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[180543_at]

Description F45F2.7 /REP_DB=WormBase Gene ID /WP=CE10530 /TR=Q22976 /GB=AAC48029.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 05      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 43.9
(-1.6)
A 		22
(-2.4)
A 		7.4
(-3)
A 		162.9
(3)
P 		156
B 55.5
(-1.7)
P 		35.8
(-3)
A 		38.3
(-2.8)
A 		123.9
(3)
P 		88
C 100.5
(0.9)
A 		27.2
(-2.2)
A 		6.7
(-3)
A 		152.1
(3)
P 		145
avr 66.6
(-0.7) 		28.3
(-2.5) 		17.5
(-3) 		146.3
(3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 95.3
(3)
A 		32.3
(-1.3)
A 		87.8
(2.5)
P 		44
(-0.5)
A 		7.5
(-3)
A 		32.4
(-1.3)
A 		88
B 86.9
(3)
P 		67.5
(0.9)
A 		63
(0.4)
A 		31.4
(-3)
A 		39.5
(-2.1)
A 		34.6
(-2.7)
A 		56
C 97.9
(3)
P 		89.1
(2.4)
A 		51.1
(-0.4)
A 		51.5
(-0.3)
A 		24.5
(-2.3)
A 		14.3
(-3)
A 		84
avr 93.4
(3) 		63
(0.4) 		67.3
(0.8) 		42.3
(-1.4) 		23.8
(-3) 		27.1
(-2.7) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 16
(0.8)
A 		4.8
(-2.2)
A 		1.9
(-3)
A 		23.9
(3)
P 		22
B 17.7
(0)
P 		8.8
(-3)
A 		8.9
(-3)
A 		26.4
(3)
P 		18
C 33.3
(3)
A 		7.8
(-1.9)
A 		2.3
(-3)
A 		25.6
(1.5)
P 		31
avr 22.3
(2.1) 		7.1
(-2.2) 		4.4
(-3) 		25.3
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 17.6
(2.5)
A 		11.5
(0.3)
A 		19.1
(3)
P 		11.2
(0.2)
A 		2.3
(-3)
A 		7.9
(-1)
A 		17
B 30.2
(3)
P 		22.5
(0.9)
A 		15.3
(-1.1)
A 		8.3
(-3)
A 		16.1
(-0.9)
A 		12.9
(-1.7)
A 		22
C 36.3
(3)
P 		27.2
(1.3)
A 		13
(-1.4)
A 		10.3
(-2)
A 		7.9
(-2.4)
A 		4.8
(-3)
A 		32
avr 28
(3) 		20.4
(0.7) 		15.8
(-0.8) 		9.9
(-2.6) 		8.8
(-2.9) 		8.5
(-3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180543_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11417
Sequence Type Exemplar sequence
Representative Public ID F45F2.7 NCBI
Target Description F45F2.7 /REP_DB=WormBase Gene ID /WP=CE10530 /TR=Q22976 /GB=AAC48029.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072793(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072793 NCBI Caenorhabditis elegans F45F2.7 (F45F2.7) mRNA, complete cds. 11/11 None
SNAP00000030768 ENSEMBL cdna:SNAP chromosome:CEL140:V:8524348:8527144:1 11/11 None
GENEFINDER00000030781 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8524348:8527144:1 11/11 None
F45F2.7 ENSEMBL cdna:known chromosome:CEL140:V:8524348:8527144:1 gene:F45F2.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8524348-8527145(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45F2.7
Entrez Gene 185808 Entrez gene
SwissProt Q22976 EMBL-EBI
WormBase CE10530 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48029 Hypothetical protein F45F2.7 [Caenorhabditis elegans] ref|NP_505194.1| F45F2.7 [Caenorhabditis elegans] 0.0
blast CAE58382 Hypothetical protein CBG01511 [Caenorhabditis briggsae] 0.0
blast CAA15637 Hypothetical protein H19J13.1 [Caenorhabditis elegans] emb|CAA93534.1| Hypothetical protein H19J13.1 [Caenorhabditis elegans] ref|NP_510045.1| H19J13.1 [Caenorhabditis elegans] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505194.1 11 61-83,98-120,159-181,191-213,225-247,337-359,388-410,420-442,462-484,494-516,536-555
F45F2.7 11 61-83,98-120,159-181,191-213,225-247,337-359,388-410,420-442,462-484,494-516,536-555
SNAP00000030768 11 61-83,98-120,159-181,191-213,225-247,337-359,388-410,420-442,462-484,494-516,536-555
GENEFINDER00000030781 11 61-83,98-120,159-181,191-213,225-247,337-359,388-410,420-442,462-484,494-516,536-555
Sequence
Target Sequence 
>C. ELEGANS:180543_AT
taattctattctgcgtcacgttattggcaactatttttggagcatggagaatgcgagtac
tccaatatcgtcttcatgctcatggggaagttattgatgaaattcttttaattatcggat
tagtcggagaactaatatattgttccattggatttgatatgattgttaatgggaatcgaa
ctggaagtcatgttccgaatctaccaatcgctgtattcacatttcgaataattcaagtaa
taattcaagcaatgtacatattaattgcatctcgtcttcgatgcttatcagaagtcaatg
ctcgaactcaacctggaaaacaaacactgacgtttttagttttaatcaacataactctat
ttgtctatcacactttcgaaggaatgaagtcaacatacggatttccagctctcatggaaa
caaagtactttgagcttttgaatatatcgtcaccattggttgttttctaccgattccata
gttcagcttgtttggcagaaatatggaagcatgcctattctacaaaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATTCTATTCTGCGTCACGTTATT 555 633 1292 Antisense
GAGTACTCCAATATCGTCTTCATGC 647 399 1346 Antisense
GTCATGTTCCGAATCTACCAATCGC 304 467 1478 Antisense
TACCAATCGCTGTATTCACATTTCG 656 649 1493 Antisense
GTACATATTAATTGCATCTCGTCTT 51 461 1545 Antisense
ATCTCGTCTTCGATGCTTATCAGAA 176 33 1560 Antisense
TCAATGCTCGAACTCAACCTGGAAA 62 633 1586 Antisense
ACTCTATTTGTCTATCACACTTTCG 156 101 1645 Antisense
ACATACGGATTTCCAGCTCTCATGG 520 105 1684 Antisense
GTCACCATTGGTTGTTTTCTACCGA 576 461 1740 Antisense
GGAAGCATGCCTATTCTACAAAACA 177 531 1796 Antisense
Sequence Source Affymetrix Proprietary Database