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[180371_at]

Description F31E8.1 /REP_DB=WormBase Gene ID /WP=CE01928 /TR=Q19939 /GB=AAA98025.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 18      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 20
(-3)
A 		63.4
(3)
A 		33.2
(-1.2)
A 		60.1
(2.5)
A 		43
B 38.1
(3)
A 		14.2
(-3)
A 		14.5
(-2.9)
A 		23.3
(-0.7)
A 		24
C 4.7
(-3)
A 		17.8
(-2.1)
A 		10.9
(-2.6)
A 		96.3
(3)
A 		92
avr 20.9
(-2.8) 		31.8
(-1.2) 		19.5
(-3) 		59.9
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 45.3
(1.8)
A 		49.1
(2.3)
A 		55.1
(3)
A 		17.3
(-1.6)
A 		5.5
(-3)
A 		7.5
(-2.8)
A 		50
B 21.9
(0.9)
A 		6
(-3)
A 		15.1
(-0.8)
A 		11.9
(-1.6)
A 		30.6
(3)
A 		28.3
(2.4)
A 		25
C 21.2
(-0.7)
A 		42.1
(2.6)
A 		6.1
(-3)
A 		44.9
(3)
A 		6.8
(-2.9)
A 		43.5
(2.8)
A 		39
avr 29.5
(2) 		32.4
(3) 		25.4
(0.7) 		24.7
(0.4) 		14.3
(-3) 		26.4
(1) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 7.3
(-3)
A 		13.8
(3)
A 		8.7
(-1.7)
A 		8.8
(-1.6)
A 		7
B 12.2
(3)
A 		3.5
(-2.9)
A 		3.4
(-3)
A 		5
(-1.9)
A 		9
C 1.5
(-3)
A 		5.1
(-1.5)
A 		3.8
(-2.1)
A 		16.2
(3)
A 		15
avr 7
(-0.8) 		7.5
(-0.2) 		5.3
(-3) 		10
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 8.4
(-0.5)
A 		17.5
(3)
A 		12
(0.9)
A 		4.4
(-2)
A 		1.7
(-3)
A 		1.8
(-3)
A 		16
B 7.6
(0.2)
A 		2
(-3)
A 		3.7
(-2)
A 		3.1
(-2.4)
A 		12.4
(3)
A 		10.5
(1.9)
A 		10
C 7.9
(-0.1)
A 		12.9
(2.2)
A 		1.6
(-3)
A 		9
(0.4)
A 		2.2
(-2.7)
A 		14.6
(3)
A 		13
avr 8
(-0.1) 		10.8
(3) 		5.8
(-2.6) 		5.5
(-2.9) 		5.4
(-3) 		9
(1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180371_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5465
Sequence Type Exemplar sequence
Representative Public ID F31E8.1 NCBI
Target Description F31E8.1 /REP_DB=WormBase Gene ID /WP=CE01928 /TR=Q19939 /GB=AAA98025.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062996(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062996 NCBI Caenorhabditis elegans F31E8.1 (F31E8.1) mRNA, complete cds. 11/11 None
GENEFINDER00000019084 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6828049:6830386:1 11/11 None
F31E8.1 ENSEMBL cdna:known chromosome:CEL140:II:6828049:6830386:1 gene:F31E8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019082 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6828043-6830381(+) 94.13 100.0
Public Domain and Genome References
Gene Title
Ensembl F31E8.1
Entrez Gene 185155 Entrez gene
SwissProt Q19939 EMBL-EBI
WormBase CE01928 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495397 F31E8.1 [Caenorhabditis elegans] gb|AAA98025.1| Hypothetical protein F31E8.1 [Caenorhabditis elegans] 1.0E-120
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495397.1 3 33-55,62-79,165-187
F31E8.1 3 33-55,62-79,165-187
GENEFINDER00000019084 3 33-55,62-79,165-187
Sequence
Target Sequence 
>C. ELEGANS:180371_AT
cttctcgttatgtctctttttcgtgtttttggaggacggtggaccctcgttgtccgccct
cctttacttcttcggtttctttttgcgccacggcgtcacggatggcgagcttctcccacc
cgctccaaatcttgttacattgcggaaagagtctcctcctgccccatctcctcctagccg
tgtcacaccaattagacactctcctctgccagccggcaaactgcgatggggtcctcaagt
accttcacttttcccgcatgtgatcgccacctccggcgacaacgcttatcaccctgatgc
ttcatttgttatcagcactcgtatgtgtttgttctacacgcattctaaccccgacttctt
catctttttcctcttctccatctcctttttcatcttctacgccacttcttctaattcctt
gtcacgcgtgttctgggagtcttcgtcacatctgaaggaatggaaggcagtggaagaaaa
ctatgaaggagtaggaggagaccttctcgagatttacgacttgacaagtagtattggtgg
actagccccggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTCGTTATGTCTCTTTTTCGTG 324 221 150 Antisense
TTTGGAGGACGGTGGACCCTCGTTG 280 663 177 Antisense
CGCCACGGCGTCACGGATGGCGAGC 182 257 235 Antisense
CATTGCGGAAAGAGTCTCCTCCTGC 651 215 287 Antisense
GTCACACCAATTAGACACTCTCCTC 364 465 331 Antisense
ACTGCGATGGGGTCCTCAAGTACCT 495 97 369 Antisense
TTATCACCCTGATGCTTCATTTGTT 239 679 435 Antisense
CTTCATTTGTTATCAGCACTCGTAT 416 221 449 Antisense
GTTTGTTCTACACGCATTCTAACCC 642 449 476 Antisense
GGGAGTCTTCGTCACATCTGAAGGA 425 493 584 Antisense
TAGTATTGGTGGACTAGCCCCGGAT 596 653 678 Antisense
Sequence Source Affymetrix Proprietary Database