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[180401_at]

Description F22F7.7 /REP_DB=WormBase Gene ID /WP=CE27995 /TR=Q9GZE8 /GB=AAF99914.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 31      4grp_high_8_8 > 12      6grp_highest_nonorm > 90      6grp_total > 28      4grp_total > 12      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 218.6
(-3)
P 		274
(-0.5)
P 		228.6
(-2.5)
P 		351.8
(3)
P 		133
B 279.2
(3)
P 		219.4
(-3)
P 		223.8
(-2.6)
P 		268.8
(2)
P 		60
C 242.6
(0.4)
P 		233.5
(-1)
P 		220.7
(-3)
P 		259.5
(3)
P 		39
avr 246.8
(-1.1) 		242.3
(-1.4) 		224.4
(-3) 		293.4
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 211.1
(3)
P 		167
(0.3)
P 		140
(-1.4)
P 		114.3
(-3)
P 		165.3
(0.2)
P 		181.3
(1.2)
P 		97
B 263.3
(3)
P 		151.3
(-0.7)
P 		132.4
(-1.3)
P 		80.1
(-3)
P 		147.3
(-0.8)
P 		156
(-0.5)
P 		183
C 338.8
(3)
P 		171.6
(-1.4)
P 		111.4
(-3)
P 		165.3
(-1.6)
P 		140
(-2.2)
P 		133.6
(-2.4)
P 		227
avr 271.1
(3) 		163.3
(-1.3) 		127.9
(-2.7) 		119.9
(-3) 		150.9
(-1.8) 		157
(-1.5) 		151
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 79.6
(3)
P 		59.5
(-1.3)
P 		59.8
(-1.2)
P 		51.5
(-3)
P 		28
B 89
(3)
P 		54.2
(-2.6)
P 		51.8
(-3)
P 		57.3
(-2.1)
P 		37
C 80.3
(3)
P 		67.1
(0.8)
P 		76.9
(2.4)
P 		43.8
(-3)
P 		37
avr 83
(3) 		60.3
(-1.2) 		62.8
(-0.8) 		50.9
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 38.9
(-1)
P 		59.4
(3)
P 		30.5
(-2.7)
P 		29
(-3)
P 		49.9
(1.1)
P 		44.1
(-0)
P 		30
B 91.6
(3)
P 		50.4
(-0.5)
P 		32.1
(-2.1)
P 		21.2
(-3)
P 		59.8
(0.3)
P 		58.2
(0.2)
P 		70
C 125.6
(3)
P 		52.4
(-1.5)
P 		28.3
(-3)
P 		33.2
(-2.7)
P 		45.3
(-2)
P 		44.7
(-2)
P 		97
avr 85.4
(3) 		54.1
(-0.3) 		30.3
(-2.7) 		27.8
(-3) 		51.7
(-0.5) 		49
(-0.8) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180401_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15397
Sequence Type Exemplar sequence
Representative Public ID F22F7.7 NCBI
Target Description F22F7.7 /REP_DB=WormBase Gene ID /WP=CE27995 /TR=Q9GZE8 /GB=AAF99914.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071177(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071177 NCBI Caenorhabditis elegans F22F7.7 (F22F7.7) mRNA, complete cds. 11/11 None
GENEFINDER00000032288 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2131918:2139659:-1 11/11 None
F22F7.7.1 ENSEMBL cdna:known chromosome:CEL140:V:2131340:2139870:-1 gene:F22F7.7 11/11 None
F22F7.7.2 ENSEMBL cdna:known chromosome:CEL140:V:2131340:2139660:-1 gene:F22F7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2131917-2134136(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22F7.7
Entrez Gene 178693 Entrez gene
SwissProt Q9GZE8 EMBL-EBI
WormBase CE27995 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.14841.1.S1_AT similar to CG10365-PA, isoform A cfa
CANINE_2:CFAAFFX.14841.1.S1_S_AT similar to CG10365-PA, isoform A cfa
DROSOPHILA_2:1637983_S_AT dm
CHICKEN:GGA.4003.1.S1_AT similar to hypothetical protein MGC4504 gga
HG-U133A:219270_AT hypothetical protein MGC4504 hs
HG-U133_PLUS_2:219270_AT hypothetical protein MGC4504 hs
HG-U133A_2:219270_AT hypothetical protein MGC4504 hs
U133_X3P:G13129125_3P_AT hypothetical protein MGC4504 hs
HG-U95E:90592_AT hypothetical protein MGC4504 hs
MG-U74BV2:115844_AT RIKEN cDNA 1810008K03 gene mm
MOE430A:1451382_AT RIKEN cDNA 1810008K03 gene mm
MOUSE430A_2:1451382_AT RIKEN cDNA 1810008K03 gene mm
MOUSE430_2:1451382_AT RIKEN cDNA 1810008K03 gene mm
RAT230_2:1389573_AT similar to RIKEN cDNA 1810008K03 (predicted) rn
RAE230A:1389573_AT similar to RIKEN cDNA 1810008K03 (predicted) rn
RG-U34C:RC_AI170665_AT similar to RIKEN cDNA 1810008K03 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF99914 Hypothetical protein F22F7.7 [Caenorhabditis elegans] ref|NP_503578.1| F22F7.7 [Caenorhabditis elegans] 1.0E-116
blast CAE71033 Hypothetical protein CBG17874 [Caenorhabditis briggsae] 1.0E-97
blast XP_421133 PREDICTED: similar to hypothetical protein MGC4504 [Gallus gallus] 2.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR006840 EMBL-EBI ChaC-like protein 3.1E-110
Pfam IPR006840 EMBL-EBI ChaC-like protein 3.1E-110
Sequence
Target Sequence 
>C. ELEGANS:180401_AT
ccacgtcatcgactagccaatcactctggattttcggctacggaagtctcatctggaacc
ctggattcacgttctccacgtcacgaaaagcctacgctatcggatgggctcgacgaatgt
atcaaggaaacacatatcatcgaggtgacgagaaattgcccggacgcgtcgccaccctta
tcgaggagaccaactcttacacgaacggagtcgtgttccgtgtcgacggaaaatccgcca
tcgccactgctgtcaagtatctcgagcagagagagtgcgacaacggctacgcattccgaa
tggtgcctgttcaaatcagatctgctgctcaccgccgtcctacagtagtcatggcgctga
cctgcgtggccgatcagcagaatgagctgtacctcggccccgatgacctcatcaaaatgg
cacgagaaatcgtgacggcaaaaggatgcgccggaccaaattgcgaatacgtcctcaatc
tggccgaaaatttgcggaaactcttccccaacgacgaggacgaccatctttttcaactcg
agc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACGTCATCGACTAGCCAATCACT 259 275 26 Antisense
GGCTACGGAAGTCTCATCTGGAACC 483 537 61 Antisense
TCTGGAACCCTGGATTCACGTTCTC 707 607 77 Antisense
ACGTTCTCCACGTCACGAAAAGCCT 516 93 94 Antisense
GCCTACGCTATCGGATGGGCTCGAC 158 277 115 Antisense
GGTGACGAGAAATTGCCCGGACGCG 228 497 169 Antisense
TCGCCACTGCTGTCAAGTATCTCGA 261 599 266 Antisense
GCCGTCCTACAGTAGTCATGGCGCT 296 283 359 Antisense
TGACCTGCGTGGCCGATCAGCAGAA 330 569 383 Antisense
ATACGTCCTCAATCTGGCCGAAAAT 632 21 492 Antisense
GACGACCATCTTTTTCAACTCGAGC 462 379 544 Antisense
Sequence Source Affymetrix Proprietary Database