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[180376_s_at]

Description M03A1.3 /REP_DB=WormBase Gene ID /WP=CE07390 /TR=Q21476 /GB=AAA93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 60      4grp_high_8_8 > 54      6grp_highest_nonorm > 25      6grp_total > 61      4grp_total > 49      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8347
1 2 3 4 range
A 46.8
(-3)
P 		7481.1
(3)
P 		7531.8
(3)
P 		93.1
(-3)
P 		7485
B 31.6
(-3)
M 		6584.8
(1.7)
P 		8378.3
(3)
P 		152.9
(-2.9)
P 		8347
C 47.6
(-3)
P 		6587.5
(2.4)
P 		7300.2
(3)
P 		159.2
(-2.9)
P 		7253
avr 42
(-3) 		6884.5
(2.3) 		7736.8
(3) 		135.1
(-2.9) 		7695

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4944
1 2 3 4 5 6 range
A 4317
(0.4)
P 		3739.2
(-0.3)
P 		5475.7
(1.8)
P 		6484.7
(3)
P 		3847.3
(-0.2)
P 		1540.3
(-3)
P 		4944
B 4382.1
(0.8)
P 		4170.1
(0.5)
P 		4492.7
(0.9)
P 		5966.2
(3)
P 		4374.7
(0.8)
P 		1706.6
(-3)
P 		4260
C 4545.2
(2.6)
P 		4179.7
(1.9)
P 		4304.1
(2.1)
P 		4713.2
(3)
P 		4172.8
(1.8)
P 		1894.2
(-3)
P 		2819
avr 4414.8
(1) 		4029.7
(0.5) 		4757.5
(1.6) 		5721.4
(3) 		4131.6
(0.6) 		1713.7
(-3) 		4008
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2534
1 2 3 4 range
A 17
(-3)
P 		1624
(1.9)
P 		1969
(3)
P 		13.6
(-3)
P 		1955
B 10.1
(-3)
M 		1626.3
(2)
P 		1941.4
(3)
P 		32.6
(-2.9)
P 		1931
C 15.7
(-3)
P 		1893.9
(1.5)
P 		2544.3
(3)
P 		26.8
(-3)
P 		2529
avr 14.3
(-3) 		1714.7
(1.8) 		2151.6
(3) 		24.3
(-3) 		2137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1402
1 2 3 4 5 6 range
A 795.8
(-1)
P 		1329.8
(1.5)
P 		1192.5
(0.9)
P 		1644.7
(3)
P 		1160.9
(0.7)
P 		375
(-3)
P 		1270
B 1524.7
(1.7)
P 		1388
(1)
P 		1091
(-0.6)
P 		1580.2
(2)
P 		1777.4
(3)
P 		636.4
(-3)
P 		1141
C 1684.7
(3)
P 		1275.3
(0.7)
P 		1095.5
(-0.4)
P 		947.8
(-1.2)
P 		1350.3
(1.1)
P 		634.1
(-3)
P 		1051
avr 1335.1
(2.4) 		1331
(2.3) 		1126.3
(0.9) 		1390.9
(2.7) 		1429.5
(3) 		548.5
(-3) 		881
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180376_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5498
Sequence Type Exemplar sequence
Representative Public ID M03A1.3 NCBI
Target Description M03A1.3 /REP_DB=WormBase Gene ID /WP=CE07390 /TR=Q21476 /GB=AAA93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182273(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182273 NCBI Caenorhabditis elegans Dauer or Aging adult Overexpression family member (dao-2) (dao-2) mRNA, complete cds. 11/11 None
SNAP00000019149 ENSEMBL cdna:SNAP chromosome:CEL140:II:4562938:4563437:1 11/11 None
GENEFINDER00000019157 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4562938:4563437:1 11/11 None
M03A1.7.1 ENSEMBL cdna:known chromosome:CEL140:II:4562842:4563533:1 gene:M03A1.7 11/11 None
M03A1.7.2 ENSEMBL cdna:known chromosome:CEL140:II:4562937:4563533:1 gene:M03A1.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4557774-4563436(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dao-2
Ensembl M03A1.7
Entrez Gene 173793 Entrez gene
SwissProt Q86LS4 EMBL-EBI
WormBase CE33427 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO38616 Dauer or aging adult overexpression protein 2 [Caenorhabditis elegans] ref|NP_872073.1| Dauer or Aging adult Overexpression family member (dao-2) [Caenorhabditis elegans] 3.0E-72
blast CAE67858 Hypothetical protein CBG13450 [Caenorhabditis briggsae] 2.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 7.2E-21
Sequence
Target Sequence 
>C. ELEGANS:180376_S_AT
tgtgacttcaatgctctttctcaagctaacattctgaacttcatgtcgacttgcggagag
cgtggaccaactgtcggagaaatgtgggattgtgcttccctccgtcacaaccacgaggac
tgctgcaagaaggccggtgtctctggagaatgcctcaagtactgtaccgctcataaggga
gctccatccaactatttggactatgctttctgtaccgagaacttcaacgagatccgtgat
tgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGACTTCAATGCTCTTTCTCAAG 18 557 2068 Antisense
GACTTCAATGCTCTTTCTCAAGCTA 47 373 2071 Antisense
CAATGCTCTTTCTCAAGCTAACATT 66 183 2076 Antisense
TTCATGTCGACTTGCGGAGAGCGTG 102 679 2107 Antisense
GTCGACTTGCGGAGAGCGTGGACCA 6 475 2112 Antisense
TTGCGGAGAGCGTGGACCAACTGTC 343 693 2118 Antisense
GTACCGCTCATAAGGGAGCTCCATC 401 457 2231 Antisense
AAGGGAGCTCCATCCAACTATTTGG 489 157 2242 Antisense
TGTACCGAGAACTTCAACGAGATCC 342 557 2278 Antisense
ACCGAGAACTTCAACGAGATCCGTG 534 89 2281 Antisense
ACTTCAACGAGATCCGTGATTGCTT 153 103 2288 Antisense
Sequence Source Affymetrix Proprietary Database