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[180516_at]

Description F53B1.3 /REP_DB=WormBase Gene ID /WP=CE04643 /TR=Q20695 /GB=AAB52646.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 05      6grp_total > 05      4grp_total > 38      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 233
(3)
P 		107.7
(-1.9)
P 		78.4
(-3)
A 		188.9
(1.3)
P 		155
B 284.8
(3)
P 		107.6
(-2.2)
P 		80.3
(-3)
A 		135.5
(-1.4)
P 		205
C 276.4
(3)
P 		181.4
(0.3)
P 		65.3
(-3)
A 		130.4
(-1.1)
P 		211
avr 264.7
(3) 		132.2
(-1.2) 		74.7
(-3) 		151.6
(-0.6) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 247
(3)
P 		162.5
(0)
P 		144.7
(-0.6)
P 		103.2
(-2.1)
P 		129.1
(-1.2)
P 		77.2
(-3)
P 		170
B 239
(3)
P 		170.4
(0.4)
P 		116.9
(-1.7)
P 		144.6
(-0.6)
P 		112.4
(-1.9)
P 		83.6
(-3)
P 		155
C 256.5
(3)
P 		172.2
(0.3)
P 		160.3
(-0.1)
P 		93.7
(-2.3)
M 		160
(-0.1)
P 		72.5
(-3)
P 		184
avr 247.5
(3) 		168.4
(0.2) 		140.6
(-0.8) 		113.8
(-1.7) 		133.8
(-1) 		77.8
(-3) 		170
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 84.8
(3)
P 		23.4
(-2.7)
P 		20.5
(-3)
A 		27.6
(-2.3)
P 		64
B 90.8
(3)
P 		26.6
(-2.3)
P 		18.6
(-3)
A 		28.9
(-2.1)
P 		72
C 91.5
(3)
P 		52.2
(-0.4)
P 		22.8
(-2.9)
A 		22
(-3)
P 		70
avr 89
(3) 		34.1
(-1.8) 		20.6
(-3) 		26.2
(-2.5) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 45.5
(1.1)
P 		57.8
(3)
P 		31.5
(-1)
P 		26.2
(-1.9)
P 		39
(0.1)
P 		18.8
(-3)
P 		39
B 83.2
(3)
P 		56.7
(0.1)
P 		28.4
(-3)
P 		38.3
(-1.9)
P 		45.7
(-1.1)
P 		31.2
(-2.7)
P 		55
C 95.1
(3)
P 		52.5
(-0.3)
P 		40.8
(-1.3)
P 		18.8
(-3)
M 		51.8
(-0.4)
P 		24.3
(-2.6)
P 		76
avr 74.6
(3) 		55.7
(0.7) 		33.6
(-1.9) 		27.8
(-2.6) 		45.5
(-0.5) 		24.8
(-3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180516_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18456
Sequence Type Exemplar sequence
Representative Public ID F53B1.3 NCBI
Target Description F53B1.3 /REP_DB=WormBase Gene ID /WP=CE04643 /TR=Q20695 /GB=AAB52646.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075987(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075987 NCBI Caenorhabditis elegans F53B1.3 (F53B1.3) mRNA, complete cds. 11/11 None
GENEFINDER00000023000 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2105626:2107065:1 11/11 None
F53B1.3 ENSEMBL cdna:known chromosome:CEL140:X:2105626:2107065:1 gene:F53B1.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022988 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2105625-2107065(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53B1.3
Entrez Gene 186148 Entrez gene
SwissProt Q20695 EMBL-EBI
WormBase CE04643 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52646 Hypothetical protein F53B1.3 [Caenorhabditis elegans] ref|NP_508388.1| F53B1.3 [Caenorhabditis elegans] 4.0E-59
blast CAE68354 Hypothetical protein CBG14087 [Caenorhabditis briggsae] 4.0E-51
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508388.1 1 90-112
F53B1.3 1 90-112
GENEFINDER00000023000 1 90-112
Sequence
Target Sequence 
>C. ELEGANS:180516_AT
ttcacctgctcacgattctagtagtttttgcttggaaatccgaaatagtttcgacacgaa
atgcggttcatacagtgcggcatgatatcgcattcgtgcacacctttgataccagagaga
tgtgtatgtcacgatgtcgcaccggctgcactcaacagcactccgaagacctgatgatgc
cacgttggcactgtccgttgcaggaagaagatgacaatgatgtggaagaggaggagaaga
catcgttgggtggcaagatgctcatcattgttccctgtgtcatatttggatcactccttt
tcttattcttttgtgtttctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACCTGCTCACGATTCTAGTAGT 300 687 23 Antisense
GAAATGCGGTTCATACAGTGCGGCA 488 361 80 Antisense
GCGGCATGATATCGCATTCGTGCAC 519 285 99 Antisense
TCGTGCACACCTTTGATACCAGAGA 244 605 116 Antisense
TGTGTATGTCACGATGTCGCACCGG 436 555 143 Antisense
ACTCAACAGCACTCCGAAGACCTGA 167 101 172 Antisense
AAGACCTGATGATGCCACGTTGGCA 620 153 188 Antisense
TTGGCACTGTCCGTTGCAGGAAGAA 445 707 207 Antisense
TCATCATTGTTCCCTGTGTCATATT 387 621 284 Antisense
TATTTGGATCACTCCTTTTCTTATT 378 661 305 Antisense
TTTTCTTATTCTTTTGTGTTTCTGC 348 677 320 Antisense
Sequence Source Affymetrix Proprietary Database