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[180469_s_at]

Description M03F4.8 /REP_DB=WormBase Gene ID /WP=CE12370 /TR=Q23018 /GB=AAB04579.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 39      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 90      6grp_total > 18      4grp_total > 11      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 207.7
(-1.7)
P 		174.6
(-2.3)
P 		139.5
(-3)
P 		456.9
(3)
P 		317
B 206.1
(-1.9)
P 		175
(-2.7)
P 		161.5
(-3)
P 		403.3
(3)
P 		242
C 192.6
(-2.5)
P 		249.8
(-0.3)
P 		180.1
(-3)
P 		335.6
(3)
P 		156
avr 202.1
(-1.9) 		199.8
(-2) 		160.4
(-3) 		398.6
(3) 		238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 229
(3)
P 		135.1
(-2.6)
P 		153.3
(-1.5)
P 		128.1
(-3)
P 		159.6
(-1.1)
P 		171.1
(-0.4)
P 		101
B 156.8
(-0.2)
P 		165.5
(0.5)
P 		123
(-3)
P 		195.9
(3)
P 		160
(0)
P 		148.9
(-0.9)
P 		73
C 179
(3)
P 		103.3
(-3)
P 		156.5
(1.2)
P 		118.8
(-1.8)
P 		149.5
(0.7)
P 		157.1
(1.3)
P 		76
avr 188.3
(3) 		134.6
(-3) 		144.3
(-1.9) 		147.6
(-1.5) 		156.4
(-0.6) 		159
(-0.3) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 75.6
(3)
P 		37.9
(-2.8)
P 		36.5
(-3)
P 		66.9
(1.7)
P 		39
B 65.7
(0.5)
P 		43.2
(-2.3)
P 		37.4
(-3)
P 		85.9
(3)
P 		49
C 63.7
(-0.2)
P 		71.8
(3)
P 		62.8
(-0.6)
P 		56.6
(-3)
P 		15
avr 68.3
(2.6) 		51
(-1.7) 		45.6
(-3) 		69.8
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 42.2
(0.7)
P 		48.1
(3)
P 		33.4
(-2.7)
P 		32.5
(-3)
P 		48.1
(3)
P 		41.7
(0.5)
P 		16
B 54.6
(1.2)
P 		55.1
(1.3)
P 		29.9
(-3)
P 		51.9
(0.8)
P 		65
(3)
P 		55.5
(1.4)
P 		35
C 66.3
(3)
P 		31.5
(-1.9)
P 		39.8
(-0.8)
P 		23.9
(-3)
P 		48.4
(0.5)
P 		52.6
(1.1)
P 		42
avr 54.4
(3) 		44.9
(0.2) 		34.4
(-3) 		36.1
(-2.5) 		53.8
(2.8) 		49.9
(1.7) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180469_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16539
Sequence Type Exemplar sequence
Representative Public ID M03F4.8 NCBI
Target Description M03F4.8 /REP_DB=WormBase Gene ID /WP=CE12370 /TR=Q23018 /GB=AAB04579.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076448(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076448 NCBI Caenorhabditis elegans ZC8.6 (ZC8.6) mRNA, complete cds. 11/11 A 
GENEFINDER00000002224 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4972883:4979561:-1 11/11 None
ZC8.6 ENSEMBL cdna:known chromosome:CEL140:X:4972568:4997565:-1 gene:ZC8.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002213 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4972883-4978375(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC8.6
Entrez Gene 180770 Entrez gene
SwissProt Q23083 EMBL-EBI
WormBase CE38988 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508849 ZC8.6 [Caenorhabditis elegans] 0.0
blast AAM69079 Hypothetical protein ZC8.6 [Caenorhabditis elegans] 0.0
blast CAE68673 Hypothetical protein CBG14577 [Caenorhabditis briggsae] 0.0
blast AAM69079 Hypothetical protein ZC8.6 [Caenorhabditis elegans] 1.0E-140
blast NP_508849 ZC8.6 [Caenorhabditis elegans] 1.0E-137
blast CAE68673 Hypothetical protein CBG14577 [Caenorhabditis briggsae] 1.0E-121
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 3.0E-18
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 5.4E-29
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 4.0E-49
Sequence
Target Sequence 
>C. ELEGANS:180469_S_AT
tcaattccgacagttgacgtggccgccatcgacaatggattggcgttcccgttcaaacat
cctgacgagtggagagcgtatccatttggttgggcacttctcccacaggctcagattccc
ttttccgatgatattgttgacctgcttctgccaaagctggatgatactaattgggtgcgc
gagctatgcggggatcttcgacgtgttttcaagaatgacaaaggttttgacaaaaagatc
ttcgaaaagcaaatgtctgtgatgcgtggacaaattttcaatttgcgagaagcattgatg
aagaaaaagagcccttaccagctcattcagatgccacctcaattgatggttgaagtcaag
caaaagaagaagaagtcaagacgatcacgtaacgctgattacgaagaggcgccgaattat
gattcggacgcttcaaccgtggtaaacaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAATTCCGACAGTTGACGTGGCCG 582 629 484 Antisense
CGACAATGGATTGGCGTTCCCGTTC 436 251 513 Antisense
TTCCCGTTCAAACATCCTGACGAGT 506 697 529 Antisense
CAGATTCCCTTTTCCGATGATATTG 390 205 595 Antisense
GATGATATTGTTGACCTGCTTCTGC 91 411 610 Antisense
GACCTGCTTCTGCCAAAGCTGGATG 422 381 622 Antisense
TGATACTAATTGGGTGCGCGAGCTA 451 565 645 Antisense
CTATGCGGGGATCTTCGACGTGTTT 136 219 667 Antisense
AAAAGAGCCCTTACCAGCTCATTCA 176 133 788 Antisense
CTCATTCAGATGCCACCTCAATTGA 4 231 805 Antisense
GGACGCTTCAACCGTGGTAAACACA 584 521 909 Antisense
Sequence Source Affymetrix Proprietary Database