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[180342_at]

Description F35E12.6 /REP_DB=WormBase Gene ID /WP=CE09921 /TR=O02358 /GB=CAB04273.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 23      6grp_total > 31      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3550
1 2 3 4 range
A 3416.6
(3)
P 		2534.6
(1.4)
P 		3353.6
(2.9)
P 		78.8
(-3)
P 		3338
B 2617.5
(2.7)
P 		2765.7
(3)
P 		2715.9
(2.9)
P 		99.9
(-3)
P 		2666
C 3369.9
(2.6)
P 		2641.6
(1.4)
P 		3617.6
(3)
P 		67.8
(-3)
P 		3550
avr 3134.7
(2.8) 		2647.3
(1.9) 		3229
(3) 		82.2
(-3) 		3147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2528
1 2 3 4 5 6 range
A 1835
(0.3)
P 		2667.5
(2.8)
P 		2717.9
(3)
P 		2311.4
(1.8)
P 		1579.3
(-0.4)
P 		735.7
(-3)
P 		1982
B 2627.2
(1.4)
P 		2663.8
(1.5)
P 		3263.3
(3)
P 		2644.2
(1.5)
P 		1654.9
(-0.9)
P 		804.5
(-3)
P 		2459
C 2479
(1.4)
P 		2570.5
(1.6)
P 		3076.9
(3)
P 		2783.6
(2.2)
P 		1555
(-1.1)
P 		865.5
(-3)
P 		2211
avr 2313.7
(1.1) 		2633.9
(2) 		3019.4
(3) 		2579.7
(1.8) 		1596.4
(-0.9) 		801.9
(-3) 		2218
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1249
1 2 3 4 range
A 1244
(3)
P 		550.2
(-0.4)
P 		876.7
(1.2)
P 		11.5
(-3)
P 		1233
B 834.7
(3)
P 		683.1
(1.9)
P 		629.3
(1.5)
P 		21.3
(-3)
P 		813
C 1115.5
(2.3)
P 		759.5
(0.6)
P 		1260.8
(3)
P 		11.4
(-3)
P 		1249
avr 1064.7
(3) 		664.3
(0.7) 		922.3
(2.2) 		14.7
(-3) 		1050

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 770
1 2 3 4 5 6 range
A 338.3
(-1.8)
P 		948.7
(3)
P 		591.9
(0.2)
P 		586.2
(0.2)
P 		476.5
(-0.7)
P 		179.1
(-3)
P 		770
B 914.2
(3)
P 		886.7
(2.7)
P 		792.4
(1.8)
P 		700.3
(0.9)
P 		672.4
(0.6)
P 		300
(-3)
P 		614
C 918.8
(3)
P 		784.3
(1.7)
P 		783.1
(1.7)
P 		559.8
(-0.4)
P 		503.2
(-1)
P 		289.7
(-3)
P 		629
avr 723.8
(1.5) 		873.2
(3) 		722.5
(1.5) 		615.4
(0.5) 		550.7
(-0.1) 		256.3
(-3) 		617
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180342_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13062
Sequence Type Exemplar sequence
Representative Public ID F35E12.6 NCBI
Target Description F35E12.6 /REP_DB=WormBase Gene ID /WP=CE09921 /TR=O02358 /GB=CAB04273.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074000(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074000 NCBI Caenorhabditis elegans F35E12.6 (F35E12.6) mRNA, complete cds. 11/11 None
SNAP00000032232 ENSEMBL cdna:SNAP chromosome:CEL140:V:13736974:13738741:-1 10/11 None
GENEFINDER00000032243 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13729622:13738741:-1 10/11 None
F35E12.6 ENSEMBL cdna:known chromosome:CEL140:V:13736708:13738741:-1 gene:F35E12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13736681-13738741(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E12.6
Entrez Gene 179866 Entrez gene
SwissProt O02358 EMBL-EBI
WormBase CE35523 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04273 Hypothetical protein F35E12.6 [Caenorhabditis elegans] ref|NP_506401.2| F35E12.6 [Caenorhabditis elegans] 0.0
blast CAB04275 Hypothetical protein F35E12.7a [Caenorhabditis elegans] ref|NP_001023877.1| F35E12.7a [Caenorhabditis elegans] 0.0
blast CAI91171 Hypothetical protein F35E12.7c [Caenorhabditis elegans] ref|NP_001023879.1| F35E12.7c [Caenorhabditis elegans] 0.0
blast CAI91172 Hypothetical protein F35E12.7d [Caenorhabditis elegans] ref|NP_001023880.1| F35E12.7d [Caenorhabditis elegans] 0.0
blast CAB04275 Hypothetical protein F35E12.7a [Caenorhabditis elegans] ref|NP_001023877.1| F35E12.7a [Caenorhabditis elegans] 1.0E-124
blast CAI91170 Hypothetical protein F35E12.7b [Caenorhabditis elegans] ref|NP_001023878.1| F35E12.7b [Caenorhabditis elegans] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 3.6E-26
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 6.9E-11
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 2.6E-26
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.7E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032243 1 1407-1429
Sequence
Target Sequence 
>C. ELEGANS:180342_AT
gaagattcactgagctttaccatgcaccacaatatgcaatttactccacttctaatactc
tcacattcttgaacttgtaccgtactccatctaattcaatgttacttgcaaacgacgcat
cagtcatcgaaaactatgaagtttacaacattatggttctggactctgtcaacccgtatt
ctggatacatgtccaactactatgatgatgctgatgcatactttactgtcatttgtgatg
gttgctccagtattaacatcaaaactctgattttcgattcaaagtataagaatggcgccg
gatatctcgaggtcgctgaaatgtccccaactcaaaaacttccagcaaagctcaactacc
cagcatccacaagcccaagcaaccgtctcccacaattgatttcttcacaaatggcaactt
tccacactttcaatgcacgtgtcacgtatggcattcaagttcggttcggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGATTCACTGAGCTTTACCATGC 685 337 596 Antisense
CAATATGCAATTTACTCCACTTCTA 354 181 625 Antisense
CACTTCTAATACTCTCACATTCTTG 683 195 642 Antisense
GTACCGTACTCCATCTAATTCAATG 173 459 672 Antisense
ACTCTGTCAACCCGTATTCTGGATA 677 99 758 Antisense
GATGCTGATGCATACTTTACTGTCA 693 413 802 Antisense
TAAGAATGGCGCCGGATATCTCGAG 12 639 882 Antisense
TCTCGAGGTCGCTGAAATGTCCCCA 628 609 900 Antisense
TCCAGCAAAGCTCAACTACCCAGCA 274 587 936 Antisense
GTCTCCCACAATTGATTTCTTCACA 388 471 980 Antisense
ATGGCATTCAAGTTCGGTTCGGAGT 339 53 1043 Antisense
Sequence Source Affymetrix Proprietary Database