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[180479_at]

Description ZC477.5 /REP_DB=WormBase Gene ID /WP=CE28164 /TR=Q23342 /GB=AAK19011.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 18      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 89.7
(-3)
A 		121.7
(-1.5)
A 		158.5
(0.2)
M 		219.5
(3)
A 		130
B 123.9
(-0.4)
P 		138.4
(1)
A 		97.8
(-3)
A 		158.8
(3)
M 		61
C 119.6
(-0.3)
A 		137
(1.5)
A 		92.6
(-3)
A 		151.7
(3)
A 		59
avr 111.1
(-3) 		132.4
(-1.1) 		116.3
(-2.5) 		176.7
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 189.2
(3)
A 		132.7
(-0.3)
A 		88
(-3)
A 		147.9
(0.6)
A 		147.4
(0.5)
P 		144.5
(0.3)
P 		101
B 157.9
(-1.7)
P 		152.2
(-3)
A 		174.6
(2)
M 		179
(3)
A 		154.9
(-2.4)
M 		167.9
(0.5)
M 		27
C 158.3
(0.9)
M 		154.7
(0.7)
P 		99.7
(-3)
A 		189.4
(3)
A 		109.8
(-2.3)
A 		184.2
(2.7)
M 		90
avr 168.5
(2.6) 		146.5
(0) 		120.8
(-3) 		172.1
(3) 		137.4
(-1.1) 		165.5
(2.2) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 32.7
(-0.5)
A 		26.4
(-3)
A 		41.4
(3)
M 		32.1
(-0.7)
A 		15
B 39.5
(3)
P 		34.2
(1.1)
A 		22.7
(-3)
A 		33.8
(1)
M 		17
C 39.6
(3)
A 		39.4
(2.9)
A 		32.3
(-0.1)
A 		25.6
(-3)
A 		14
avr 37.3
(3) 		33.3
(-0.5) 		32.1
(-1.6) 		30.5
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 34.9
(0.4)
A 		47.2
(3)
A 		19.2
(-3)
A 		37.5
(0.9)
A 		44.5
(2.4)
P 		35.2
(0.4)
P 		28
B 54.9
(0.7)
P 		50.7
(-0.6)
A 		42.4
(-3)
M 		47.4
(-1.5)
A 		62.9
(3)
M 		62.6
(2.9)
M 		21
C 58.7
(2.5)
M 		47.2
(0.6)
P 		25.4
(-3)
A 		38.1
(-0.9)
A 		35.5
(-1.3)
A 		61.7
(3)
M 		36
avr 49.5
(2.1) 		48.4
(1.8) 		29
(-3) 		41
(-0) 		47.6
(1.6) 		53.2
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180479_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10231
Sequence Type Exemplar sequence
Representative Public ID ZC477.5 NCBI
Target Description ZC477.5 /REP_DB=WormBase Gene ID /WP=CE28164 /TR=Q23342 /GB=AAK19011.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068706
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068706 NCBI Caenorhabditis elegans ZC477.5 (ZC477.5) mRNA, complete cds. A 
GENEFINDER00000039663 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7101447:7103072:-1 None
SNAP00000039646 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7101447:7103513:-1 None
ZC477.5 ENSEMBL cdna:known chromosome:CEL140:IV:7101420:7103245:-1 gene:ZC477.5 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7099368-7103078(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC477.5
Entrez Gene 177483 Entrez gene
SwissProt Q23342 EMBL-EBI
WormBase CE28164 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-173
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-169
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-149
blast T27604 hypothetical protein ZC477.5 - Caenorhabditis elegans 1.0E-147
blast T27604 hypothetical protein ZC477.5 - Caenorhabditis elegans 1.0E-144
blast CAE61801 Hypothetical protein CBG05767 [Caenorhabditis briggsae] 3.0E-62
Sequence
Target Sequence 
>C. ELEGANS:180479_AT
aaataccagagctccaaacattgaaaatgactatgtaccttcaaagcgtgttgtattcgt
taatgaaagagaatcatcgtcaagaatgtcggaaaacgatgtcaaaatgcggctctatgc
agttccagatcatattcaatatgaaataactaaggaacgtcgtgtgctatccgaacctga
aaagaaaaaatcaaaaacgatggcccttctgaacgatcttcttgcgtacggacgccaaaa
ttgtcctggtgtcattgaggagattttgaagaaaccagtgcatgttcccaacgaccactc
tacacaacaaccacagccacctgagcaaccaattcgtcgagttgatattgctgatgaaga
gcacattgctgtttacgttggtgatggtgatgagtgtcgcctccttctgctccaagtgca
ttacacacactgtttcactgtctgcttctctccgatccaagacatttctcgttccgtctg
tctaaatctcttaatgatcggagcgaagccggtagaagaagatgaagctgaagactatga
agaagtatccgaactattcctccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATACCAGAGCTCCAAACATTGAA 573 115 516 Antisense
GACTATGTACCTTCAAAGCGTGTTG 306 373 544 Antisense
AAACGATGTCAAAATGCGGCTCTAT 289 127 609 Antisense
AATGCGGCTCTATGCAGTTCCAGAT 350 167 621 Antisense
TGATGGTGATGAGTGTCGCCTCCTT 198 567 897 Antisense
TGCTCCAAGTGCATTACACACACTG 6 583 923 Antisense
TACACACACTGTTTCACTGTCTGCT 338 643 937 Antisense
TTCTCTCCGATCCAAGACATTTCTC 613 691 961 Antisense
GATCCAAGACATTTCTCGTTCCGTC 15 415 969 Antisense
TTCCGTCTGTCTAAATCTCTTAATG 494 699 987 Antisense
AGAAGTATCCGAACTATTCCTCCGT 283 75 1056 Antisense
Sequence Source Affymetrix Proprietary Database