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[180375_at]

Description M03A1.3 /REP_DB=WormBase Gene ID /WP=CE07390 /TR=Q21476 /GB=AAA93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 60      4grp_high_8_8 > 54      6grp_highest_nonorm > 06      6grp_total > 61      4grp_total > 49      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15488
1 2 3 4 range
A 374.8
(-3)
P 		10359.5
(1.1)
P 		15024.6
(3)
P 		509
(-2.9)
P 		14650
B 357.4
(-3)
P 		13639
(2.2)
P 		15798
(3)
P 		484.8
(-3)
P 		15441
C 310
(-3)
A 		12933.5
(3)
P 		12105.9
(2.6)
P 		402.9
(-3)
P 		12624
avr 347.4
(-3) 		12310.7
(2.1) 		14309.5
(3) 		465.6
(-2.9) 		13962

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14240
1 2 3 4 5 6 range
A 6598.1
(-0.8)
P 		7254.8
(-0.4)
P 		13312.1
(3)
P 		13208.8
(2.9)
P 		6932.2
(-0.6)
P 		2594.7
(-3)
P 		10717
B 10004.6
(0.8)
P 		7173.9
(-0.7)
P 		11387.7
(1.5)
P 		14302.1
(3)
P 		8481.3
(0)
P 		2641
(-3)
P 		11661
C 8640.5
(-0.4)
P 		7916.6
(-0.8)
P 		8600.2
(-0.5)
P 		16816.9
(3)
P 		8034.7
(-0.7)
P 		2576.8
(-3)
P 		14240
avr 8414.4
(-0.1) 		7448.4
(-0.6) 		11100
(1.2) 		14775.9
(3) 		7816.1
(-0.4) 		2604.2
(-3) 		12172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4151
1 2 3 4 range
A 136.5
(-2.9)
P 		2248.8
(0.4)
P 		3927.7
(3)
P 		74.5
(-3)
P 		3853
B 114
(-3)
P 		3368.5
(2.5)
P 		3660.8
(3)
P 		103.3
(-3)
P 		3558
C 102.6
(-2.9)
A 		3718.5
(2.3)
P 		4219.3
(3)
P 		67.9
(-3)
P 		4151
avr 117.7
(-2.9) 		3111.9
(1.7) 		3935.9
(3) 		81.9
(-3) 		3854

