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[180520_at]

Description T28H10.2 /REP_DB=WormBase Gene ID /WP=CE06530 /TR=Q22857 /GB=CAA99934.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 32      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 52      6grp_total > 30      4grp_total > 52      6grp_highest_sub > 02_03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 766
1 2 3 4 range
A 600.4
(1.6)
P 		754.5
(3)
P 		590.5
(1.5)
P 		91.3
(-3)
P 		663
B 804.4
(2.9)
P 		815.6
(3)
P 		718.2
(2.2)
P 		49.5
(-3)
P 		766
C 761.1
(3)
P 		632.8
(1.9)
P 		712
(2.6)
P 		61.1
(-3)
P 		700
avr 722
(2.9) 		734.3
(3) 		673.6
(2.5) 		67.3
(-3) 		667

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 759
1 2 3 4 5 6 range
A 460.7
(-0.1)
P 		767.6
(2.6)
P 		808.3
(3)
P 		624
(1.4)
P 		337.7
(-1.1)
P 		126.1
(-3)
P 		682
B 459.9
(-0.5)
P 		884.8
(3)
P 		818
(2.5)
P 		660.8
(1.2)
P 		408.7
(-0.9)
P 		155.7
(-3)
P 		729
C 513.8
(-0.1)
P 		877.3
(3)
P 		746.1
(1.9)
P 		706
(1.5)
P 		312.4
(-1.8)
P 		171.2
(-3)
P 		706
avr 478.1
(-0.2) 		843.2
(3) 		790.8
(2.5) 		663.6
(1.4) 		352.9
(-1.2) 		151
(-3) 		692
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 218.6
(3)
P 		163.8
(1.4)
P 		154.4
(1.1)
P 		13.4
(-3)
P 		205
B 256.5
(3)
P 		201.4
(1.7)
P 		166.4
(0.8)
P 		10.6
(-3)
P 		246
C 252
(3)
P 		181.9
(1.3)
P 		248.1
(2.9)
P 		10.3
(-3)
P 		242
avr 242.4
(3) 		182.4
(1.4) 		189.6
(1.6) 		11.4
(-3) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 264
1 2 3 4 5 6 range
A 84.9
(-1.7)
P 		273
(3)
P 		176
(0.6)
P 		158.3
(0.2)
P 		101.9
(-1.2)
P 		30.7
(-3)
P 		242
B 160
(-0.4)
P 		294.5
(3)
P 		198.6
(0.6)
P 		175
(-0)
P 		166.1
(-0.3)
P 		58.1
(-3)
P 		236
C 190.4
(0.8)
P 		267.7
(3)
P 		189.9
(0.8)
P 		142
(-0.6)
P 		101.1
(-1.8)
P 		57.3
(-3)
P 		210
avr 145.1
(-0.5) 		278.4
(3) 		188.2
(0.6) 		158.4
(-0.1) 		123
(-1.1) 		48.7
(-3) 		230
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180520_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13048
Sequence Type Exemplar sequence
Representative Public ID T28H10.2 NCBI
Target Description T28H10.2 /REP_DB=WormBase Gene ID /WP=CE06530 /TR=Q22857 /GB=CAA99934.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073735(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073735 NCBI Caenorhabditis elegans T28H10.2 (T28H10.2) mRNA, complete cds. 11/11 None
SNAP00000023145 ENSEMBL cdna:SNAP chromosome:CEL140:V:12513300:12514771:-1 11/11 None
GENEFINDER00000023147 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12513300:12514813:-1 11/11 None
T28H10.2 ENSEMBL cdna:known chromosome:CEL140:V:12513456:12514813:-1 gene:T28H10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12513455-12514813(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28H10.2
Entrez Gene 189062 Entrez gene
SwissProt Q22857 EMBL-EBI
WormBase CE06530 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99934 Hypothetical protein T28H10.2 [Caenorhabditis elegans] ref|NP_506136.1| T28H10.2 [Caenorhabditis elegans] 1.0E-114
blast CAE75505 Hypothetical protein CBG23515 [Caenorhabditis briggsae] 1.0E-113
blast CAA99934 Hypothetical protein T28H10.2 [Caenorhabditis elegans] ref|NP_506136.1| T28H10.2 [Caenorhabditis elegans] 1.0E-108
blast CAA99934 Hypothetical protein T28H10.2 [Caenorhabditis elegans] ref|NP_506136.1| T28H10.2 [Caenorhabditis elegans] 1.0E-104
blast CAE75505 Hypothetical protein CBG23515 [Caenorhabditis briggsae] 3.0E-92
blast AAF39761 Hypothetical protein C15F1.1 [Caenorhabditis elegans] ref|NP_495434.1| C15F1.1 [Caenorhabditis elegans] 9.0E-63
blast AAF39761 Hypothetical protein C15F1.1 [Caenorhabditis elegans] ref|NP_495434.1| C15F1.1 [Caenorhabditis elegans] 2.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000023147 1 26-48
Sequence
Target Sequence 
>C. ELEGANS:180520_AT
gcgacggagcatgtgcatcgtgggttgcttcggatcgaggttcatgtcaaagaaaagagt
acatcaagaaaaactgtcgaaaaagttgcggaaattgtcctcgctatgagttaaaattcg
atactcgtcgcttgaatccacaacttcaacctattcaccaattggtgggacgatggaagg
gtgaacataccggaaaagttacatttccaacaattccaactttcaaatacacagaggaag
tcgaaatttcaattcctgaaggaagcaatattcgatcactcaattacactgccgccgctt
ggtctagtgataaagaagatcttcaccgcgaatcgggttacatcatcattaaaccaaaca
ctcgtgaagttattttgacaacagtcatgtccaatggtttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGACGGAGCATGTGCATCGTGGGT 651 287 101 Antisense
TGCATCGTGGGTTGCTTCGGATCGA 391 579 114 Antisense
GCTTCGGATCGAGGTTCATGTCAAA 40 307 127 Antisense
GTTGCGGAAATTGTCCTCGCTATGA 379 439 185 Antisense
ATTCGATACTCGTCGCTTGAATCCA 264 7 216 Antisense
CAACTTCAACCTATTCACCAATTGG 313 189 241 Antisense
TTTCCAACAATTCCAACTTTCAAAT 103 669 304 Antisense
TATTCGATCACTCAATTACACTGCC 513 663 369 Antisense
AAAGAAGATCTTCACCGCGAATCGG 478 121 412 Antisense
CCGCGAATCGGGTTACATCATCATT 276 261 426 Antisense
ACAACAGTCATGTCCAATGGTTTCA 45 113 478 Antisense
Sequence Source Affymetrix Proprietary Database