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[180407_at]

Description F36D4.5 /REP_DB=WormBase Gene ID /WP=CE28391 /TR=Q20103 /GB=AAA93489.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 86      6grp_total > 69      4grp_total > 39      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 251
1 2 3 4 range
A 922.3
(3)
P 		769.2
(-3)
P 		864.3
(0.7)
P 		779.3
(-2.6)
P 		153
B 917
(3)
P 		706.6
(-2.1)
P 		671.3
(-3)
P 		805
(0.3)
P 		246
C 850.3
(3)
P 		772.1
(-1.3)
P 		739.9
(-3)
P 		820.6
(1.4)
P 		110
avr 896.5
(3) 		749.3
(-3) 		758.5
(-2.6) 		801.6
(-0.9) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 497
1 2 3 4 5 6 range
A 839.6
(-0.5)
P 		792
(-1.1)
P 		636.7
(-3)
P 		743.3
(-1.7)
P 		1100.4
(2.6)
P 		1133.2
(3)
P 		497
B 963.5
(1.1)
P 		1037.2
(2.2)
P 		681.4
(-3)
P 		755.4
(-1.9)
P 		816.2
(-1)
P 		1091.6
(3)
P 		410
C 825.6
(-1.8)
P 		987.6
(1)
P 		753.6
(-3)
P 		1027
(1.7)
P 		877
(-0.9)
P 		1104
(3)
P 		350
avr 876.2
(-0.3) 		938.9
(0.6) 		690.6
(-3) 		841.9
(-0.8) 		931.2
(0.4) 		1109.6
(3) 		419
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 335.8
(3)
P 		167
(-1.6)
P 		225.9
(0)
P 		114.1
(-3)
P 		222
B 292.4
(3)
P 		174.5
(-2.2)
P 		155.6
(-3)
P 		171.6
(-2.3)
P 		137
C 281.5
(3)
P 		222
(0.5)
P 		257.9
(2)
P 		138.4
(-3)
P 		143
avr 303.2
(3) 		187.8
(-1.3) 		213.1
(-0.3) 		141.4
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 154.8
(-2.5)
P 		281.7
(1.4)
P 		138.7
(-3)
P 		188.5
(-1.5)
P 		332
(3)
P 		275.9
(1.3)
P 		193
B 335.3
(1.2)
P 		345.2
(1.5)
P 		165.5
(-3)
P 		200.1
(-2.1)
P 		331.6
(1.1)
P 		407.1
(3)
P 		242
C 306
(0.9)
P 		301.3
(0.7)
P 		191.8
(-3)
P 		206.5
(-2.5)
P 		283.8
(0.1)
P 		369.6
(3)
P 		178
avr 265.4
(0.2) 		309.4
(1.7) 		165.3
(-3) 		198.4
(-1.9) 		315.8
(1.9) 		350.9
(3) 		186
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11611
Sequence Type Exemplar sequence
Representative Public ID F36D4.5 NCBI
Target Description F36D4.5 /REP_DB=WormBase Gene ID /WP=CE28391 /TR=Q20103 /GB=AAA93489.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171507(10), NM_171506(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171507 NCBI Caenorhabditis elegans F36D4.5b (F36D4.5) mRNA, complete cds. 10/11 None
NM_171506 NCBI Caenorhabditis elegans F36D4.5a (F36D4.5) mRNA, complete cds. 10/11 None
SNAP00000027393 ENSEMBL cdna:SNAP chromosome:CEL140:V:9422381:9424034:-1 10/11 None
F36D4.5b.1 ENSEMBL cdna:known chromosome:CEL140:V:9421879:9424086:-1 gene:F36D4.5 10/11 A 
F36D4.5b.4 ENSEMBL cdna:known chromosome:CEL140:V:9421879:9426475:-1 gene:F36D4.5 10/11 A 
F36D4.5b.3 ENSEMBL cdna:known chromosome:CEL140:V:9421879:9424088:-1 gene:F36D4.5 10/11 A 
F36D4.5b.2 ENSEMBL cdna:known chromosome:CEL140:V:9421879:9428343:-1 gene:F36D4.5 10/11 A 
F36D4.5a ENSEMBL cdna:known chromosome:CEL140:V:9422975:9428300:-1 gene:F36D4.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9422380-9424086(-) 99.57 99.57
Public Domain and Genome References
Ensembl F36D4.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA93489 Hypothetical protein F36D4.5a [Caenorhabditis elegans] ref|NP_741591.1| F36D4.5a [Caenorhabditis elegans] 1.0E-178
blast AAA93489 Hypothetical protein F36D4.5a [Caenorhabditis elegans] ref|NP_741591.1| F36D4.5a [Caenorhabditis elegans] 1.0E-166
blast AAM29689 Hypothetical protein F36D4.5b [Caenorhabditis elegans] ref|NP_741592.1| F36D4.5b [Caenorhabditis elegans] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR008580 EMBL-EBI Eukaryotic protein of unknown function DUF862 3.1E-84
Pfam IPR008580 EMBL-EBI Eukaryotic protein of unknown function DUF862 3.1E-84
Sequence
Target Sequence 
>C. ELEGANS:180407_AT
gttgcaggcctctgtcgatgagaagaagagaggatcagtagattcagcagaagaagcaac
tgagaaactagtggttcgatcgctgaatgactcccgcactacaattcttgataatcgaac
cgcaaatggagccataataatgtcagcttcttcatcaaattctgatcgtatctgcatgtc
cccatcatcttcttcttctgcttcatcctgtgatactcttgactatgacgatcttattgt
tcaaacaccgtctacgttttcaagtgaaaagaaatctcgtagcaactcaccgccaatttt
ccgcatctggaatactatcaaagcaacaataaacggaactcaacagacagctcctaccgg
agccgcaactgtgattccagcctcttcggcctcaaatattggaaaaacgaacagtacgcc
tggcacaacaagcaatggtcttgcaaaaccaacatgttctgaattccgaaaaaaccaaaa
agaaccaaataatcaatttttgccaaaaaaacgacaaccctgtttcttacttgttaaaga
tgaactatttggaaatattacaggttttatttatttcagattcctcgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGCAGGCCTCTGTCGATGAGAAG 517 439 549 Antisense
AGTGGTTCGATCGCTGAATGACTCC 89 59 618 Antisense
ACTCCCGCACTACAATTCTTGATAA 560 99 638 Antisense
AATTCTGATCGTATCTGCATGTCCC 535 177 706 Antisense
GCTTCATCCTGTGATACTCTTGACT 568 305 748 Antisense
AAGAAATCTCGTAGCAACTCACCGC 65 153 817 Antisense
ACGGAACTCAACAGACAGCTCCTAC 378 95 881 Antisense
TCCTACCGGAGCCGCAACTGTGATT 345 591 900 Antisense
AGCCTCTTCGGCCTCAAATATTGGA 68 85 927 Antisense
AGTACGCCTGGCACAACAAGCAATG 433 61 961 Antisense
TTATTTATTTCAGATTCCTCGCCGA 359 679 1115 Antisense
Sequence Source Affymetrix Proprietary Database