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3156
1 2 3 4 5 6 range
A 1216.3
(-1.7)
P 		2580.2
(1.3)
P 		2899.1
(2)
P 		3350.1
(3)
P 		2091.8
(0.2)
P 		631.7
(-3)
P 		2718
B 3481.1
(2.3)
P 		2387.9
(0)
P 		2765.3
(0.8)
P 		3788.1
(3)
P 		3445.8
(2.3)
P 		984.8
(-3)
P 		2803
C 3202.7
(2.6)
P 		2415.6
(0.7)
P 		2188.9
(0.2)
P 		3381.9
(3)
P 		2599.9
(1.1)
P 		862.6
(-3)
P 		2519
avr 2633.4
(1) 		2461.2
(0.7) 		2617.8
(1) 		3506.7
(3) 		2712.5
(1.2) 		826.4
(-3) 		2680
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180375_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5498
Sequence Type Exemplar sequence
Representative Public ID M03A1.3 NCBI
Target Description M03A1.3 /REP_DB=WormBase Gene ID /WP=CE07390 /TR=Q21476 /GB=AAA93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062404, NM_182273
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182273 NCBI Caenorhabditis elegans Dauer or Aging adult Overexpression family member (dao-2) (dao-2) mRNA, complete cds. A 
M03A1.3 ENSEMBL cdna:known chromosome:CEL140:II:4557776:4562576:1 gene:M03A1.3 None
M03A1.7.1 ENSEMBL cdna:known chromosome:CEL140:II:4562842:4563533:1 gene:M03A1.7 None
M03A1.7.2 ENSEMBL cdna:known chromosome:CEL140:II:4562937:4563533:1 gene:M03A1.7 None
SNAP00000019148 ENSEMBL cdna:SNAP chromosome:CEL140:II:4560394:4562456:1 None
SNAP00000019149 ENSEMBL cdna:SNAP chromosome:CEL140:II:4562938:4563437:1 None
GENEFINDER00000019160 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4560394:4562456:1 None
NM_062404 NCBI Caenorhabditis elegans M03A1.3 (M03A1.3) mRNA, complete cds. None
GENEFINDER00000019157 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4562938:4563437:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4557774-4563436(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Gene Symbol dao-2
Ensembl M03A1.3
M03A1.7
Entrez Gene 173791 Entrez gene
173793 Entrez gene
SwissProt Q21476 EMBL-EBI
Q86LS4 EMBL-EBI
WormBase CE33426 Wormbase
CE33427 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO38615 Hypothetical protein M03A1.3 [Caenorhabditis elegans] ref|NP_494805.2| M03A1.3 [Caenorhabditis elegans] 0.0
blast AAO38615 Hypothetical protein M03A1.3 [Caenorhabditis elegans] ref|NP_494805.2| M03A1.3 [Caenorhabditis elegans] 1.0E-152
blast CAE67867 Hypothetical protein CBG13459 [Caenorhabditis briggsae] 1.0E-143
blast AAO38616 Dauer or aging adult overexpression protein 2 [Caenorhabditis elegans] ref|NP_872073.1| Dauer or Aging adult Overexpression family member (dao-2) [Caenorhabditis elegans] 3.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 7.2E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494805.2 12 136-158,179-201,216-238,251-273,283-305,326-348,439-461,482-504,519-541,554-576,586-608,641-663
M03A1.3 12 136-158,179-201,216-238,251-273,283-305,326-348,439-461,482-504,519-541,554-576,586-608,641-663
SNAP00000019148 6 105-127,148-170,185-207,220-242,252-274,307-329
GENEFINDER00000019160 6 152-174,195-217,232-254,267-289,299-321,354-376
Sequence
Target Sequence 
>C. ELEGANS:180375_AT
tacgtggctgccgccaattatcgaaagacatggtcagagccaacaatggaaattgtgctg
accagtgtgccgaccattttgtgtatcgccacaggattcgtagttttgtatttggaaagc
gagcagagaaatgggtatagggaagttgaaatgatcgaaaaatccgaaaaaaattccaaa
aaaccggagcgtcttcaacttatccaaatctcatggataatgaaccgcttcgcaattctc
ctggctctcccacttcttgctcttgctgctactccaaatgagaagctgaagctatgctgt
gccacgttgaaggatgccgataaggagtgcgtcgatcgattctgtgacttcaatgctctt
tctcaagctaacattctgaacttcatgtcgacttgcggagagcgtggaccaactgtcgga
gaaatgtgggattgtgcttccctccgtcacaaccacgaggactgctgcaagaaggccggt
gtctctggagaatgcctcaagtactgtaccgctcataagggagctccatccaactatttg
gactatgctttctgtaccgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACGTGGCTGCCGCCAATTATCGAA 333 647 1726 Antisense
AAATTGTGCTGACCAGTGTGCCGAC 597 115 1775 Antisense
GTGCCGACCATTTTGTGTATCGCCA 168 479 1792 Antisense
GGAGCGTCTTCAACTTATCCAAATC 512 521 1911 Antisense
ATAATGAACCGCTTCGCAATTCTCC 586 25 1942 Antisense
GCTCTTGCTGCTACTCCAAATGAGA 399 301 1984 Antisense
AGCTGAAGCTATGCTGTGCCACGTT 196 81 2009 Antisense
GTGCGTCGATCGATTCTGTGACTTC 297 479 2052 Antisense
CAAGAAGGCCGGTGTCTCTGGAGAA 575 185 2193 Antisense
GAATGCCTCAAGTACTGTACCGCTC 382 331 2215 Antisense
TTGGACTATGCTTTCTGTACCGAGA 545 707 2263 Antisense
Sequence Source Affymetrix Proprietary Database