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[180402_at]

Description D1037.2 /REP_DB=WormBase Gene ID /WP=CE20621 /TR=Q9TYS4 /GB=AAK21365.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 33      6grp_cgc > 99      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 15.8
(-2.2)
A 		67.6
(3)
A 		7.9
(-3)
A 		21.3
(-1.7)
A 		60
B 28.8
(-3)
A 		65.7
(-0.5)
A 		116
(3)
A 		47.4
(-1.7)
A 		87
C 29.2
(-3)
A 		78.6
(0.8)
A 		40.9
(-2.1)
A 		108.1
(3)
A 		79
avr 24.6
(-3) 		70.6
(3) 		54.9
(1) 		58.9
(1.5) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 33.5
(-1.4)
A 		36.2
(-1.2)
A 		50.6
(0.1)
A 		80.1
(2.7)
A 		83.9
(3)
A 		15.9
(-3)
A 		68
B 33.6
(1.6)
A 		19.8
(-1.2)
A 		11
(-3)
A 		12
(-2.8)
A 		40.6
(3)
A 		28.2
(0.5)
A 		30
C 41.8
(-0.6)
A 		14.5
(-2.4)
A 		4.4
(-3)
A 		10.6
(-2.6)
A 		99.6
(3)
A 		33.4
(-1.2)
A 		95
avr 36.3
(-1.4) 		23.5
(-2.8) 		22
(-3) 		34.2
(-1.6) 		74.7
(3) 		25.8
(-2.6) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 5.8
(-1.2)
A 		14.7
(3)
A 		2.1
(-3)
A 		3.1
(-2.5)
A 		13
B 9.2
(-3)
A 		16.2
(-0.6)
A 		26.9
(3)
A 		10.1
(-2.7)
A 		18
C 9.7
(-3)
A 		22.6
(3)
A 		14.2
(-0.9)
A 		18.2
(1)
A 		13
avr 8.2
(-3) 		17.8
(3) 		14.4
(0.9) 		10.5
(-1.6) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 6.2
(-2.4)
A 		12.9
(-0.5)
A 		11
(-1)
A 		20.3
(1.6)
A 		25.3
(3)
A 		3.9
(-3)
A 		21
B 11.7
(0.9)
A 		6.6
(-1.3)
A 		2.7
(-3)
A 		3.2
(-2.8)
A 		16.5
(3)
A 		10.5
(0.4)
A 		14
C 15.5
(-0.2)
A 		4.4
(-2.4)
A 		1.1
(-3)
A 		2.1
(-2.8)
A 		32.2
(3)
A 		11.2
(-1.1)
A 		31
avr 11.1
(-1.1) 		8
(-2.1) 		4.9
(-3) 		8.5
(-1.9) 		24.7
(3) 		8.5
(-1.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180402_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.70
Sequence Type Exemplar sequence
Representative Public ID D1037.2 NCBI
Target Description D1037.2 /REP_DB=WormBase Gene ID /WP=CE20621 /TR=Q9TYS4 /GB=AAK21365.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058796(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058796 NCBI Caenorhabditis elegans SeMaPhorin related family member (smp-2) (smp-2) mRNA, complete cds. 11/11 None
GENEFINDER00000024414 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3666605:3671663:-1 11/11 None
D1037.2 ENSEMBL cdna:known chromosome:CEL140:I:3667126:3671702:-1 gene:D1037.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024405 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3667125-3671868(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol smp-2
Ensembl D1037.2
Entrez Gene 183905 Entrez gene
SwissProt Q9TYS4 EMBL-EBI
WormBase CE37337 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7275 development inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21365 Semaphorin related protein 2 [Caenorhabditis elegans] ref|NP_491197.2| SeMaPhorin related family member (smp-2) [Caenorhabditis elegans] 0.0
blast CAE66688 Hypothetical protein CBG12028 [Caenorhabditis briggsae] 0.0
blast EAA10953 ENSANGP00000018681 [Anopheles gambiae str. PEST] ref|XP_315755.2| ENSANGP00000018681 [Anopheles gambiae str. PEST] 8.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.1E-7
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.1E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491197.2 1 565-587
D1037.2 1 565-587
GENEFINDER00000024414 1 565-587
Sequence
Target Sequence 
>C. ELEGANS:180402_AT
aagtgtcctgaaagtcccaacagccacgtgtcacttagcttccaactgcgccgaatgcct
agcgcacggagatccacattgtgcatgggctgatggtgtagattgtattgatattcggac
agatcaacgaaaatctgcaagtcaagacagtggaacgtgtgatgttgtcagcttcgagaa
tcataagccagtgatgattgcgcccctgaaaaataaagaatcagccaaaattccagtttg
tctatgtgaaacagaaaaacagaaaaagccctgtgccactgaagtcatccagaaagagat
cgttcttacaggaggatcagaattttggaagtacatattggtgttcgctgtcggtgttct
aacaggttcaatcttccactactgttatttctatttcagcaggcatattggtgacaaggc
atcttcatcatctcgttcgtcatcatcaatgactcgaccaatcactacctcgtcttcagc
acgcctcccaatcgacgctttcaccacgtcatcaattgcagacgaaatgttctcatcgac
tctttcatctgcaaatcgtttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTGTCCTGAAAGTCCCAACAGCC 341 159 1440 Antisense
CAACAGCCACGTGTCACTTAGCTTC 662 187 1457 Antisense
GCCGAATGCCTAGCGCACGGAGATC 16 283 1489 Antisense
GATCCACATTGTGCATGGGCTGATG 211 415 1510 Antisense
GAAAAAGCCCTGTGCCACTGAAGTC 179 355 1701 Antisense
CATATTGGTGTTCGCTGTCGGTGTT 60 215 1773 Antisense
CTAACAGGTTCAATCTTCCACTACT 206 227 1798 Antisense
GGTGACAAGGCATCTTCATCATCTC 706 499 1849 Antisense
TCATCATCTCGTTCGTCATCATCAA 316 621 1864 Antisense
GACGAAATGTTCTCATCGACTCTTT 302 379 1960 Antisense
GACTCTTTCATCTGCAAATCGTTTC 407 373 1977 Antisense
Sequence Source Affymetrix Proprietary Database