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[180526_at]

Description B0524.2 /REP_DB=WormBase Gene ID /WP=CE20464 /TR=Q9N5Z7 /GB=AAF39747.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 31      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 5.4
(3)
A 		3.5
(-1.6)
A 		2.9
(-3)
A 		5
(2)
A 		3
B 7.1
(-0.8)
A 		7.9
(0)
A 		10.8
(3)
A 		5
(-3)
A 		6
C 26.5
(3)
A 		3.9
(-3)
A 		4.2
(-2.9)
A 		18.4
(0.8)
A 		23
avr 13
(3) 		5.1
(-3) 		6
(-2.3) 		9.5
(0.3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 2.8
(-2.8)
A 		2.2
(-2.9)
A 		1.8
(-3)
A 		5.7
(-2.3)
A 		6.6
(-2.2)
A 		36
(3)
A 		34
B 10.2
(2.9)
A 		3.1
(-3)
A 		5.2
(-1.3)
A 		4.1
(-2.2)
A 		10.3
(3)
A 		8
(1.1)
A 		7
C 2.4
(-3)
A 		7.5
(-1.9)
A 		4
(-2.6)
A 		2.3
(-3)
A 		29.9
(3)
A 		4.8
(-2.5)
A 		28
avr 5.1
(-2.3) 		4.3
(-2.7) 		3.7
(-3) 		4
(-2.9) 		15.6
(2.7) 		16.3
(3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2
(3)
A 		0.8
(-2.5)
A 		0.7
(-3)
A 		0.7
(-3)
A 		1
B 2.2
(1.7)
A 		1.9
(0.4)
A 		2.5
(3)
A 		1.1
(-3)
A 		1
C 8.8
(3)
A 		1.1
(-3)
A 		1.5
(-2.7)
A 		3.1
(-1.4)
A 		8
avr 4.3
(3) 		1.3
(-3) 		1.6
(-2.4) 		1.6
(-2.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 0.5
(-2.9)
A 		0.8
(-2.7)
A 		0.4
(-3)
A 		1.4
(-2.3)
A 		2
(-1.9)
A 		8.8
(3)
A 		8
B 3.5
(1.7)
A 		1
(-3)
A 		1.3
(-2.4)
A 		1.1
(-2.8)
A 		4.2
(3)
A 		3
(0.8)
A 		3
C 0.9
(-2.7)
A 		2.3
(-1.8)
A 		1
(-2.7)
A 		0.5
(-3)
A 		9.7
(3)
A 		1.6
(-2.3)
A 		9
avr 1.6
(-2) 		1.4
(-2.3) 		0.9
(-3) 		1
(-2.9) 		5.3
(3) 		4.5
(1.9) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180526_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8342
Sequence Type Exemplar sequence
Representative Public ID B0524.2 NCBI
Target Description B0524.2 /REP_DB=WormBase Gene ID /WP=CE20464 /TR=Q9N5Z7 /GB=AAF39747.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065073(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065073 NCBI Caenorhabditis elegans B0524.2 (B0524.2) mRNA, complete cds. 11/11 None
SNAP00000041791 ENSEMBL cdna:SNAP chromosome:CEL140:III:1888074:1917217:1 11/11 None
GENEFINDER00000041798 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1894091:1899845:1 11/11 None
B0524.2 ENSEMBL cdna:known chromosome:CEL140:III:1895418:1899845:1 gene:B0524.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1895417-1899845(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0524.2
Entrez Gene 182024 Entrez gene
SwissProt Q9N5Z7 EMBL-EBI
WormBase CE20464 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
45735 nutrient reservoir activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39746 Hypothetical protein B0524.3 [Caenorhabditis elegans] ref|NP_497472.2| B0524.3 [Caenorhabditis elegans] 0.0
blast AAF39747 Hypothetical protein B0524.2 [Caenorhabditis elegans] ref|NP_497474.1| B0524.2 [Caenorhabditis elegans] 1.0E-138
blast AAF39747 Hypothetical protein B0524.2 [Caenorhabditis elegans] ref|NP_497474.1| B0524.2 [Caenorhabditis elegans] 1.0E-126
blast CAE69132 Hypothetical protein CBG15159 [Caenorhabditis briggsae] 1.0E-123
blast AAF39747 Hypothetical protein B0524.2 [Caenorhabditis elegans] ref|NP_497474.1| B0524.2 [Caenorhabditis elegans] 1.0E-120
blast CAE69132 Hypothetical protein CBG15159 [Caenorhabditis briggsae] 1.0E-120
blast CAE69132 Hypothetical protein CBG15159 [Caenorhabditis briggsae] 6.0E-97
blast CAA98440 Hypothetical protein D1054.1 [Caenorhabditis elegans] ref|NP_505749.3| D1054.1 [Caenorhabditis elegans] 1.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 5.5E-8
Pfam IPR002641 EMBL-EBI Patatin 8.9E-18
Pfam IPR002641 EMBL-EBI Patatin 8.9E-18
Sequence
Target Sequence 
>C. ELEGANS:180526_AT
acaaaacgctcggcgcaatgtctccgggattcgttatcagtgatcgtttgcggaaagtcg
tcgaaaaattcctacctgatgacgtggcaaaggcaaacgatctgctttttgtgtcggtca
ccaagttgaagacatggaaaaacgagctgatctcgaatttcgagtcgaaaatcgatttga
tcgattgtctgatggcgagctgctatcatccaatgtactcgtcgggtttgaacggaaaag
cgccgattcttcggggagaggcatacatcgacggtggctactcgaacatgattccagagt
tccttgacatgcgtacggtcacagtgtctgcatttgccggtgatgcggacatttgtcccg
tcgagaaatctgcattgttcaatgattttatgttcgcattctttaatcagaatatgaagc
tcactctgtccaacatgaaacgcgtcacaaatgcactttttccgccgacacgtgccattc
ttcaggagtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAAACGCTCGGCGCAATGTCTCC 519 111 260 Antisense
ATGTCTCCGGGATTCGTTATCAGTG 1 49 277 Antisense
GATTGTCTGATGGCGAGCTGCTATC 597 433 442 Antisense
ATCATCCAATGTACTCGTCGGGTTT 496 29 464 Antisense
GAACGGAAAAGCGCCGATTCTTCGG 560 345 489 Antisense
GAGGCATACATCGACGGTGGCTACT 524 405 517 Antisense
AGAGTTCCTTGACATGCGTACGGTC 3 59 555 Antisense
CGGTCACAGTGTCTGCATTTGCCGG 130 245 575 Antisense
GATGCGGACATTTGTCCCGTCGAGA 147 415 601 Antisense
ATATGAAGCTCACTCTGTCCAACAT 351 19 671 Antisense
GACACGTGCCATTCTTCAGGAGTAT 578 367 726 Antisense
Sequence Source Affymetrix Proprietary Database