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[180444_s_at]

Description ZC477.5 /REP_DB=WormBase Gene ID /WP=CE28164 /TR=Q23342 /GB=AAK19011.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 94      6grp_total > 76      4grp_total > 22      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 369
1 2 3 4 range
A 429.4
(1.9)
P 		287.9
(-0.9)
P 		183.9
(-3)
P 		484.5
(3)
P 		301
B 474.6
(1.3)
P 		351.2
(-1.5)
P 		282.9
(-3)
P 		553
(3)
P 		270
C 460.1
(3)
P 		287.4
(-2.5)
P 		273.2
(-3)
P 		453.8
(2.8)
P 		187
avr 454.7
(2) 		308.8
(-1.5) 		246.7
(-3) 		497.1
(3) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 850
1 2 3 4 5 6 range
A 275.7
(-3)
P 		319.2
(-2.5)
P 		434.1
(-1.1)
P 		388.9
(-1.6)
P 		537.9
(0.2)
P 		771.2
(3)
P 		496
B 301.5
(-3)
P 		404.4
(-1.9)
P 		465.6
(-1.3)
P 		489.6
(-1)
P 		513.5
(-0.8)
P 		875.1
(3)
P 		574
C 304.5
(-3)
P 		339
(-2.7)
P 		352.5
(-2.6)
P 		488.5
(-1.7)
P 		499.4
(-1.6)
P 		1125.9
(3)
P 		821
avr 293.9
(-3) 		354.2
(-2.4) 		417.4
(-1.8) 		455.7
(-1.5) 		516.9
(-0.9) 		924.1
(3) 		630
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 156.4
(3)
P 		62.5
(-2.2)
P 		48.1
(-3)
P 		70.9
(-1.7)
P 		108
B 151.3
(3)
P 		86.7
(-1.5)
P 		65.5
(-3)
P 		117.9
(0.7)
P 		86
C 152.3
(3)
P 		82.6
(-2.5)
P 		95.2
(-1.5)
P 		76.5
(-3)
P 		76
avr 153.3
(3) 		77.3
(-2.4) 		69.6
(-3) 		88.4
(-1.7) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 50.8
(-3)
P 		113.5
(-0.3)
P 		94.5
(-1.1)
P 		98.6
(-0.9)
P 		162.3
(1.9)
P 		187.8
(3)
P 		137
B 104.9
(-3)
P 		134.6
(-2.2)
P 		113.1
(-2.8)
P 		129.7
(-2.3)
P 		208.6
(-0.2)
P 		326.3
(3)
P 		221
C 112.9
(-2.5)
P 		103.4
(-2.7)
P 		89.7
(-3)
P 		98.2
(-2.8)
P 		161.6
(-1.5)
P 		376.9
(3)
P 		287
avr 89.5
(-3) 		117.2
(-2.2) 		99.1
(-2.7) 		108.8
(-2.4) 		177.5
(-0.5) 		297
(3) 		208
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180444_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10231
Sequence Type Exemplar sequence
Representative Public ID ZC477.5 NCBI
Target Description ZC477.5 /REP_DB=WormBase Gene ID /WP=CE28164 /TR=Q23342 /GB=AAK19011.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068706(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068706 NCBI Caenorhabditis elegans ZC477.5 (ZC477.5) mRNA, complete cds. 11/11 None
SNAP00000039646 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7101447:7103513:-1 11/11 None
GENEFINDER00000039663 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7101447:7103072:-1 11/11 None
ZC477.5 ENSEMBL cdna:known chromosome:CEL140:IV:7101420:7103245:-1 gene:ZC477.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7099368-7103078(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC477.5
Entrez Gene 177483 Entrez gene
SwissProt Q23342 EMBL-EBI
WormBase CE28164 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-173
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-169
blast AAK19011 Hypothetical protein ZC477.5 [Caenorhabditis elegans] ref|NP_501107.1| ZC477.5 [Caenorhabditis elegans] 1.0E-149
blast T27604 hypothetical protein ZC477.5 - Caenorhabditis elegans 1.0E-147
blast T27604 hypothetical protein ZC477.5 - Caenorhabditis elegans 1.0E-144
blast CAE61801 Hypothetical protein CBG05767 [Caenorhabditis briggsae] 3.0E-62
Sequence
Target Sequence 
>C. ELEGANS:180444_S_AT
aaggaacgtcgtgtgctatccgaacctgaaaagaaaaaatcaaaaacgatggcccttctg
aacgatcttcttgcgtacggacgccaaaattgtcctggtgtcattgaggagattttgaag
aaaccagtgcatgttcccaacgaccactctacacaacaaccacagccacctgagcaacca
attcgtcgagttgatattgctgatgaagagcacattgctgtttacgttggtgatggtgat
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGAACGTCGTGTGCTATCCGAAC 25 161 667 Antisense
GTGTGCTATCCGAACCTGAAAAGAA 710 487 677 Antisense
AAAAACGATGGCCCTTCTGAACGAT 67 129 708 Antisense
CCCTTCTGAACGATCTTCTTGCGTA 494 259 719 Antisense
GATCTTCTTGCGTACGGACGCCAAA 558 419 730 Antisense
CGGACGCCAAAATTGTCCTGGTGTC 443 241 744 Antisense
AAGAAACCAGTGCATGTTCCCAACG 634 151 784 Antisense
CACCTGAGCAACCAATTCGTCGAGT 519 201 833 Antisense
ATTCGTCGAGTTGATATTGCTGATG 307 9 847 Antisense
AGAGCACATTGCTGTTTACGTTGGT 72 69 873 Antisense
GCTGTTTACGTTGGTGATGGTGATG 204 295 883 Antisense
Sequence Source Affymetrix Proprietary Database