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[180412_at]

Description R05H10.1 /REP_DB=WormBase Gene ID /WP=CE18104 /TR=O62324 /GB=CAB05578.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 30      4grp_highest_8_8 > 23      4grp_high_8_8 > 14      6grp_highest_nonorm > 32      6grp_total > 44      4grp_total > 02      6grp_high1_sub > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6235
1 2 3 4 range
A 539.4
(-3)
P 		844.8
(-2.7)
P 		651.5
(-2.9)
P 		6774.4
(3)
P 		6235
B 554.7
(-3)
P 		733.9
(-2.8)
P 		647.9
(-2.9)
P 		5731.2
(3)
P 		5177
C 675.1
(-3)
P 		737.1
(-2.9)
P 		638.2
(-3)
P 		6201.4
(3)
P 		5563
avr 589.7
(-3) 		771.9
(-2.8) 		645.9
(-2.9) 		6235.7
(3) 		5646

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 980
1 2 3 4 5 6 range
A 1187
(1)
P 		1188.2
(1)
P 		1479.8
(3)
P 		1086.7
(0.3)
P 		597.8
(-3)
P 		1004
(-0.2)
P 		882
B 1059.7
(-0.4)
P 		1165
(0.3)
P 		1577.7
(3)
P 		1043.6
(-0.5)
P 		665.6
(-3)
P 		931.8
(-1.2)
P 		912
C 1148.1
(1.2)
P 		1122.5
(1)
P 		1369.9
(3)
P 		1084
(0.7)
P 		611.6
(-3)
P 		1035.9
(0.4)
P 		758
avr 1131.6
(0.6) 		1158.6
(0.8) 		1475.8
(3) 		1071.4
(0.1) 		625
(-3) 		990.6
(-0.4) 		851
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1071
1 2 3 4 range
A 196.4
(-2.8)
P 		183.4
(-2.9)
P 		170.3
(-3)
P 		991.6
(3)
P 		821
B 176.9
(-2.8)
P 		181.3
(-2.8)
P 		150.1
(-3)
P 		1221.3
(3)
P 		1071
C 223.5
(-2.9)
P 		211.9
(-3)
P 		222.4
(-2.9)
P 		1045.6
(3)
P 		834
avr 198.9
(-2.9) 		192.2
(-2.9) 		180.9
(-3) 		1086.2
(3) 		905

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 218.8
(-2)
P 		422.6
(3)
P 		322.3
(0.5)
P 		275.6
(-0.6)
P 		180.4
(-3)
P 		244.5
(-1.4)
P 		242
B 368.7
(2)
P 		387.8
(3)
P 		383.1
(2.8)
P 		276.4
(-2.7)
P 		270.4
(-3)
P 		347.5
(0.9)
P 		117
C 425.6
(3)
P 		342.5
(0.8)
P 		348.7
(1)
P 		218
(-2.5)
P 		197.9
(-3)
P 		346.8
(0.9)
P 		228
avr 337.7
(1.3) 		384.3
(3) 		351.4
(1.8) 		256.7
(-1.6) 		216.2
(-3) 		312.9
(0.5) 		168
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180412_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3988
Sequence Type Exemplar sequence
Representative Public ID R05H10.1 NCBI
Target Description R05H10.1 /REP_DB=WormBase Gene ID /WP=CE18104 /TR=O62324 /GB=CAB05578.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064675 NCBI Caenorhabditis elegans R05H10.1 (R05H10.1) mRNA, complete cds. 11/11 None
SNAP00000021680 ENSEMBL cdna:SNAP chromosome:CEL140:II:14851609:14856839:1 11/11 None
GENEFINDER00000021685 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14852329:14853391:1 11/11 None
R05H10.1 ENSEMBL cdna:known chromosome:CEL140:II:14852320:14853452:1 gene:R05H10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14852325-14853388(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05H10.1
Entrez Gene 175144 Entrez gene
SwissProt O62324 EMBL-EBI
WormBase CE18104 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05578 Hypothetical protein R05H10.1 [Caenorhabditis elegans] ref|NP_497076.1| R05H10.1 [Caenorhabditis elegans] 1.0E-142
blast CAB05578 Hypothetical protein R05H10.1 [Caenorhabditis elegans] ref|NP_497076.1| R05H10.1 [Caenorhabditis elegans] 1.0E-120
blast CAE17866 Hypothetical protein R05H10.7 [Caenorhabditis elegans] ref|NP_001022271.1| R05H10.7 [Caenorhabditis elegans] 1.0E-102
blast CAE65891 Hypothetical protein CBG11056 [Caenorhabditis briggsae] 1.0E-84
blast CAE65892 Hypothetical protein CBG11057 [Caenorhabditis briggsae] 3.0E-68
blast CAE65892 Hypothetical protein CBG11057 [Caenorhabditis briggsae] 4.0E-52
Sequence
Target Sequence 
>C. ELEGANS:180412_AT
ccagcagtctgaaacttccgatctccacagtagtaacaactgaagacggtgagaacattg
ttgcggtgttattgaattcaatgtggagtcgagaggaggacgaggagaggatgaagcatg
gaaatggaaaaggcgaccatgatactagtggatactcggaggctctgcagagatttatga
ccatcgttcaaaaatgccatgacgagttctggaacctcgcaccatcagacgtgaacctag
tcgtctaccgcgagatctcaagcgtcggaaaaccatggcaacgccaggggatcgccacaa
aaatgctctccaggaacatgtcggctgctaggcttcacaacgtagatggaatcgtatctg
ccacgtcatcattcgccaatcagactctgttggcaaaaaatgggttccagtgtttgaagg
aatttccatattcgggaattgtgagcagtaacggggacaaactggtggagactgatgatg
gatcccatggaatgcggatcaacttcaagagaatcgaaaaatcgggaaatggaccctgtg
aaatggaacccccaaaatctgctact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAGCAGTCTGAAACTTCCGATCTC 669 269 167 Antisense
TTCCGATCTCCACAGTAGTAACAAC 492 697 182 Antisense
GGATACTCGGAGGCTCTGCAGAGAT 281 511 316 Antisense
TGACGAGTTCTGGAACCTCGCACCA 181 571 366 Antisense
CTCGCACCATCAGACGTGAACCTAG 22 233 382 Antisense
CTACCGCGAGATCTCAAGCGTCGGA 545 225 411 Antisense
GATCGCCACAAAAATGCTCTCCAGG 269 417 456 Antisense
CTCTCCAGGAACATGTCGGCTGCTA 167 231 472 Antisense
GCTGCTAGGCTTCACAACGTAGATG 203 297 490 Antisense
GATGGAATCGTATCTGCCACGTCAT 529 405 511 Antisense
AATGGAACCCCCAAAATCTGCTACT 199 163 708 Antisense
Sequence Source Affymetrix Proprietary Database