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[180468_at]

Description C18B2.6 /REP_DB=WormBase Gene ID /WP=CE04045 /TR=Q18077 /GB=AAK39175.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 28      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 41.5
(-2.3)
A 		33.8
(-3)
A 		44.7
(-2)
A 		102
(3)
A 		68
B 89.6
(2.8)
A 		92.2
(3)
A 		29.5
(-2.1)
A 		19.1
(-3)
A 		73
C 43.8
(-0.9)
A 		60.2
(0.6)
A 		21.8
(-3)
A 		85.7
(3)
A 		64
avr 58.3
(1.3) 		62.1
(1.9) 		32
(-3) 		68.9
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 29.7
(-2.2)
A 		28.4
(-2.4)
A 		59.4
(3)
A 		29.6
(-2.2)
A 		50.8
(1.5)
A 		25
(-3)
A 		34
B 61
(3)
A 		51.2
(2)
A 		30.9
(-0.2)
A 		4.5
(-3)
A 		24.2
(-0.9)
A 		37.1
(0.5)
A 		57
C 41.4
(-0.3)
A 		83.6
(3)
A 		7.2
(-3)
A 		37.4
(-0.6)
A 		28.1
(-1.4)
A 		23
(-1.8)
A 		76
avr 44
(1) 		54.4
(3) 		32.5
(-1.3) 		23.8
(-3) 		34.4
(-0.9) 		28.4
(-2.1) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 15.1
(3)
A 		7.3
(-3)
A 		11.7
(0.4)
A 		14.9
(2.8)
A 		8
B 28.6
(3)
A 		22.8
(1.6)
A 		6.8
(-2.3)
A 		4.1
(-3)
A 		25
C 14.5
(1.3)
A 		17.3
(3)
A 		7.6
(-3)
A 		14.5
(1.3)
A 		10
avr 19.4
(3) 		15.8
(1) 		8.7
(-3) 		11.2
(-1.6) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 5.5
(-3)
A 		10.1
(-0.2)
A 		12.9
(1.5)
A 		7.5
(-1.8)
A 		15.3
(3)
A 		6.1
(-2.6)
A 		10
B 21.2
(3)
A 		17
(1.7)
A 		7.5
(-1.1)
A 		1.2
(-3)
A 		9.8
(-0.4)
A 		13.8
(0.8)
A 		20
C 15.3
(0.4)
A 		25.5
(3)
A 		1.8
(-3)
A 		7.5
(-1.6)
A 		9.1
(-1.2)
A 		7.7
(-1.5)
A 		24
avr 14
(1.3) 		17.5
(3) 		7.4
(-2) 		5.4
(-3) 		11.4
(-0) 		9.2
(-1.1) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180468_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18089
Sequence Type Exemplar sequence
Representative Public ID C18B2.6 NCBI
Target Description C18B2.6 /REP_DB=WormBase Gene ID /WP=CE04045 /TR=Q18077 /GB=AAK39175.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076221(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076221 NCBI Caenorhabditis elegans C18B2.6 (C18B2.6) mRNA, complete cds. 11/11 None
GENEFINDER00000016058 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3611708:3617365:-1 11/11 None
SNAP00000016050 ENSEMBL cdna:SNAP chromosome:CEL140:X:3613877:3616205:-1 9/11 None
C18B2.6 ENSEMBL cdna:known chromosome:CEL140:X:3613384:3617365:-1 gene:C18B2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3613384-3617366(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C18B2.6
Entrez Gene 182767 Entrez gene
SwissProt Q18077 EMBL-EBI
WormBase CE04045 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39175 Hypothetical protein C18B2.6 [Caenorhabditis elegans] ref|NP_508622.1| C18B2.6 [Caenorhabditis elegans] 0.0
blast CAE68454 Hypothetical protein CBG14244 [Caenorhabditis briggsae] 0.0
blast NP_071563 amiloride-sensitive cation channel 5, intestinal [Rattus norvegicus] emb|CAB54072.1| amiloride-sensitive Na+ channel [Rattus norvegicus] 3.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 4.5E-21
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 4.5E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508622.1 1 123-145
C18B2.6 1 123-145
GENEFINDER00000016058 1 123-145
SNAP00000016050 2 58-80,437-459
Sequence
Target Sequence 
>C. ELEGANS:180468_AT
aacacaattgtaccactgcttcttcacaaaactgttccccgaggacagcaacctctcaaa
agccattgtggatgcctgtaagaaggagtgtaaagcaccgtgtcatgcctggaattacaa
taagcaagtcagctactcgagcattccaagtgaagccagcaaaaagttattaccaagaga
ggagtgggagaaaatgaaacgaaaaattatacttgacatatactattccgaattggacta
cacaattattaagcacgtcatagcgatgccactctcaagccttattgctcaaattggagg
tcaattctctctgttcgccggtggatccctcatttcactgtgtcaaattgtcatctactc
tgtccgatacgttatgcacaagttctgcgggttgaaaagccaacggcggaggtgtcgaga
gacacgagaagcccacgaggctcgtcaacgacgcgacatgcgacgacgtcgcagaaatag
tacgtcacacagtcggtcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACACAATTGTACCACTGCTTCTTC 111 135 1221 Antisense
CTGCTTCTTCACAAAACTGTTCCCC 541 237 1236 Antisense
AAAGCACCGTGTCATGCCTGGAATT 374 123 1312 Antisense
GCAAGTCAGCTACTCGAGCATTCCA 346 321 1344 Antisense
TAAGCACGTCATAGCGATGCCACTC 504 637 1470 Antisense
CCACTCTCAAGCCTTATTGCTCAAA 346 275 1489 Antisense
TTGGAGGTCAATTCTCTCTGTTCGC 296 709 1514 Antisense
GTCAAATTGTCATCTACTCTGTCCG 273 465 1562 Antisense
CTACTCTGTCCGATACGTTATGCAC 144 227 1575 Antisense
TCAACGACGCGACATGCGACGACGT 696 629 1665 Antisense
TAGTACGTCACACAGTCGGTCAACA 514 651 1698 Antisense
Sequence Source Affymetrix Proprietary Database