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[180353_at]

Description T27E4.1 /REP_DB=WormBase Gene ID /WP=CE14247 /TR=Q23056 /GB=AAB04843.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 82      6grp_total > 82      4grp_total > 12      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 548
1 2 3 4 range
A 186.5
(-1.9)
P 		180.9
(-2)
P 		107.3
(-3)
P 		534.4
(3)
P 		427
B 286.4
(-1.1)
P 		200.1
(-2.2)
P 		132.7
(-3)
P 		610.7
(3)
P 		478
C 285.1
(-1.6)
P 		194.6
(-2.7)
P 		170.6
(-3)
P 		655
(3)
P 		484
avr 252.7
(-1.5) 		191.9
(-2.3) 		136.9
(-3) 		600
(3) 		463

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 236.9
(-2)
P 		296.2
(-0)
P 		385.6
(3)
P 		320.7
(0.8)
P 		357.7
(2.1)
P 		208.9
(-3)
P 		177
B 236.3
(-3)
P 		425.5
(1.2)
P 		294.9
(-1.7)
P 		506.6
(3)
P 		503.4
(2.9)
P 		359.3
(-0.3)
P 		270
C 214.2
(-3)
P 		443.3
(3)
P 		290.3
(-1)
P 		273.9
(-1.4)
P 		369.7
(1.1)
P 		431.4
(2.7)
P 		229
avr 229.1
(-3) 		388.3
(2.3) 		323.6
(0.1) 		367.1
(1.6) 		410.3
(3) 		333.2
(0.4) 		181
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 67.9
(1.8)
P 		39.3
(-1.6)
P 		28
(-3)
P 		78.2
(3)
P 		50
B 91.3
(0.7)
P 		49.4
(-1.9)
P 		30.8
(-3)
P 		130.1
(3)
P 		99
C 94.4
(1.2)
P 		56
(-3)
P 		59.4
(-2.6)
P 		110.4
(3)
P 		54
avr 84.5
(1.1) 		48.2
(-2.2) 		39.4
(-3) 		106.2
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 43.7
(-3)
P 		105.3
(2.8)
P 		84
(0.8)
P 		81.3
(0.5)
P 		107.9
(3)
P 		50.9
(-2.3)
P 		64
B 82.2
(-2.5)
P 		141.6
(0.2)
P 		71.6
(-3)
P 		134.2
(-0.2)
P 		204.5
(3)
P 		134
(-0.2)
P 		133
C 79.4
(-1.4)
P 		135.3
(2.4)
P 		73.9
(-1.7)
P 		55.1
(-3)
P 		119.6
(1.3)
P 		144.4
(3)
P 		89
avr 68.4
(-3) 		127.4
(1.7) 		76.5
(-2.4) 		90.2
(-1.3) 		144
(3) 		109.8
(0.3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180353_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12442
Sequence Type Exemplar sequence
Representative Public ID T27E4.1 NCBI
Target Description T27E4.1 /REP_DB=WormBase Gene ID /WP=CE14247 /TR=Q23056 /GB=AAB04843.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072957(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072957 NCBI Caenorhabditis elegans T27E4.1 (T27E4.1) mRNA, complete cds. 10/11 None
SNAP00000044636 ENSEMBL cdna:SNAP chromosome:CEL140:V:9095284:9096922:1 10/11 None
T27E4.1 ENSEMBL cdna:known chromosome:CEL140:V:9095284:9096932:1 gene:T27E4.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9095284-9096970(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27E4.1
Entrez Gene 188995 Entrez gene
SwissProt Q23056 EMBL-EBI
WormBase CE29357 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04843 Hypothetical protein T27E4.1 [Caenorhabditis elegans] ref|NP_505358.1| T27E4.1 [Caenorhabditis elegans] 1.0E-180
blast CAE72097 Hypothetical protein CBG19188 [Caenorhabditis briggsae] 1.0E-169
blast AAB04843 Hypothetical protein T27E4.1 [Caenorhabditis elegans] ref|NP_505358.1| T27E4.1 [Caenorhabditis elegans] 1.0E-162
blast CAE72097 Hypothetical protein CBG19188 [Caenorhabditis briggsae] 1.0E-154
Sequence
Target Sequence 
>C. ELEGANS:180353_AT
actcttcttcgtattcaaatctgtgatatcgacgagtttgatcagaatgtgattgtggca
gaaatggattactggataaacacacatatcatatcacaattcacacaatttgaattgcca
ttccctccatttacaccggatattggagaaattgagttacacatgacatatctaccaact
tcacaacgacttatcttgcattattcaactgtgacaaacttgaaattggacgaaagttgc
agtgttttaacaatagtttttgggaggcgctttttttttaatttttcaaacgtttctcat
aaatatttttcagcaatattcattcgtggcgtcatacggcgattggaaaatgcttgtaca
gatgacggaatgcatttcactcacaagttgatttttgatttagatcgaaaatatgttccc
aaagctgaagtaattattcatgtgctgcaagttacatctggtggtcgggcaggtgtcact
ttcaatgcttcactcgggtcaatgccagcaaatgctcaaatctttgaggacgacaatttc
acgtgttcatcggttaactccaagtgctggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCTTCTTCGTATTCAAATCTGTG 219 101 382 Antisense
CAATTTGAATTGCCATTCCCTCCAT 580 179 487 Antisense
ATTCCCTCCATTTACACCGGATATT 261 9 501 Antisense
ATATCTACCAACTTCACAACGACTT 683 19 549 Antisense
CATTCGTGGCGTCATACGGCGATTG 203 217 702 Antisense
GACGGAATGCATTTCACTCACAAGT 299 377 745 Antisense
GCAAGTTACATCTGGTGGTCGGGCA 525 319 828 Antisense
GGGCAGGTGTCACTTTCAATGCTTC 375 493 848 Antisense
CAATGCTTCACTCGGGTCAATGCCA 553 181 864 Antisense
GGACGACAATTTCACGTGTTCATCG 700 523 909 Antisense
TCATCGGTTAACTCCAAGTGCTGGT 404 619 928 Antisense
Sequence Source Affymetrix Proprietary Database