P 		309.6
(-1.1)
P 		213.2
(-2.1)
P 		129.9
(-3)
A 		557
avr 583.4
(3) 		303.8
(-0.9) 		223.7
(-2.1) 		157.5
(-3) 		426

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 570
1 2 3 4 5 6 range
A 284
(-0.6)
P 		601.5
(3)
P 		394.7
(0.6)
P 		357.1
(0.2)
P 		202.5
(-1.6)
P 		75.8
(-3)
A 		526
B 645.6
(3)
P 		557.3
(1.9)
P 		256
(-1.6)
P 		326.3
(-0.8)
P 		141.2
(-3)
P 		150.5
(-2.9)
P 		504
C 644.3
(3)
P 		349.7
(-0.6)
P 		527.2
(1.6)
P 		399.1
(0)
P 		153
(-3)
P 		200.7
(-2.4)
A 		491
avr 524.6
(3) 		502.8
(2.7) 		392.6
(0.9) 		360.8
(0.4) 		165.6
(-2.6) 		142.3
(-3) 		382
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 266.7
(3)
P 		59.5
(-2.2)
P 		55.5
(-2.3)
P 		28.9
(-3)
P 		238
B 105.5
(3)
P 		80.9
(1)
P 		56.9
(-0.9)
P 		30.9
(-3)
P 		75
C 227.4
(3)
P 		89
(-1)
P 		74.3
(-1.5)
P 		21.9
(-3)
A 		206
avr 199.9
(3) 		76.5
(-1.3) 		62.2
(-1.8) 		27.2
(-3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 52.4
(-2)
P 		213.9
(3)
P 		86
(-0.9)
P 		90.6
(-0.8)
P 		61.1
(-1.7)
P 		18.5
(-3)
A 		195
B 224.6
(3)
P 		185.5
(1.6)
P 		62.2
(-2.8)
P 		86.4
(-1.9)
P 		57.4
(-3)
P 		56.1
(-3)
P 		169
C 238.8
(3)
P 		106.7
(-1.2)
P 		134.2
(-0.3)
P 		80.3
(-2)
P 		49.5
(-3)
P 		67.2
(-2.4)
A 		189
avr 171.9
(3) 		168.7
(2.8) 		94.1
(-0.7) 		85.8
(-1.1) 		56
(-2.6) 		47.3
(-3) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180546_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11741
Sequence Type Exemplar sequence
Representative Public ID F40A3.4 NCBI
Target Description F40A3.4 /REP_DB=WormBase Gene ID /WP=CE10148 /TR=O16263 /GB=AAB65323.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072628(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072628 NCBI Caenorhabditis elegans F40A3.4 (F40A3.4) mRNA, complete cds. 9/11 None
F40A3.4 ENSEMBL cdna:known chromosome:CEL140:V:7878348:7879336:1 gene:F40A3.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025355 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7878441-7879296(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40A3.4
Entrez Gene 185507 Entrez gene
SwissProt O16263 EMBL-EBI
WormBase CE33643 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65323 Hypothetical protein F40A3.4 [Caenorhabditis elegans] ref|NP_505029.2| F40A3.4 [Caenorhabditis elegans] 6.0E-35
blast CAE73161 Hypothetical protein CBG20554 [Caenorhabditis briggsae] 6.0E-29
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505029.2 1 46-68
F40A3.4 1 46-68
Sequence
Target Sequence 
>C. ELEGANS:180546_AT
gatagttatagttcatcgattcgtgaggagcagaaagccattcttaatggtcttttaggt
attctttttgcggacggaataatctatgacaactatgacacaactaggaaaattcatcaa
accgtcgatggaatctatcgaatggtcgtcgccttattgctcatcgtgatagctctcatc
atcattgccgtatttctcatttgtttctttcattgtcgccgtgccaacaacgaggagaga
tctccactgcactcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAGTTATAGTTCATCGATTCGTG 710 423 25 Antisense
GAAAGCCATTCTTAATGGTCTTTTA 270 359 57 Antisense
TGGTCTTTTAGGTATTCTTTTTGCG 219 553 72 Antisense
GCGGACGGAATAATCTATGACAACT 652 293 94 Antisense
GAATCTATCGAATGGTCGTCGCCTT 339 327 155 Antisense
GCCTTATTGCTCATCGTGATAGCTC 293 281 175 Antisense
CGTGATAGCTCTCATCATCATTGCC 447 245 189 Antisense
CATTGCCGTATTTCTCATTTGTTTC 527 217 207 Antisense
CTCATTTGTTTCTTTCATTGTCGCC 636 229 220 Antisense
TGTCGCCGTGCCAACAACGAGGAGA 621 557 238 Antisense
CGAGGAGAGATCTCCACTGCACTCG 71 251 255 Antisense
Sequence Source Affymetrix Proprietary Database