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[180473_s_at]

Description F52E4.7 /REP_DB=WormBase Gene ID /WP=CE10836 /TR=Q20678 /GB=AAB54035.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 62      6grp_total > 31      4grp_total > 54      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 315
1 2 3 4 range
A 639.4
(3)
P 		493.7
(-0)
P 		383.3
(-2.3)
P 		351.8
(-3)
P 		288
B 599.1
(3)
P 		466
(-0.8)
P 		575.4
(2.3)
P 		389.6
(-3)
P 		210
C 620.3
(3)
P 		438.6
(-0.7)
P 		406.7
(-1.3)
P 		324.8
(-3)
P 		296
avr 619.6
(3) 		466.1
(-0.5) 		455.1
(-0.7) 		355.4
(-3) 		264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 382
1 2 3 4 5 6 range
A 424.3
(-0.2)
P 		534.1
(3)
P 		521.2
(2.6)
P 		421.5
(-0.2)
P 		338.5
(-2.6)
P 		326.2
(-3)
P 		208
B 595.5
(1.1)
P 		555.5
(0.4)
P 		707.7
(3)
P 		505
(-0.5)
P 		366.1
(-2.8)
P 		356
(-3)
P 		352
C 432.7
(-1)
P 		549.3
(3)
P 		449.5
(-0.4)
P 		422
(-1.4)
P 		401.4
(-2.1)
P 		374.5
(-3)
P 		175
avr 484.2
(0.8) 		546.3
(2.6) 		559.5
(3) 		449.5
(-0.2) 		368.7
(-2.5) 		352.2
(-3) 		207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 232.8
(3)
P 		107.2
(-1.2)
P 		100.2
(-1.4)
P 		51.5
(-3)
P 		181
B 191.1
(3)
P 		115.1
(-1.2)
P 		133.3
(-0.2)
P 		83
(-3)
P 		108
C 205.3
(3)
P 		126.1
(-0.2)
P 		141.7
(0.5)
P 		54.8
(-3)
P 		151
avr 209.7
(3) 		116.1
(-0.8) 		125.1
(-0.5) 		63.1
(-3) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 78.2
(-3)
P 		189.9
(3)
P 		113.5
(-1.1)
P 		106.9
(-1.5)
P 		102.1
(-1.7)
P 		79.4
(-2.9)
P 		112
B 207.2
(3)
P 		184.9
(1.2)
P 		171.9
(0.2)
P 		133.8
(-2.9)
P 		148.8
(-1.7)
P 		132.8
(-3)
P 		74
C 160.4
(2.5)
P 		167.6
(3)
P 		114.4
(-0.9)
P 		84.9
(-3)
P 		129.9
(0.3)
P 		125.4
(-0.1)
P 		83
avr 148.6
(0.3) 		180.8
(3) 		133.3
(-0.9) 		108.5
(-3) 		126.9
(-1.5) 		112.5
(-2.7) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180473_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18588
Sequence Type Exemplar sequence
Representative Public ID F52E4.7 NCBI
Target Description F52E4.7 /REP_DB=WormBase Gene ID /WP=CE10836 /TR=Q20678 /GB=AAB54035.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076129(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076129 NCBI Caenorhabditis elegans F52E4.7 (F52E4.7) mRNA, complete cds. 11/11 None
SNAP00000026387 ENSEMBL cdna:SNAP chromosome:CEL140:X:3095662:3099828:-1 11/11 None
GENEFINDER00000026390 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3095662:3099828:-1 11/11 None
F52E4.7 ENSEMBL cdna:known chromosome:CEL140:X:3095439:3099828:-1 gene:F52E4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3095661-3099828(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F52E4.7
Entrez Gene 180598 Entrez gene
SwissProt Q20678 EMBL-EBI
WormBase CE10836 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262430_S_AT expressed protein at
CANINE_2:CFA.18432.1.S1_S_AT similar to Exocyst complex component Sec3 cfa
CANINE_2:CFAAFFX.4161.1.S1_S_AT similar to Exocyst complex component Sec3 cfa
DROSOPHILA_2:1630943_AT dm
DROSGENOME1:141529_AT dm
CHICKEN:GGA.19026.1.S1_S_AT SEC3-like 1 (S. cerevisiae) gga
CHICKEN:GGAAFFX.24277.1.S1_S_AT SEC3-like 1 (S. cerevisiae) gga
HU35KSUBB:RC_F04465_AT SEC3-like 1 (S. cerevisiae) hs
HU35KSUBB:RC_AA164676_AT SEC3-like 1 (S. cerevisiae) hs
U133_X3P:HS.306648.0.S1_3P_S_AT SEC3-like 1 (S. cerevisiae) hs
HG-U133A_2:222127_S_AT SEC3-like 1 (S. cerevisiae) hs
HG-U133_PLUS_2:222127_S_AT SEC3-like 1 (S. cerevisiae) hs
