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[180399_at]

Description H25P06.4 /REP_DB=WormBase Gene ID /WP=CE18825 /TR=Q9XU14 /GB=CAB07236.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 52      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 45.2
(-0.4)
A 		80.5
(3)
A 		27.2
(-2.1)
A 		17.3
(-3)
A 		63
B 75.5
(2.9)
A 		48.4
(-3)
A 		76.1
(3)
A 		58.9
(-0.7)
A 		28
C 51.4
(3)
A 		46.7
(1.7)
A 		50.2
(2.7)
A 		29.5
(-3)
A 		22
avr 57.4
(2.7) 		58.5
(3) 		51.2
(1.1) 		35.2
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 38.7
(0.4)
A 		45.4
(1.7)
A 		52.4
(3)
A 		20.7
(-3)
A 		45.9
(1.8)
A 		40.9
(0.8)
A 		32
B 51.7
(0.9)
A 		67.3
(3)
A 		56.7
(1.5)
A 		23.5
(-3)
A 		29
(-2.2)
A 		31
(-2)
A 		44
C 59.7
(1.2)
A 		74.4
(3)
A 		24.5
(-3)
A 		38
(-1.4)
A 		54
(0.5)
A 		43.6
(-0.7)
A 		50
avr 50
(0.9) 		62.4
(3) 		44.5
(-0.1) 		27.4
(-3) 		43
(-0.3) 		38.5
(-1.1) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 16.5
(2.6)
A 		17.5
(3)
A 		7.1
(-1.2)
A 		2.5
(-3)
A 		15
B 24.1
(3)
A 		11.9
(-3)
A 		17.6
(-0.2)
A 		12.6
(-2.7)
A 		12
C 17
(2.8)
A 		13.4
(1)
A 		17.5
(3)
A 		5
(-3)
A 		13
avr 19.2
(3) 		14.3
(0.6) 		14.1
(0.6) 		6.7
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 7.1
(-2)
A 		16.2
(3)
A 		11.4
(0.4)
A 		5.2
(-3)
A 		13.9
(1.7)
A 		10
(-0.4)
A 		11
B 18
(1.4)
A 		22.4
(3)
A 		13.8
(-0.2)
A 		6.2
(-3)
A 		11.8
(-0.9)
A 		11.6
(-1)
A 		16
C 22.1
(2.8)
A 		22.7
(3)
A 		6.2
(-3)
A 		7.6
(-2.5)
A 		17.5
(1.1)
A 		14.6
(0.1)
A 		17
avr 15.7
(1) 		20.4
(3) 		10.5
(-1.2) 		6.3
(-3) 		14.4
(0.4) 		12.1
(-0.5) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180399_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.716
Sequence Type Exemplar sequence
Representative Public ID H25P06.4 NCBI
Target Description H25P06.4 /REP_DB=WormBase Gene ID /WP=CE18825 /TR=Q9XU14 /GB=CAB07236.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060503(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060503 NCBI Caenorhabditis elegans H25P06.4 (H25P06.4) mRNA, complete cds. 11/11 A 
SNAP00000026533 ENSEMBL cdna:SNAP chromosome:CEL140:I:11313944:11314719:-1 10/11 None
GENEFINDER00000026542 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11313976:11318996:-1 11/11 None
H25P06.4 ENSEMBL cdna:known chromosome:CEL140:I:11313944:11317724:-1 gene:H25P06.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11311793-11313243(+) 56.81 56.81
chrI:11313874-11317655(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H25P06.4
Entrez Gene 186784 Entrez gene
SwissProt Q9XU14 EMBL-EBI
WormBase CE18825 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
46983 protein dimerization activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07236 Hypothetical protein H25P06.4 [Caenorhabditis elegans] ref|NP_492904.1| H25P06.4 [Caenorhabditis elegans] 0.0
blast CAB60415 Hypothetical protein Y53H1B.6 [Caenorhabditis elegans] ref|NP_492903.1| Y53H1B.6 [Caenorhabditis elegans] 1.0E-141
blast CAB07236 Hypothetical protein H25P06.4 [Caenorhabditis elegans] ref|NP_492904.1| H25P06.4 [Caenorhabditis elegans] 1.0E-106
blast CAB04898 Hypothetical protein W04A4.4 [Caenorhabditis elegans] ref|NP_493397.2| W04A4.4 [Caenorhabditis elegans] 2.0E-35
blast CAB04898 Hypothetical protein W04A4.4 [Caenorhabditis elegans] ref|NP_493397.2| W04A4.4 [Caenorhabditis elegans] 8.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR008906 EMBL-EBI HAT dimerisation 1.2E-25
Pfam IPR008906 EMBL-EBI HAT dimerisation 1.2E-25
Pfam IPR008906 EMBL-EBI HAT dimerisation 1.2E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026542 1 27-49
Sequence
Target Sequence 
>C. ELEGANS:180399_AT
agagacaccgaaactacctgctctacgaatcaagacaaaattggtttaatcagtgctgtt
gaaaagaattggggctcgaatccggataaaccgaatcaaaaaactcttaaaagatccact
ataagcgacaattcgtgcgagccggtcgcaaaaaaaccggaatttgatttcaaaacattt
atgcaggaattggaacgctatctcgcgtgcgacgatgaagtcacgcctccctatgagttt
tggacagatccgttgaataaatcagcttgtccacgtttggcaaaattcgctgcccaattc
ttcatttgcccaccaggaagtgctgaagttgagcggtttttctccggagctgggcaaatt
ctgtcaaaatataggaagtcgttgagccctgagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGACACCGAAACTACCTGCTCTA 424 69 727 Antisense
GAATTGGGGCTCGAATCCGGATAAA 705 321 792 Antisense
TAAGCGACAATTCGTGCGAGCCGGT 415 637 848 Antisense
GCAGGAATTGGAACGCTATCTCGCG 328 311 909 Antisense
CGTGCGACGATGAAGTCACGCCTCC 653 245 932 Antisense
TCACGCCTCCCTATGAGTTTTGGAC 469 621 947 Antisense
AAATCAGCTTGTCCACGTTTGGCAA 388 117 985 Antisense
AATTCTTCATTTGCCCACCAGGAAG 377 177 1022 Antisense
GAAGTTGAGCGGTTTTTCTCCGGAG 521 331 1051 Antisense
TCTCCGGAGCTGGGCAAATTCTGTC 78 611 1067 Antisense
ATAGGAAGTCGTTGAGCCCTGAGAG 45 21 1097 Antisense
Sequence Source Affymetrix Proprietary Database