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[180497_at]

Description C25B8.6 /REP_DB=WormBase Gene ID /WP=CE17465 /TR=Q18150 /GB=AAK39191.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 724
1 2 3 4 range
A 634.9
(2.4)
P 		309.3
(-1.7)
P 		210.7
(-3)
P 		678.4
(3)
P 		468
B 664
(1.1)
P 		228.3
(-2.5)
P 		163.7
(-3)
P 		887.3
(3)
P 		724
C 617
(2.1)
P 		243.3
(-2.8)
P 		228.9
(-3)
P 		684.5
(3)
P 		456
avr 638.6
(1.8) 		260.3
(-2.4) 		201.1
(-3) 		750.1
(3) 		549

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 517.1
(3)
P 		486.1
(2.3)
P 		367.6
(-0.4)
P 		339.9
(-1)
P 		249.7
(-3)
P 		272.3
(-2.5)
P 		267
B 589.6
(3)
P 		545.3
(2.1)
P 		333.6
(-2)
P 		334.3
(-2)
P 		283.4
(-3)
P 		353.4
(-1.6)
P 		306
C 641.4
(3)
P 		407
(-1)
P 		314.9
(-2.6)
P 		289.2
(-3)
P 		313.7
(-2.6)
P 		324.8
(-2.4)
P 		352
avr 582.7
(3) 		479.5
(0.9) 		338.7
(-1.9) 		321.1
(-2.2) 		282.3
(-3) 		316.8
(-2.3) 		300
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 193
1 2 3 4 range
A 231.2
(3)
P 		67.1
(-2.6)
P 		55.1
(-3)
P 		99.3
(-1.5)
P 		176
B 211.7
(3)
P 		56.4
(-2.4)
P 		37.9
(-3)
P 		189.1
(2.2)
P 		174
C 204.3
(3)
P 		70
(-3)
P 		79.8
(-2.6)
P 		115.4
(-1)
P 		134
avr 215.7
(3) 		64.5
(-2.7) 		57.6
(-3) 		134.6
(-0.1) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 95.3
(-1.4)
P 		172.9
(3)
P 		80
(-2.2)
P 		86.2
(-1.9)
P 		75.3
(-2.5)
P 		66.3
(-3)
P 		107
B 205.1
(3)
P 		181.5
(1.9)
P 		81
(-3)
P 		88.6
(-2.6)
P 		115.1
(-1.4)
P 		131.8
(-0.5)
P 		124
C 237.7
(3)
P 		124.2
(-0.8)
P 		80.1
(-2.3)
P 		58.2
(-3)
P 		101.5
(-1.6)
P 		108.7
(-1.3)
P 		180
avr 179.4
(3) 		159.5
(1.8) 		80.4
(-2.8) 		77.7
(-3) 		97.3
(-1.8) 		102.3
(-1.5) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180497_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17196
Sequence Type Exemplar sequence
Representative Public ID C25B8.6 NCBI
Target Description C25B8.6 /REP_DB=WormBase Gene ID /WP=CE17465 /TR=Q18150 /GB=AAK39191.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076715(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076715 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-120) (nhr-120) mRNA, complete cds. 11/11 None
SNAP00000010947 ENSEMBL cdna:SNAP chromosome:CEL140:X:6636764:6638015:-1 11/11 None
C25B8.6 ENSEMBL cdna:known chromosome:CEL140:X:6636514:6640575:-1 gene:C25B8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6636763-6639425(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhr-120
Ensembl C25B8.6
Entrez Gene 182882 Entrez gene
SwissProt Q18150 EMBL-EBI
WormBase CE30877 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
4879 ligand-dependent nuclear receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAR11992 nuclear receptor NHR-120 [Caenorhabditis elegans] gb|AAK39191.2| Nuclear hormone receptor family protein 120 [Caenorhabditis elegans] ref|NP_509116.2| Nuclear Hormone Receptor family member (nhr-120) [Caenorhabditis elegans] 0.0
blast T34118 hypothetical protein C25B8.6 - Caenorhabditis elegans 0.0
blast CAE65766 Hypothetical protein CBG10855 [Caenorhabditis briggsae] 0.0
blast CAE65766 Hypothetical protein CBG10855 [Caenorhabditis briggsae] 1.0E-154
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.0E-37
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.4E-35
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.0E-37
Sequence
Target Sequence 
>C. ELEGANS:180497_AT
ttggatggtggtcgatcttctcttaattgttgaatttgcgaagacttttgaatcattccg
caaactaagtgaatttgatcagaaatgcctgatttcgcacattggtggcgttctccatgt
tgctacacaatcgttctactcatattttgaagccaaatcttctacattgacatttccaga
tggaattaacgcatttgaacggaaaatggctgacgtcgagaaagacgataagaggaaggt
attcttaaactattatgcggagatgtacaatgcaccggttaatgagtttttgaaaaataa
agttacaatgacagaatactcatttttcaaatctatttctcttttctcgcctgataactt
ggaaatgtcagacgctggaagattaattgcaaatgaagagcgggaacgtcttacaactct
tcttcgaaaataccttgttgcagaatatggcttcaaagttggaatgaacagattttcgtt
aatattaatggcaatttccactttcgttaattatggcgagaaacgccgcgattacatttc
tctaatgagcctactgaagtttgatcttgcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGATGGTGGTCGATCTTCTCTTA 204 709 684 Antisense
GCCTGATTTCGCACATTGGTGGCGT 228 283 770 Antisense
GTTGCTACACAATCGTTCTACTCAT 625 439 802 Antisense
AAGCCAAATCTTCTACATTGACATT 267 149 833 Antisense
GGAGATGTACAATGCACCGGTTAAT 530 517 942 Antisense
TCTCTTTTCTCGCCTGATAACTTGG 145 613 1021 Antisense
CGTCTTACAACTCTTCTTCGAAAAT 461 243 1090 Antisense
ATGGCAATTTCCACTTTCGTTAATT 225 51 1171 Antisense
GCGAGAAACGCCGCGATTACATTTC 58 291 1199 Antisense
TACATTTCTCTAATGAGCCTACTGA 105 645 1216 Antisense
AGCCTACTGAAGTTTGATCTTGCGA 108 83 1231 Antisense
Sequence Source Affymetrix Proprietary Database