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[180395_at]

Description F28C12.6 /REP_DB=WormBase Gene ID /WP=CE09753 /TR=O17845 /GB=CAB07601.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 53      4grp_total > 34      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 5.6
(-3)
A 		14.9
(-1.1)
A 		35.3
(3)
A 		15.5
(-1)
A 		30
B 5.1
(-2.9)
A 		22
(3)
A 		4.9
(-3)
A 		19.7
(2.2)
A 		17
C 15.6
(-0.4)
A 		25.2
(2.6)
A 		7.6
(-3)
A 		26.4
(3)
A 		19
avr 8.8
(-3) 		20.7
(3) 		15.9
(0.6) 		20.5
(2.9) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 15.7
(2)
A 		5.8
(-1.6)
A 		18.6
(3)
A 		7.8
(-0.9)
A 		1.9
(-3)
A 		4.6
(-2)
A 		17
B 5.3
(-1.9)
A 		1.3
(-3)
A 		8.6
(-1)
A 		3.4
(-2.4)
A 		23.7
(3)
A 		10.2
(-0.6)
A 		22
C 6.2
(-2.5)
A 		3.7
(-3)
A 		33
(3)
A 		7.1
(-2.3)
A 		3.7
(-3)
A 		5.2
(-2.7)
A 		29
avr 9.1
(-1) 		3.6
(-3) 		20.1
(3) 		6.1
(-2.1) 		9.8
(-0.7) 		6.7
(-1.9) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2
(-3)
A 		3.2
(-2)
A 		9.2
(3)
A 		2.3
(-2.8)
A 		7
B 1.6
(-2.3)
A 		5.4
(3)
A 		1.1
(-3)
A 		4.2
(1.3)
A 		4
C 5.2
(0.3)
A 		7.2
(3)
A 		2.7
(-3)
A 		4.5
(-0.6)
A 		5
avr 2.9
(-3) 		5.3
(3) 		4.3
(0.5) 		3.7
(-1) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 2.9
(1.1)
A 		2.1
(-0.4)
A 		4
(3)
A 		2
(-0.5)
A 		0.6
(-3)
A 		1.1
(-2.1)
A 		3
B 1.8
(-2.1)
A 		0.4
(-3)
A 		2.1
(-1.9)
A 		0.9
(-2.7)
A 		9.6
(3)
A 		3.8
(-0.8)
A 		9
C 2.3
(-2)
A 		1.1
(-3)
A 		8.4
(3)
A 		1.4
(-2.8)
A 		1.2
(-2.9)
A 		1.7
(-2.5)
A 		7
avr 2.3
(-1.2) 		1.2
(-3) 		4.8
(3) 		1.4
(-2.7) 		3.8
(1.3) 		2.2
(-1.3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180395_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.695
Sequence Type Exemplar sequence
Representative Public ID F28C12.6 NCBI
Target Description F28C12.6 /REP_DB=WormBase Gene ID /WP=CE09753 /TR=O17845 /GB=CAB07601.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060812(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060812 NCBI Caenorhabditis elegans F28C12.6 (F28C12.6) mRNA, complete cds. 11/11 A 
F28C12.6 ENSEMBL cdna:known chromosome:CEL140:I:12699119:12700036:1 gene:F28C12.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017838 8/11 Cross Hyb Matching Probes None
SNAP00000008987 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12699049-12699967(+) 62.24 100.0
Public Domain and Genome References
Gene Title
Ensembl F28C12.6
Entrez Gene 185058 Entrez gene
SwissProt O17845 EMBL-EBI
WormBase CE09753 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07601 Hypothetical protein F28C12.6 [Caenorhabditis elegans] ref|NP_493213.1| F28C12.6 [Caenorhabditis elegans] 1.0E-108
blast CAB55054 Hypothetical protein Y50E8A.15 [Caenorhabditis elegans] ref|NP_506643.1| Y50E8A.15 [Caenorhabditis elegans] 2.0E-33
blast AAF36060 Hypothetical protein Y75D11A.5 [Caenorhabditis elegans] ref|NP_508324.1| Y75D11A.5 [Caenorhabditis elegans] 9.0E-15
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493213.1 1 194-216
F28C12.6 1 194-216
Sequence
Target Sequence 
>C. ELEGANS:180395_AT
aacaatcaagtccaaccaattcggacaccttcaagtcaacaacaagcccggaaaagtggg
gcagcatcggatcaagacaaggagtcagtgcaacgacttcatcaagcaacttcaagagtc
aacttcaatcaacgtacagtcacaatcaacccacacgatgacaccgacgatgccgatcaa
gctgagaagagctccgaaaaagaggtgtacgatgaaatcgctgagatagctgatatcacc
aatagcattcgtgctgctattgaaaagataaggaactacgatcaacaatggaaggatctc
ttcacgagaattccaacggagagagaaggcatggccgactacaagaaacggctaggcgac
tacgatagtttattgcaattcataagaagaaagctccacaaccgacagattatccaacat
tcggtggagatgaagcaagtccaaatcaatcatcgtcgtcaaacacaacggatcatcaaa
ccaaaacatatatttcgaatcatatttctgattattattattgttattatcaataaagct
tttacattattgtctacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAATCAAGTCCAACCAATTCGGA 552 133 106 Antisense
CCAACCAATTCGGACACCTTCAAGT 3 277 117 Antisense
ACCTTCAAGTCAACAACAAGCCCGG 269 87 132 Antisense
AGTGGGGCAGCATCGGATCAAGACA 476 59 160 Antisense
GGAGTCAGTGCAACGACTTCATCAA 387 517 186 Antisense
GACTTCATCAAGCAACTTCAAGAGT 17 371 200 Antisense
AGAGTCAACTTCAATCAACGTACAG 360 69 220 Antisense
CAACGTACAGTCACAATCAACCCAC 51 187 235 Antisense
TCACAATCAACCCACACGATGACAC 306 629 245 Antisense
TAGGCGACTACGATAGTTTATTGCA 515 565 458 Antisense
AAAGCTTTTACATTATTGTCTACTA 140 125 640 Antisense
Sequence Source Affymetrix Proprietary Database