HG-U133A:222127_S_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95B:44803_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95B:56310_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95C:60944_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95E:80772_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95E:91091_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95B:41961_I_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95B:41962_R_AT SEC3-like 1 (S. cerevisiae) hs
HG-U95E:71794_R_AT SEC3-like 1 (S. cerevisiae) hs
HU35KSUBA:AA504736_AT SEC3-like 1 (S. cerevisiae) hs
MG-U74BV2:109803_AT SEC3-like 1 (S. cerevisiae) mm
MG-U74BV2:108473_AT SEC3-like 1 (S. cerevisiae) mm
MG-U74CV2:130726_AT SEC3-like 1 (S. cerevisiae) mm
MOE430A:1451142_AT SEC3-like 1 (S. cerevisiae) mm
MOUSE430A_2:1451142_AT SEC3-like 1 (S. cerevisiae) mm
MOUSE430_2:1451142_AT SEC3-like 1 (S. cerevisiae) mm
MOE430B:1446105_AT SEC3-like 1 (S. cerevisiae) mm
MOUSE430_2:1446105_AT SEC3-like 1 (S. cerevisiae) mm
MU19KSUBC:TC37612_AT SEC3-like 1 (S. cerevisiae) mm
RAE230A:1375749_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAT230_2:1375749_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAE230B:1383387_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAT230_2:1383387_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAE230B:1393016_S_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAT230_2:1393016_S_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAE230B:1393905_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RAT230_2:1393905_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RG-U34B:RC_AI044665_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
RG-U34C:RC_AI145446_AT SEC3-like 1 (S. cerevisiae) (predicted) rn
YEAST_2:1776988_AT Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion Sc
Protein Similarities
Method ID Description E-Value
blast AAB54035 Hypothetical protein F52E4.7 [Caenorhabditis elegans] ref|NP_508530.1| F52E4.7 [Caenorhabditis elegans] sp|Q20678|SEC3_CAEEL Probable exocyst complex component Sec3 0.0
blast CAE68376 Hypothetical protein CBG14132 [Caenorhabditis briggsae] 0.0
blast AAH54625 Unknown (protein for MGC:64145) [Danio rerio] ref|NP_955891.1| SEC3-like 1 [Danio rerio] 1.0E-130
Sequence
Target Sequence 
>C. ELEGANS:180473_S_AT
gcatatttcaatctttgccgggcggtttgtgacgggatacaaaaagctgccgccaatcaa
tacagcaagtctccgtcgtctgtagtcaagtttgaaaattaccacgagctctatttgact
ttgtcggagctaaaaatctcatgtttggatcaacaacgaaaagatgcgaaagcgctcaag
gaagaacatattgatgcgtacgtaaaagagtttatgggtcgtccactcaaagagattcag
acgttcttcgacaatgtcaacaactttatcgaaacgcgtggaattcgtcctgatgaaatc
tcgtatcaacaacaattcagtcgcattgagcttaaaaaggtaatttcacaacatccggga
aaagaagtgaaaaaaggactcgagcaactgtacaagaaaattgagaagaacctggttgcc
aattcatcacttttgcaagttgtatggagagatatgcaagagcaatttgtcaagcaaatt
gcggaatacaacaaactgattctcacttgttacccaggctcgaagattgagctcgaagtt
tccactgacact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATATTTCAATCTTTGCCGGGCGG 124 301 1969 Antisense
TGCCGGGCGGTTTGTGACGGGATAC 389 579 1984 Antisense
CAAAAAGCTGCCGCCAATCAATACA 168 191 2008 Antisense
AATCAATACAGCAAGTCTCCGTCGT 476 171 2023 Antisense
CTCCGTCGTCTGTAGTCAAGTTTGA 252 235 2039 Antisense
GTTTATGGGTCGTCCACTCAAAGAG 596 449 2178 Antisense
GAGATTCAGACGTTCTTCGACAATG 287 397 2200 Antisense
GAAACGCGTGGAATTCGTCCTGATG 47 359 2239 Antisense
GAACCTGGTTGCCAATTCATCACTT 135 347 2376 Antisense
AACTGATTCTCACTTGTTACCCAGG 312 141 2462 Antisense
TGAGCTCGAAGTTTCCACTGACACT 670 567 2496 Antisense
Sequence Source Affymetrix Proprietary Database